Development of insect-resistant transgenic cauliflower plants expressing the trypsin inhibitor gene isolated from local sweet potato

Ding, Lixia; Hu, Chao; Yeh, Kai‐Wun; Wang, P.-J.

doi:10.1007/s002990050497

Cited by 62 publications

(27 citation statements)

References 17 publications

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“…The expression of trypsin inhibitor can provide an efficient method for crop protection. The trypsin inhibitor proteins produced in the transgenic cauliflower plants were functionally active in planta resistance to Pieris conidia and Plutella xylostella (Ding et al, 1998). The barley trypsin inhibitor expressed in transgenic rice seeds provided significant protection against the coleopteran rice weevil Sitophilus oryzae, one of the most important insect storage pests of rice (Julio et al, 2003).…”

Section: Discussionmentioning

confidence: 96%

Differential Proteomic Analysis of the Resistant Soybean Infected by Soybean Cyst Nematode, Heterodera glycines Race 3

Liu¹,

Chen²,

Duan³

2011

JAS

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Plant parasitic nematode, Heterodera glycines (Soybean cyst nematode, SCN) is the major pathogen of Glycine max (soybean). This pathogen is widely distributed, seriously disserving, and has a wide approach for spreading. Huipizhi Heidou (ZDD2315), a germplasm resource of soybean originated in China, has an excellent resistance to SCN. In this paper, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were employed to separate the differentially expressed proteins from soybean induced by SCN. The F 4 separated populations from the cross between the resistant Huipizhi Heidou and the susceptible Liaodou 15 were used as test materials by bulked segregant analysis. The 2-DE gels analysis revealed 367 protein spots from the resistant samples and 372 protein spots from the sensitive samples. Among those protein spots, 23 protein spots from the resistant samples and 4 protein spots from the sensitive samples were differentially expressed, and then were selected for peptide mass fingerprinting (PMF) and sequencing assay by MS. Of the 27 differentially expressed proteins, 16 protein spots were identified by MALDI-TOF-MS and 11 were not identified due to low scores. Further analysis showed that the majority of these 16 proteins were involved in defense, energy and metabolism, suggesting that they might be related to the soybean resistance to SCN.

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Section: Discussionmentioning

confidence: 96%

Differential Proteomic Analysis of the Resistant Soybean Infected by Soybean Cyst Nematode, Heterodera glycines Race 3

Liu¹,

Chen²,

Duan³

2011

JAS

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“…De Block et al (1989) used hypocotyl explants to infect with a disarmed Agrobacterium, but their procedure could not be reproduced in other laboratories (Metz et al 1995;Bhalla & Smith 1998). The marker genes and useful trait genes were successfully transferred into cauliflowers by Eimert & Siegemund (1992), Christey et al (1997), Bhalla & Smith (1998), Ding et al (1998), Puddephat et al (2001 and Chakrabarty et al (2002). However, the lower transformation efficiency was a major obstacle in Agrobacterium-mediated transformation of cauliflower where transformation efficiencies were only 2.13% (Passelegue & Kerlan 1996) and 2.2-9.5% (Puddephat et al 2001;Bhalla & Singh 2008).…”

Section: Introductionmentioning

confidence: 93%

A highly efficient in vitro plant regeneration andAgrobacterium-mediated transformation ofBrassica oleraceavar.botrytis

Yao

Zhao

et al. 2010

New Zealand Journal of Crop and Horticultural Science

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A highly efficient in vitro plant regeneration and Agrobacterium-mediated transformation of Brassica oleracea var. botrytis A reliable and efficient regeneration protocol from the cotyledons and hypocotyls is successfully established using three cauliflower genotypes. Thidiazuron (TDZ) is found to be an effective inducer of shoot formation. Both the hypocotyl and cotyledon segments are highly responsive (100%) to shoot formation and produce a maximum of 15.8 shoots per hypocotyl explant on MS salts with B 5 vitamins medium containing TDZ at 0.2 mg L (1 and a-naphthaleneacetic acid at 0.01 mg L (1 . Using this efficient regeneration protocol, the reporter b-glucuronidase (GUS) under the constitutive cauliflower mosaic virus (CaMV) 35S promoter is introduced into cauliflower cells of hypocotyls and cotyledons with Agrobacterium tumefaciens strain EHA105. By optimizing the parameters on the procedure of Agrobacterium-mediated transformation, a high transformation efficiency is demonstrated. The transformation efficiencies are 21.6% and 18.3% for cotyledons and hypocotyls, respectively. Integration of the transgenes in the plant genome is demonstrated by PCR and Southern hybridization analysis. Expression of the transgenes is confirmed by RT-PCR analysis and GUS staining. Compared with previous works, the present study provides higher regeneration and transformation efficiencies and establishes a highly efficient transformation system in cauliflower.

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“…Importantly, sporamin can be induced through wounding and other stress-related chemicals (Ohto et al 1992;Wang et al 2002). There are several successful cases of transformation by Agrobacterium tumefaciens to enhance the insect resistance in transgenic crops (Cai et al 2003;Ding et al 1998;Yeh et al 1997b). However, the level of sporamin expression driven by CaMV35S promoter gradually declines with further propagation.…”

Section: Introductionmentioning

confidence: 97%

Transgenic Brassica napus L. lines carrying a two gene construct demonstrate enhanced resistance against Plutella xylostella and Sclerotinia sclerotiorum

Liu

Guo

Naeem

et al. 2010

Plant Cell Tiss Organ Cult

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Plant diseases and insect pests are serious threat to the growth and yield of oilseed rape. In this study, a binary vector carrying sporamin and chitinase PjChi-1 genes in tandem was introduced into Brassica napus cv. ZS 758 via Agrobacterium tumefaciens for dual resistance against disease and insect attack. Thirty-two regenerated plantlets exhibiting hygromycin resistance were selected following Agrobacterium-mediated transformation of 600 leaf petiole explants. Of these, 27 transformants were confirmed to carry the two transgenes as detected by polymerase chain reaction (PCR) with 4.5% transformation efficiency. Eight plantlets were randomly selected for further confirmation by Southern and northern blot hybridization analyses. Four plants carried single copy of the transgenes, while the remaining four plants carried either two or three copies of the transgenes. Moreover, expression of the sporamin transgene was detected by northern blot hybridization in transgenic lines, but not in wild-type plants. These eight T 0 plants were grown in vitro, and inoculated with the Lepidoptera larvae of Plutella xylostella and with spores of the fungal pathogen of Sclerotinia sclerotiorum. Transgenic plants exhibited high levels of resistance to P. xylostella and S. sclerotiorum when compared to untransformed wild-type plants. Genetic analysis of T 1 progeny confirmed Mendelian segregation of the introduced genes. Therefore, these transgenic lines demonstrate a promising potential for variety development of oilseed rape lines with enhanced resistance against both P. xylostella and S. sclerotiorum.

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Development of insect-resistant transgenic cauliflower plants expressing the trypsin inhibitor gene isolated from local sweet potato

Cited by 62 publications

References 17 publications

Differential Proteomic Analysis of the Resistant Soybean Infected by Soybean Cyst Nematode, Heterodera glycines Race 3

Differential Proteomic Analysis of the Resistant Soybean Infected by Soybean Cyst Nematode, Heterodera glycines Race 3

A highly efficient in vitro plant regeneration andAgrobacterium-mediated transformation ofBrassica oleraceavar.botrytis

Transgenic Brassica napus L. lines carrying a two gene construct demonstrate enhanced resistance against Plutella xylostella and Sclerotinia sclerotiorum

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