Development of highly polymorphic tetranucleotide microsatellite markers in Austrocedrus chilensis

Abstract

An enriched genomic library was constructed and 9 novel polymorphic tetranucleotide microsatellite markers developed for Austrocedrus chilensis, the most economically important native conifer in the Andean Patagonian region. Polymorphism was investigated for these markers in 48 individuals from two populations. Numbers of alleles ranged from 3 to 19 and levels of observed heterozygosity among the 9 loci ranged from 0.32 to 0.95. No locus combinations exhibited linkage disequilibrium. These polymorphic markers will be useful tools for the study of demography and gene flow and more in general for population and conservation genetics of this species.

Extensive pollen flow in a natural fragmented population of Patagonian cypress Austrocedrus chilensis

“…We genotyped all individuals at five highly polymorphic unlinked tetranucleotide microsatellite loci (Achi3, Achi4, Achi5, Achi6, and Achi7) (Arana et al 2008). PCR amplification was performed in a total volume of 15 μL, containing about 20 ng of total DNA, 0.15 % bovine seroalbumin, 1× GoTaq PCR buffer (Promega), 200 μM each dNTP (GE Healthcare), 1 U Taq polymerase (GoTaq, Promega), and 0.2 μM each primer.…”

Anatomía comparada de la madera de Cupressaceae y su correspondencia con los estudios de filogenia

“…. Las poblaciones en Argentina ocupan casi 132.000 ha, cubriendo uno de los mayores gradientes de precipitación del mundo (Arana et al 2008). En Chile sus masas no presentan más de 41.000 ha.…”