Differential Proteomic Analysis of the Resistant Soybean Infected by Soybean Cyst Nematode, Heterodera glycines Race 3

Abstract

Plant parasitic nematode, Heterodera glycines (Soybean cyst nematode, SCN) is the major pathogen of Glycine max (soybean). This pathogen is widely distributed, seriously disserving, and has a wide approach for spreading. Huipizhi Heidou (ZDD2315), a germplasm resource of soybean originated in China, has an excellent resistance to SCN. In this paper, two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were employed to separate the differentially expressed proteins from soybean induced by SCN. The F 4 separated populations from the cross between the resistant Huipizhi Heidou and the susceptible Liaodou 15 were used as test materials by bulked segregant analysis. The 2-DE gels analysis revealed 367 protein spots from the resistant samples and 372 protein spots from the sensitive samples. Among those protein spots, 23 protein spots from the resistant samples and 4 protein spots from the sensitive samples were differentially expressed, and then were selected for peptide mass fingerprinting (PMF) and sequencing assay by MS. Of the 27 differentially expressed proteins, 16 protein spots were identified by MALDI-TOF-MS and 11 were not identified due to low scores. Further analysis showed that the majority of these 16 proteins were involved in defense, energy and metabolism, suggesting that they might be related to the soybean resistance to SCN.

Integrated Omic Approaches Reveal Molecular Mechanisms of Tolerance during Soybean and Meloidogyne incognita Interactions

“…This trend has also been reported for other transcriptomes of soybean genotypes infected with another type of nematode, the soybean cyst nematode ( Heterodera glycines ) [ 80 , 81 , 82 ]. At the protein level, however, the number of DEPs seems to be similar to other soybean studies [ 83 , 84 , 85 ].…”

Diseases of Edible Oilseed Crops

“…Molecular mapping of QTL for resistance to SCN and MAS for breeding for SCN resistance have proven useful in order to assist in the development of SCN-resistant soybean cultivars at many major soybean breeding research institutes in the world (Arelli et al 2010, Delheimer et al 2010, Carter et al 2011, Ferreira et al 2011, Kim et al 2011, Liu et al 2011a,b, Mazarei et al 2011, Vuong et al 2011, Wu and Duan 2011, Arriagada et al 2012, Yuan et al 2012.…”

Proteomic dissection of plant responses to various pathogens

“…revealed 367 and 372 responsive proteins in resistant and susceptible genotypes of soybean plants, respectively, using 2DE analysis. The successfully identified proteins were mostly involved in plant defense, energy, and metabolic processes . Masertia et al.…”