Development of ic-ELISA and an immunochromatographic strip assay for the detection of aristolochic acid Ⅰ

Li, Shaozhen; Wu, Xiaoling; Song, Shanshan; Zheng, Qinfen; Kuang, Hua

doi:10.1080/09540105.2018.1551331

Cited by 14 publications

(9 citation statements)

References 28 publications

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“…This contains target-specific antibody-reporter molecule conjugates. In cases where the conjugate pad was omitted, the antibody-reporter molecule conjugates were mixed into the sample with an appropriate amount [33,34,[45][46][47].When the liquid passes through the conjugates reservoir, the antibody-reporter molecule conjugates are slowly released. Few antibodies start to bind with the antigen and move together to the test zone.…”

Section: Competitive Formatmentioning

confidence: 99%

“…The compounds with carboxylic acid and hydroxyl functional groups are usually conjugated by a carbodiimidemediated reaction [33,34,47,[95][96][97][98][99][100][101][102], whereas compounds with active hydrogen, e.g., α-picolines, ketones, esters, and acetylenes, can be conjugated to an amine or amide group of the carrier by a Mannich reaction [103,104]. Furthermore, structure modifications of the parent compounds to add the reactive functional group to the molecule can be conducted when necessary.…”

Section: Production Of Hapten-carrier Moleculesmentioning

confidence: 99%

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Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

et al. 2022

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Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a complicated matrix is challenging. The lateral flow immunoassay (LFA) is one of the immunoassays well-known for its simplicity, portability, and rapidity. In this review, the general principle, components, format, and application of the LFA for phytoproducts are discussed.

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Section: Competitive Formatmentioning

confidence: 99%

Section: Production Of Hapten-carrier Moleculesmentioning

confidence: 99%

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

et al. 2022

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“…In addition, in 2018, China formulated the national standard of HPLC for the determination of AA‐A in cosmetics (GB/T 35949–2018), which has a detection limit of 0.3 mg/kg and a quantification limit of 1.0 mg/kg. At present, the detection methods employed for AA‐A include ultraviolet spectrophotometry, thin‐layer scanning, 22 thin‐layer ultraviolet spectrophotometry, HPLC, 23 liquid chromatography tandem‐mass spectrometry (LC/MS/MS), 24,25 capillary electrophoresis, 26 nuclear magnetic resonance, 27 and enzyme‐linked immunosorbent methods 28 . Instrumentation‐based detection methods are advantageous, owing to their high sensitivities and good reproducibilities.…”

Section: Introductionmentioning

confidence: 99%

Development of an immunochromatographic test strip for the rapid detection of aristolochic acid A in herbal medicinal materials

Chen

Huang

et al. 2021

Phytochemical Analysis

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Introduction: Plants containing aristolochic acid and its derivatives are nephrotoxic, mutagenic, and carcinogenic to humans; chronic diet poisoning caused by the aristolochic acid is the cause of endemic (Balkan) nephropathy and related cancers. Objective: To develop a colloidal gold immunochromatographic test strip (ICS) based on the competitive format for the rapid detection of aristolochic acid A (AA-A) in herbal medicinal materials. Materials and methods: For the ICS based on gold nanoparticles (AuNPs), the antigen [AA-A-bovine serum albumin (BSA)], and goat anti-mouse IgG were drawn on the nitrocellulose membrane as the test line (T line) and the control line (C line), respectively. Monoclonal antibody (MAb)-AuNP conjugates were sprayed onto the conjugate pad. The sensitivity of the ICS was 6 ng/mL, and the test was completed in 10 min. The analysis of AA-A in traditional Chinese medicine samples showed that the ICS results were in good agreement with those obtained by high-performance liquid chromatography methods. Conclusion:These results demonstrated that the ICS test could be used as a reliable, rapid, cost-effective, and convenient qualitative tool for on-site screening techniques to detect AA-A in herbal medicinal materials without any special instrumentation.

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“…Although this method showed high sensitivity and specificity, it was still limited by the fact that the polyclonal antibody had poor reproducibility and stability. Immunochromatographic assay strips have been widely employed for semiquantitative, qualitative detection, and mass sample screening due to their sensitivity and convenience Kong, Xie, Liu, Song, Kuang, Cui et al, 2017;Lei, Xu, Song, Liu, & Kuang, 2018;Li, Wu, Song, Zheng, & Kuang, 2019).…”

Section: Introductionmentioning

confidence: 99%

Development of a sandwich ELISA and immunochromatographic strip for the detection of shrimp tropomyosin

Zeng

Song

Zheng³

et al. 2019

Food and Agricultural Immunology

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Shrimp tropomyosin is one of the most important causes of shellfish allergy. The objective of this study was to develop a highly sensitive and specific immunoassay for the detection of shrimp tropomyosin in food. Ten monoclonal antibodies against shrimp tropomyosin were obtained by fusion and cell screening. We found two optimum monoclonal antibodies (mAbs); mAb 2 was used as the capture antibody, and HRP-labelled mAb 3 was used as the detection antibody. Using this pair of mAbs, a sandwich enzymelinked immunosorbent assay (ELISA) was developed with the limit of detection (LOD) of 0.45 ng/mL. Cross-reactivity with other food allergens using this method was found to be negligible. An immunochromatographic assay strip was also developed for the rapid detection of shrimp tropomyosin with the LOD of 0.5 ng/ mL. The results demonstrated that our developmental methods represent a useful tool for the detection of shrimp tropomyosin in food.

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Development of ic-ELISA and an immunochromatographic strip assay for the detection of aristolochic acid Ⅰ

Abstract: View related articles View Crossmark data Citing articles: 5 View citing articles Development of ic-ELISA and an immunochromatographic strip assay for the detection of aristolochic acid Ⅰ

Cited by 14 publications

References 28 publications

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Development of an immunochromatographic test strip for the rapid detection of aristolochic acid A in herbal medicinal materials

Development of a sandwich ELISA and immunochromatographic strip for the detection of shrimp tropomyosin

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