Development of isoproterenol-indueed cardiac hypertrophy

Taylor, Paul B.; Tang, Qian

doi:10.1139/y84-061

Cited by 55 publications

(48 citation statements)

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“…One of the best documented of these is the increase in the RNA content per cell in overload-induced cardiac hypertrophy. Cardiac overload induced by either aortic [8,44] or pulmonary [34] constriction, or by isoproterenol injection [45] results in an early hypertrophic response within 1 4 days that is associated with a 30-50% increase in cardiac total RNA content, but no significant change in cardiac DNA content. Overload of stretched skeletal muscle in rabbits induced by electrical stimulation results in a rapid hypertrophic response that is associated with a 250% increase in RNA content [15].…”

Section: Discussionmentioning

confidence: 99%

Absolute concentrations of mRNA for type I and type VI collagen in the canine meniscus in normal and ACL‐deficient knee joints obtained by RNase protection assay

Wildey

Billetz

Matyas

et al. 2001

Journal Orthopaedic Research

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Relatively little is known about the cellular and molecular responses of the knee joint meniscus to joint injury, despite the functional importance of the tissue. We investigated how meniscus cells respond to joint injury in the early stages of post-traumatic osteoarthritis by characterizing the changes in matrix gene expression in menisci at 3 and 12 weeks post-surgery in dogs in which the anterior cruciate ligament (ACL) in one joint was transected and the other unoperated joint served as a control. Changes in the total RNA and DNA concentrations of the menisci were determined. Absolute concentrations of the mRNA of the COLlAl gene of type 1 collagen, the major fibrillar collagen of the meniscus, and the COL6A3 gene of type V1 collagen, a major repair molecule, were determined by quantitative ribonuclease (RNasef protection assay. The concentration of total RNA in medial and lateral menisci increased from 40 to 60 pg RNAlg wet wt in unoperated, control joints to 200-350 pg R N N g wet wt in ACL-deficient joints. No significant changes were detected in the concentration of DNA (900-1200 pg DNA/g wet wt). Low concentrations of COLlAl (2-3 pmol mRNNg DNA) and COL6A3 (0.3-0.6 pmol mRNA/g DNA) mRNA transcripts were measured in normal menisci. ACLdeficiency induced a 20-38 fold increase in COLlAl and COL6A3 mRNA concentration at 3 weeks, and an 11-19 fold increase at 12 weeks post-surgery. In general, the increase in COLlAl and COL6A3 mRNA concentrations was greater in medial menisci than in lateral menisci. These results demonstrate that the menisci initiate a vigorous biosynthetic response to transection of the ACL.

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Section: Discussionmentioning

confidence: 99%

Absolute concentrations of mRNA for type I and type VI collagen in the canine meniscus in normal and ACL‐deficient knee joints obtained by RNase protection assay

Wildey

Billetz

Matyas

et al. 2001

Journal Orthopaedic Research

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“…For example, hyperactivity of the sympathetic nervous system induced by sinoaortic denervation (6,7) even in the acute phase is capable of eliciting LVH in male rats (8). Furthermore, chronic administration of the sympathomimetic drug isoproterenol produces cardiac hypertrophy in rats (9)(10)(11)(12). However, little is known about the influence of renal sympathetic nerve activity on LVH.…”

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confidence: 99%

Diverse effects of renal denervation on ventricular hypertrophy and blood pressure in DOCA-salt hypertensive rats

