Development of keratin–chitosan–gelatin composite scaffold for soft tissue engineering

Kakkar, Prachi; Verma, Sudhanshu; Manjubala, I.; Madhan, Balaraman

doi:10.1016/j.msec.2014.09.021

Cited by 107 publications

(55 citation statements)

References 28 publications

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“…Scaffold porosity was determined via an ethanol infiltration assay . A 4‐mm‐diameter biopsy punch was removed from dry scaffolds ( n = 6) and trimmed to obtain a cylindrical sample.…”

Section: Methodsmentioning

confidence: 99%

Biomechanical characterization of a novel collagen‐hyaluronan infused 3D‐printed polymeric device for partial meniscus replacement

et al. 2019

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The menisci transmit load by increasing the contact area and decreasing peak contact stresses on the articular surfaces. Meniscal lesions are among the most common orthopedic injuries, and resulting meniscectomies are associated with adverse polycaprolactone contact mechanics changes and, ultimately, an increased likelihood of osteoarthritis. Meniscus scaffolds were fabricated by 3D‐printing a network of circumferential and radial filaments of resorbable polymer (poly(desaminotyrosyl‐tyrosine dodecyl ester dodecanoate)) and infused with collagen‐hyaluronan. The scaffold demonstrated an instantaneous compressive modulus (1.66 ± 0.44 MPa) comparable to native meniscus (1.52 ± 0.59 MPa). The scaffold aggregate modulus (1.33 ± 0.51 MPa) was within 2% of the native value (1.31 ± 0.36 MPa). In tension, the scaffold displayed a comparable stiffness to native tissue (127.6–97.1 N/mm) and an ultimate load of 33% of the native value. Suture pull‐out load of scaffolds (83.1 ± 10.0 N) was within 10% of native values (91.5 ± 15.4 N). Contact stress analysis demonstrated the scaffold reduced peak contact stress by 60–67% and increased contact area by 38%, relative to partial meniscectomy. This is the first meniscal scaffold to match both the axial compressive properties and the circumferential tensile stiffness of the native meniscus. The improvement of joint contact mechanics, relative to partial meniscectomy alone, motivates further investigation using a large animal model. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2457–2465, 2019.

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“…Scaffold porosity was determined via an ethanol infiltration assay . A 4‐mm‐diameter biopsy punch was removed from dry scaffolds ( n = 6) and trimmed to obtain a cylindrical sample.…”

Section: Methodsmentioning

confidence: 99%

Biomechanical characterization of a novel collagen‐hyaluronan infused 3D‐printed polymeric device for partial meniscus replacement

et al. 2019

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“…Chitosan is a product of deacetylation of chitin, which exist in crab and shrimp shells, and cuticles of insects. Studies have confirmed its biocompatibility, wound healing capability and antibacterial activities . The incorporation of chitosan gives the keratin based composites multiple functions, especially the antibacterial property, and extends its application to more disciplines.…”

Section: Introductionmentioning

confidence: 90%

“…Tran and Mututuvari developed a composite material using cellulose, chitosan and keratin, and investigated its application in drug release . Similarly, gelatin/chitosan/keratin, starch/chitosan/keratin and PLA/chitosan/keratin composites were also prepared respectively, and applied in the biomedical field . Chitosan is a product of deacetylation of chitin, which exist in crab and shrimp shells, and cuticles of insects.…”

Section: Introductionmentioning

confidence: 99%

Tunable wettability and tensile strength of chitosan membranes using keratin microparticles as reinforcement

Chen

Qiao

et al. 2016

J of Applied Polymer Sci

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Keratin particles with microscale are prepared by ball mill and its influences on the chitosan membrane is evaluated. Composite membranes with various content of keratin are fabricated, and their physical and chemical properties such as morphology, wettability, crystallization, thermal stability, tensile strength, and break elongation are investigated. Optical microscope and situ topographic scan mode of nano‐test system are used to examine the dispersion and aggregation of keratin on the surface of chitosan membrane. The result of contact angle (CA) and mechanical testing show that the incorporation of keratin particles decrease the CA from 98.1° to 58.2°. Tensile strength and break elongation of the composite membrane reaches a maximum of 65 ± 8 MPa and 15% when the keratin content is 6%, an increase of 80% and 88% compared with the pristine chitosan membrane. Both the increase in tensile strength and break elongation is result of the incorporation of keratin particles known for their excellent compatibility between keratin and chitosan matrix. These kind of composite material combines the antibacterial properties of chitosan with cell culture preference of keratin which may have potential biomedical application. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 44667.

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“…Generally, chitosan is described in detail by the average molecular weight (Mw) and the degree of deacetylation. It is unstable in the low pH value environment, therefore, oppositely charged polysaccharides such as alginate, gellan gum, κ‐carrageen, and pectin were used to form polyelectrolyte complexes (PECs) for overcoming the instability …”

Section: Introductionmentioning

confidence: 99%

Surface modification by deposition of IPA plasma and gellan gum/chitosan hybrid hydrogel onto thermoplastic polyurethane for controlled release of N‐acetylcysteine

Pan

Liu

Ting

et al. 2019

J Chinese Chemical Soc

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A thin film system composed of gellan gum and chitosan was fabricated through a combination of polyelectrolyte blend and hybrid hydrogel gelation for controlled release of drug. In this study, precursor isopropyl alcohol (IPA) was used to plasma deposit on the surface of thermoplastic polyurethane (TPU) to form a hydrophilic film. The features of the thin film were evaluated using water contact angle (WCA) measurement, scanning electron microscopy (SEM), Fourier transform infra‐red (FTIR), UV/Vis spectroscopy, and studies of controlled release of drugs. The hybrid hydrogel, pH‐sensitive, was tested at pH values of 1.2 and 7.4 of buffer solution and at a temperature of 37°C to observe its swelling ratio and drug delivery properties with N‐acetylcysteine as a drug material for controlled release. Furthermore, at pH 7.4, the hybrid hydrogel has an outstanding release ratio of up to about 90% absorption amounts of N‐acetylcysteine after 8 hr. The mechanism of drug release from thin film devices (n = 0.684) is anomalous (non‐Fickian) transport, the value of n lies between 0.43 and 0.85.

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Development of keratin–chitosan–gelatin composite scaffold for soft tissue engineering

Cited by 107 publications

References 28 publications

Biomechanical characterization of a novel collagen‐hyaluronan infused 3D‐printed polymeric device for partial meniscus replacement

Biomechanical characterization of a novel collagen‐hyaluronan infused 3D‐printed polymeric device for partial meniscus replacement

Tunable wettability and tensile strength of chitosan membranes using keratin microparticles as reinforcement

Surface modification by deposition of IPA plasma and gellan gum/chitosan hybrid hydrogel onto thermoplastic polyurethane for controlled release of N‐acetylcysteine

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