Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect Fusarium verticillioides in Poultry Feed Samples

Omori, Aline Myuki; Ono, Elisabete Yurie Sataque; Hirozawa, Melissa Tiemi; Suguiura, Igor Massahiro de Souza; Hirooka, Elisa Yoko; Fungaro, Maria Helena Pelegrinelli; Ono, Mário Augusto

doi:10.3390/toxins11010048

Cited by 8 publications

(2 citation statements)

References 41 publications

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“…Quantitative PCR (qPCR), which reveals the amount of DNA of one or a group of closely related microorganisms is quite popular in plant pathological research [15,16,17]. Another indirect method for assessing the toxicity of grain is the immunochemical discovery of antigens (EIA) that are produced by fungi during their vital activity [18,19,20,21].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia

Gagkaeva

Gavrilova

Орина

et al. 2019

Toxins

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Wheat grains collected in three regions of Russia—Volga, Ural, and West Siberia—were analyzed for triangulation of methods in analysis of toxigenic Fusarium species. The presence of fungi and quantitative content of their biomass were detected by using various analytical methods, including a mycological and immunochemical methods, and quantitative PCR. Additionally, an enzyme-linked immunosorbent assay and high-performance liquid chromatography with tandem mass spectrometry were applied for determination of mycotoxins. Regional differences were found regarding the contamination of wheat grain by Fusarium fungi and their toxins. The most important observation was the detection of F. graminearum in the Ural and West Siberian regions, where this pathogen had not been found previously. A maximum damaged grains by F. graminearum and F. sporotrichioides was found in the grain samples from West Siberia. The DNA of F. graminearum was detected in 19.2% and DNA of F. sporotrichioides was found in 84.1% of the analyzed grain samples. The amount of Fusarium antigens in the grain samples from the West Siberian region was 7–8 times higher than in the grain samples from the other two regions. Significant contamination of the grain with deoxynivalenol and T-2/HT-2 toxins (maximum contents were 2239 ppb and 199 ppb, respectively) was detected in the West Siberian region.

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Section: Introductionmentioning

confidence: 99%

Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia

Gagkaeva

Gavrilova

Орина

et al. 2019

Toxins

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“…Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) is an ideal immunoassay method for large-scale screening to detect mycotoxin contaminants [11][12][13]. However, the matrix effect that occurs with complicated samples has always been a challenge, in which ic-ELISA results in false-positive or negative results [14].…”

Section: Introductionmentioning

confidence: 99%

Development of a Reliable ic-ELISA with a Robust Antimatrix Interference Capability Based on QuEChERS Technology for the Rapid Detection of Zearalenone in Edible and Medical Coix Seeds and Subsequent Risk Assessments

Guan

Huang

et al. 2022

Foods

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Indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) is an ideal immunoassay method for large-scale screenings to detect mycotoxin contaminants. However, the matrix effect of complicated samples has always been challenging when performing immunoassays, as it leads to false-positive or negative results. In this study, convenient QuEChERS technology combined with optimizing the dilution solvent was ingeniously used to eliminate interference from the sample matrix to greatly improve the detection accuracy, and reliable ic-ELISAs for the two official tolerance levels of 60 and 500 μg/kg were developed to screen zearalenone (ZEN) in edible and medical coix seeds without any further correction. Then, the 122 batches of coix seeds were determined, and the positive rate was up to 97.54%. The contaminated distribution was further analyzed, and risk assessment was subsequently performed for its edible and medical purposes. The findings indicated that consumption of coix seeds with higher ZEN contamination levels may cause adverse health effects for both medical and edible consumption in the adult population; even under the condition of average contamination level, ZEN from coix seeds was the more prominent contributor to the total risk compared to other sources when used as food; thus, effective prevention and control should be an essential topic in the future.

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Microbiome-guided evaluation of Bacillus subtilis BIOUFLA2 application to reduce mycotoxins in maize kernels

et al. 2020

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Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect Fusarium verticillioides in Poultry Feed Samples

Cited by 8 publications

References 41 publications

Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia

Analysis of Toxigenic Fusarium Species Associated with Wheat Grain from Three Regions of Russia: Volga, Ural, and West Siberia

Development of a Reliable ic-ELISA with a Robust Antimatrix Interference Capability Based on QuEChERS Technology for the Rapid Detection of Zearalenone in Edible and Medical Coix Seeds and Subsequent Risk Assessments

Microbiome-guided evaluation of Bacillus subtilis BIOUFLA2 application to reduce mycotoxins in maize kernels

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