Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Nuntawong, Poomraphie; Putalun, Waraporn; Tanaka, Hiroyuki; Morimoto, Satoshi; Sakamoto, Seiichi

doi:10.1007/s11418-022-01605-6

Cited by 24 publications

(21 citation statements)

References 132 publications

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“…The sensitivity of competitive immunoassays can be enhanced by utilising a small amount of primary antibodies because competitive analytes at low concentrations can completely bind the primary antibodies. 27 As the amount of the antibodies coupled with AuNPs was increased, the antigen accessibility decreased because of the steric interference among antibodies. 19 In case of sandwich ICA, the use of bigger AuNPs and higher concentrations of mAb improved the sensitivity 28 because it increased the amount of AuNP-mAb that can associate with the test zone.…”

Section: Effect Of Aunp Particle Size and Mab-12g11 Amount On Icamentioning

confidence: 99%

Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross‐reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis

Chuphol

Nokkaew

Makkliang

et al. 2023

Phytochemical Analysis

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Introduction: Miroestrol (Mi) and deoxymiroestrol (Dmi) are trace, yet potent, phytooestrogens found in white Kwao Krua [Pueraria candollei var. mirifica (Airy Shaw & Suvat.) Niyomdham, PM]. However, the analysis of these substances is difficult because of complex matrix effects and their various analogues. In addition, alteration in the cross-reactivity of a gold nanoparticle (AuNP)-based immunochromatographic assay (ICA) resulting from the electrostatic adsorption between antibodies and AuNPs has not yet been evaluated.Objectives: This study aims to develop, characterise, and validate ICA with a monoclonal antibody exhibiting similar reactivity against Mi and Dmi (MD-mAb). Materials and Methods:The ICA performance was validated for cross-reactivity and performance in comparison with those of indirect competitive enzyme-linked immunosorbent assays (icELISAs) with MD-mAb and mAb exhibiting specificity against Mi (Mi-mAb).Results: The ICA showed a limit of detection (LOD) at 1 and 16 μg/mL for Mi and Dmi, respectively. The cross-reactivity of the ICA with Dmi was lower (6.25%) than that observed with the icELISA (120%). Cross-reactivity of ICA against other compounds of the PM was also correlated with those of icELISA; no false-positive/ negative results were observed. The repeatability and reproducibility of the ICA were confirmed. The results obtained using ICA in samples of PM are correlated with the concentrations determined through icELISAs. Conclusion:An ICA with MD-mAb was constructed and validated. However, direct conjugation via the electrostatic adsorption of mAb-AuNPs was expected to alter the cross-reactivity of ICA, especially that of the analyte analogue Dmi.

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Section: Effect Of Aunp Particle Size and Mab-12g11 Amount On Icamentioning

confidence: 99%

Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross‐reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis

Chuphol

Nokkaew

Makkliang

et al. 2023

Phytochemical Analysis

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“…Xia et al have previously reported that sample volume can affect flow rate and that there is an inverse relationship between the effective concentration of analyte in the sample and the square of the change in flow rate [42,43]. Consequently, a larger volume may result in a lower effective antibody concentration as the flow rate of the analyte increases [43]. Therefore, 5-10 µL is the recommended volume and 15 min is the optimal time to obtain reproducible results.…”

Section: Monitoring the Antibody Against Sars-cov-2mentioning

confidence: 99%

Smartphone- and Cloud-based Artificial Intelligence Quantitative Analysis System (SCAISY) for SARS-CoV-2-specific IgG Antibody Lateral Flow Assays

Kumar¹,

Ko²,

Chae³

et al. 2023

Preprint

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Smartphone-based point-of-care testing (POCT) is rapidly emerging as an alternative to traditional screening and laboratory testing, particularly in resource-limited settings. In this proof-of-concept study, we present a smartphone- and cloud-based artificial intelligence quantitative analysis system (SCAISY) for relative quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific IgG antibody lateral flow assays that enables rapid and accurate evaluation of test strips. By capturing an image with a smartphone camera, SCAISY quantitatively analyzes antibody levels and provides results to the user. We analyzed changes in antibody levels over time in over 248 individuals, including vaccine type, number of doses, and infection status, with a standard deviation of less than 10%. We also tracked antibody levels in six participants before and after SARS-CoV-2 infection. Finally, we examined the effects of lighting conditions, camera angle, and smartphone type to ensure consistency and reproducibility. This study suggests that SCAISY is a simple and powerful tool for real-time public health surveillance, enabling the acceleration of quantifying SARS-CoV-2-specific antibodies generated by either vaccination or infection and tracking of personal immunity levels.

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“…Both viral antigens in nasopharyngeal swabs and specific antibodies in the blood can be easily and rapidly detected. Besides large proteins, such as viral antigens and antibodies, the LFIA also qualifies for the detection of small molecules, such as phytoproducts, mycotoxins, and pesticides [ 40 , 41 , 42 , 43 ]. An LFIA for the direct detection of CHLP based on colloidal gold detection has been developed previously [ 44 ].…”

Section: Introductionmentioning

confidence: 99%

Biosensing Chlorpyrifos in Environmental Water Samples by a Newly Developed Carbon Nanoparticle-Based Indirect Lateral Flow Assay

Willemsen

Wichers

et al. 2022

Biosensors

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Pesticides are used in agriculture to prevent pests. Chlorpyrifos (CHLP) is an insecticide with potentially detrimental effects on humans, bees, and the aquatic environment. Its effects have led to a total ban by the European Union (EU), but outside the EU, CHLP is still produced and used. An indirect lateral flow immunoassay (LFIA) for the detection of CHLP was developed and integrated into a cassette to create a lateral flow device (LFD). Species-specific reporter antibodies were coupled to carbon nanoparticles to create a detector conjugate. Water samples were mixed with a specific CHLP monoclonal antibody and detector conjugate and applied to the LFD. Dose-response curves elicited the detection of low concentrations of CHLP (<1 µg/L). This sensitivity was recorded through a rapid handheld digital imaging device but also visually by naked eye. The CHLP LFD was applied to a range of European surface water samples, fortified with CHLP, revealing a sensitivity in these matrices of 2 µg/L, both by digital and visual analysis. To improve the simplicity of the CHLP LFIA, the assay reagents were dried in tubes, enabling to carry out the test by simply adding water samples and inserting the LFIA strips. This CHLP LFIA is thus suited for the on-site screening of surface waters.

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Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Cited by 24 publications

References 132 publications

Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross‐reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis

Immunochromatographic assay for miroestrol and deoxymiroestrol, its cross‐reactivity, and application in Pueraria mirifica (white Kwao Krua) analysis

Smartphone- and Cloud-based Artificial Intelligence Quantitative Analysis System (SCAISY) for SARS-CoV-2-specific IgG Antibody Lateral Flow Assays

Biosensing Chlorpyrifos in Environmental Water Samples by a Newly Developed Carbon Nanoparticle-Based Indirect Lateral Flow Assay

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