Development of<i>in vitro</i>Techniques for the Important Medicinal Plant<i>Veratrum californicum</i>

Ma, R.; Ritala, Anneli; Oksman-Caldentey, Kirsi-Marja; Rischer, Heiko

doi:10.1055/s-2006-946697

Cited by 18 publications

(9 citation statements)

References 28 publications

(40 reference statements)

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“…The enriched medium with added carbon source, optimized photoperiod or the stresses may enhance synthesis of alkaloids in culture (Rocha et al 2005, Bhat et al 2008). We suggest that certain level of differentiation may be necessary for enhanced content of secondary metabolites (see also Pande 2002, Ma et al 2006.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 92%

In vitro regeneration of Solanum nigrum with enhanced solasodine production

Bhat¹,

Mujib²,

Aslam³

et al. 2010

Biol. plant.

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High frequency of shoot formation was achieved from Solanum nigrum L. leaves on Murashige and Skoog (MS) medium without any callusing stage. Shoot forming ability was more pronounced on leaves positioned dorsally. For shoot induction, 2.0 mg dm -3 benzylaminopurine and 1.5 mg dm -3 kinetin were observed to be the most effective plant growth regulators (PGRs). The present paper also describes first successful induction of in vitro flowering in S. nigrum. The leaf derived shoots were excised and treated with various root promoting PGRs and 0.25 mg dm -3 indole-3-butyric acid produced maximum number of roots (15.2 per plant). Plants were later transplanted in field with 100 % survival. Solasodine content was higher in in vitro raised shoots and leaf derived callus, compared to ex vitro grown shoots.

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Section: ⎯⎯⎯⎯mentioning

confidence: 92%

In vitro regeneration of Solanum nigrum with enhanced solasodine production

Bhat¹,

Mujib²,

Aslam³

et al. 2010

Biol. plant.

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“…2) do not induce cyclopia [9] but may lead to other congenital birth defects and different types of toxicities [11,12]. Because of its potential medicinal use, in vitro techniques for propagating Veratrum californicum have been developed [13].…”

Section: Plants Of the Genus Veratrum And Their Teratogenic Constituentsmentioning

confidence: 99%

From Teratogens to Potential Therapeutics: Natural Inhibitors of the Hedgehog Signaling Network Come of Age

Hovhannisyan¹,

Matz²,

Gebhardt³

2009

Planta Med

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In 1963, Binns et al. [1] described the development of congenital cyclopean-type malformations in lambs following maternal ingestion of the range plant Veratrum californicum (Durand). While cattle, sheep, rat, and rabbit embryos under such conditions develop mainly cyclopia, chicken embryos preferentially develop holoprosencephaly [1-4]. Holoprosencephaly is a developmental field defect of impaired midline cleavage of the embryonic forebrain (for reviews, see [5] and [6]). However, there is a wide phenotypic spectrum depending upon the specific cause of this malformation. In its most extreme form, it is associated with cyclopia, which is characterized by one eye being located centrally over the root of the nose (l " Fig. 1). In sheep such effects occur when pregnant ewes are exposed on day 14 of gestation. The most frequent defects caused by exposure at later periods of gestation, around day 30, are limb deformities, palate defects, and severe tracheal stenosis [7]. All these defects in

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“…Metabolite production (or metabolism) is not only restricted to the specific tissues or cells, it is also modulated by different cellular and environmental factors (Pasquali et al 1992;Junaid et al 2008). From this observation it appears that the differentiation and development of the tissues are clearly the preconditions for the biosynthesis of metabolite (Pande et al 2002;Ma et al 2006). Literature suggests that reports on systemic monitoring of alliin in different stages of embryogenesis and in vitro grown plantlets are lacking.…”

Section: Resultsmentioning

confidence: 99%

Alliin production in various tissues and organs of Allium sativum grown under normal and sulphur-supplemented in vitro conditions

Nasim

Dhir

Kapoor

et al. 2010

Plant Cell Tiss Organ Cult

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Garlic extract contains alliin (S-allyl-L-cysteine sulfoxide), an organosulphur compound that contributes to its therapeutic value and pharmacological importance. Alliin production in plant organs (leaf and root), plantlet, callus (non-embryogenic and embryogenic), embryo (proliferated, matured and germinated embryos), grown under in-vitro conditions was evaluated after 8 weeks. Highest alliin content was recorded in leaves. Clove callus and germinated embryos also showed alliin production. Evaluation of alliin content of in-vitro grown tissues both in normal (control) and sulphur supplemented conditions (4, 8, 16, 32 mg l -1 ) showed that sulphur treatment at supply of 16 mg l -1 gypsum (CaSO 4 ) significantly enhanced the production of alliin content in all in-vitro grown tissues and organs. These findings suggest that production of alliin is dependent upon type and age of tissue and can be altered by growth conditions.

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Development ofin vitroTechniques for the Important Medicinal PlantVeratrum californicum

Cited by 18 publications

References 28 publications

In vitro regeneration of Solanum nigrum with enhanced solasodine production

In vitro regeneration of Solanum nigrum with enhanced solasodine production

From Teratogens to Potential Therapeutics: Natural Inhibitors of the Hedgehog Signaling Network Come of Age

Alliin production in various tissues and organs of Allium sativum grown under normal and sulphur-supplemented in vitro conditions

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