Development of HPTLC method for estimation of piperine, guggulsterone E and Z in polyherbal formulation

Vyas, J. V.; Itankar, Prakash R.; Tauqeer, Mohammad; Kelkar, Amruta; Agrawal, Mona

doi:10.1016/j.phcgj.2013.10.003

Cited by 13 publications

(6 citation statements)

References 4 publications

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“…Mean detected values of guggulsterone-E and guggulsterone-Z were 730.28 and 710.84 μg/g in UHPLC, and 692.97 and 699.87 μg/g in HPTLC, respectively, showing prominence of these two core ingredients of MYG. These cis and trans isomers (guggulsterone-E and guggulsterone-Z) have been estimated by many researchers in raw material (gum-resin, stem, leaf, and root) of Commiphora wightii [52,53] and its commercial formulations [54]. This is worth mentioning that simultaneous, rapid determination of guggulsterones and other signature components of MYG has still been underexplored.…”

Section: Comparative Quantitative Analysis Of Myg Using Uhplc and Hptlcmentioning

confidence: 99%

Standardization and validation of phytometabolites by UHPLC and high‐performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul

Balkrishna

Tomer

Joshi

et al. 2022

J of Separation Science

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MahayograjGuggul is an ancient ayurvedic medicine, prescribed for various joint disorders like arthritis, gout, and rheumatism. The present research was envisaged to develop a simple, sensitive, and comprehensive analytical method for standardization of Mahayograj Guggul. The analysis was conducted for gallic acid, protocatechuic acid, vanillic acid, cinnamic acid, piperine, guggulsterone-E, and guggulsterone-Z by high-performance thin-layer chromatography, and additionally ferulic acid, ellagic acid, and picroside I by ultra high-performance liquid chromatography. These developed methods were validated as per international guidelines, and were found linear (r 2 > 0.99), sensitive, precise (relative standard deviation < 5%), and accurate with recovery values (85-105%). The limit of detection and quantification were in the range of 0.11-23.6 and 0.33-71.51 μg/g. Gas chromatography tandem mass spectrometry was used to develop Mahayograj Guggul fingerprinting profile and to identify mid-polar or nonpolar compounds. Proximate analysis was used to ascertain the functional groups present in Mahayograj Guggul. Ultra high-performance liquid chromatography and gas chromatography tandem mass spectrometry were further employed to authenticate quality reproducibility in the active ingredients of Mahayograj Guggul in six commercial batches. Taken together, these analytical methods provide a scientific basis and reference for quality control evaluation of Mahayograj Guggul and similar traditional broad-spectrum formulations.

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Section: Comparative Quantitative Analysis Of Myg Using Uhplc and Hptlcmentioning

confidence: 99%

Standardization and validation of phytometabolites by UHPLC and high‐performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul

Balkrishna

Tomer

Joshi

et al. 2022

J of Separation Science

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“…Recently, HPLC has become a more common method for the detection of piperine, and its accuracy is better than the UV spectrophotometry method (Hirasa and Takemasa ). High‐performance thin‐layer chromatography (HPTLC) method has also been used for detection of piperine in herbal formulations (Vyas and others ). HPTLC is an enhanced form of TLC intended to increase the resolution of the compounds to be separated and can provide more accurate measurements.…”

Section: Detection Of Piperinementioning

confidence: 99%

Piperine—The Bioactive Compound of Black Pepper: From Isolation to Medicinal Formulations

Gorgani

Mohammadi

Najafpour

et al. 2016

Comp Rev Food Sci Food Safe

299

175

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Piperine is the major bio-active component of pepper, which imparts pungency and biting taste to it. This naturally occurring alkaloid has numerous demonstrated health effects and beneficial therapeutic properties; nevertheless, its biological applications are limited due to its poor solubility in aqueous environments. This emphasizes an implementation of advanced extraction approaches which could enhance the extraction yield of piperine from pepper and also the development of new formulations containing piperine to improve its in vivo bioavailability. This paper presents a review on the therapeutic and medicinal effects of piperine, its isolation from pepper fruit and the development of new formulations for its medicinal (pharmaceutical) applications. A thorough review on conventional and advanced separation techniques for the extraction of piperine from pepper is presented and an outline of the most significant conditions to improve the extraction yield is provided and discussed. Different methods used to measure and quantify the isolated piperine are also reviewed. An overview of biotechnological advancements for nanoparticle formulations of piperine or its incorporation in lipid formulations, which could enhance its bioavailability, is also presented.

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“…In addition, we have known since decades that chemical constituents as an extractable matter which obtained from the extraction process were influenced by extraction parameters, also influenced by the quality of the medicinal plant (Vyas et al, 2013). So, if the extraction process can be optimized in terms of bioactive compounds content such as anthocyanin, flavonoid, polyphenol and tannin.…”

Section: Introductionmentioning

confidence: 99%

Optimization of aqueous extraction conditions for bioactive compounds from fresh Pouzolzia zeylanica plant using response surface methodology

Nguyen¹

2020

The J. Agric. Dev.

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Response surface methodology was applied to optimize the extraction of phenolic compounds from fresh Pouzolzia zeylanica plant using hot water as a solvent. A central composite design (CCD) in form (23+star) was used to investigate the effects of two independent variables, namely, extraction temperature (70 to 90oC) and extraction time (20 to 40 min). The dependent variables were the content of anthocyanin, flavonoid, polyphenol, tannin and total soluble solids of extracted solution. A second-order polynomial model was used for predicting the response. The results showed that the optimal extraction process was obtained at 84.4oC for 31.7 min. The experimental values agreed with predicted within a 95% confidence interval. Consequently, the contents of anthocyanin, flavonoid, polyphenol and tannin were 38.66 mgCE/100 g, 3.01 mgQE/g, 5.17 mgGAE/g, 4.07 mgTAE/g fresh weight, and total soluble solids content was 0.73%, respectively.

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Development of HPTLC method for estimation of piperine, guggulsterone E and Z in polyherbal formulation

Cited by 13 publications

References 4 publications

Standardization and validation of phytometabolites by UHPLC and high‐performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul

Standardization and validation of phytometabolites by UHPLC and high‐performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul

Piperine—The Bioactive Compound of Black Pepper: From Isolation to Medicinal Formulations

Optimization of aqueous extraction conditions for bioactive compounds from fresh Pouzolzia zeylanica plant using response surface methodology

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