Cabral

Silva

Gardioli

et al. 1998

Braz J Med Biol Res

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Cardiac hypertrophy that accompanies hypertension seems to be a phenomenon of multifactorial origin whose development does not seem to depend on an increased pressure load alone, but also on local growth factors and cardioadrenergic activity. The aim of the present study was to determine if sympathetic renal denervation and its effects on arterial pressure level can prevent cardiac hypertrophy and if it can also delay the onset and attenuate the severity of deoxycorticosterone acetate (DOCA)-salt hypertension. DOCA-salt treatment was initiated in rats seven days after uninephrectomy and contralateral renal denervation or sham renal denervation. DOCA (15 mg/kg, sc) or vehicle (soybean oil, 0.25 ml per animal) was administered twice a week for two weeks. Rats treated with DOCA or vehicle (control) were provided drinking water containing 1% NaCl and 0.03% KCl. At the end of the treatment period, mean arterial pressure (MAP) and heart rate measurements were made in conscious animals. Under ether anesthesia, the heart was removed and the right and left ventricles (including the septum) were separated and weighed. DOCA-salt treatment produced a significant increase in left ventricular weight/body weight (LVW/BW) ratio (2.44 ± 0.09 mg/g) and right ventricular weight/body weight (RVW/BW) ratio (0.53 ± 0.01 mg/g) compared to control (1.92 ± 0.04 and 0.48 ± 0.01 mg/g, respectively) rats. MAP was significantly higher (39%) in DOCA-salt rats. Renal denervation prevented (P>0.05) the development of hypertension in DOCA-salt rats but did not prevent the increase in LVW/BW (2.27 ± 0.03 mg/g) and RVW/BW (0.52 ± 0.01 mg/g). We have shown that the increase in arterial pressure level is not responsible for cardiac hypertrophy, which may be more related to other events associated with DOCA-salt hypertension, such as an increase in cardiac sympathetic activity. Correspondence

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“…In this context, Taylor and Tang (1984) reported that maximum HW can be reached on the eighth day of subcutaneous ISO administration. The observed increase of HW in ISOinjected rats might be attributed to the increase in water content, edema of the interstitium, and inflammatory cellular infiltration due to extensive necrosis of cardiac muscle (Patel et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Down‐regulation of CD68 after simvastatin treatment of isoproterenol‐induced myocardial infarction in rats

Atteya

2016

Int. j. adv. appl. sci.

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Isoproterenol (ISO) induces myocardial injuries in the form of ischemia and infarction (MI). Simvastatin (SIM) is a lipid-soluble inhibitor of hydroxy-3-methylglutaryl coenzyme A reductase with multiple reported therapeutic benefits. The present study was designed to evaluate the effect of pretreatment with SIM on ISO-induced cardiac infarction in rats. Forty-eight rats were divided into four groups. Group I (control) received normal saline. Group II (SIM) received SIM (10 mg/kg body weight, orally by gavage) for 30 days. Group III (ISO) received ISO (5 mg/kg) intraperitoneally for 7 days to induce cardiac injury. Group IV (ISO/SIM); received SIM (10 mg/kg body weight, orally by gavage) for 30 days and in the last 7 days they received ISO (5 mg/kg) intraperitoneally. Serological analysis for detection of cardiac injury markers (troponin-T and creatine phosphokinase-MB "CPK-MB") and inflammatory markers (IL-6 and TNF-α) was done. Cardiac tissues were processed for histological examination (H&E and Masson's trichrome) and for the immunohistological quantitative analysis of CD68. Administration of ISO induced an increase in heart weight to body weight (HW/BW) ratio and elevation of systolic and diastolic blood pressure. Serological analysis revealed an increase of interleukin-6, troponin-T, (CPK-MB), and tumor necrosis factor-α (TNF-α). Histopathological examination of heart tissue revealed thickening of the left ventricle and inter-ventricular septum, large focal areas of sub-endocardial degeneration, mononuclear cellular infiltrations, and massive interstitial fibrosis. In addition, ISO-treated rats exhibited significant up-regulation of CD68. Pre-treatment with SIM significantly attenuated ISO-induced cardiac hypertrophy and necrosis, alleviated the elevated biochemical parameters and CD68 expression, and improved the heart histopathological changes. This study provides evidence that SIM minimizes the ischemic effect of ISO on the heart of rats through inhibition of inflammatory cellular infiltration, especially macrophages, as confirmed by down-regulation of CD68.

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Development of isoproterenol-indueed cardiac hypertrophy

Cited by 55 publications

References 0 publications

Absolute concentrations of mRNA for type I and type VI collagen in the canine meniscus in normal and ACL‐deficient knee joints obtained by RNase protection assay

Absolute concentrations of mRNA for type I and type VI collagen in the canine meniscus in normal and ACL‐deficient knee joints obtained by RNase protection assay

Diverse effects of renal denervation on ventricular hypertrophy and blood pressure in DOCA-salt hypertensive rats

Down‐regulation of CD68 after simvastatin treatment of isoproterenol‐induced myocardial infarction in rats

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