Development of glycated peptide enzyme sensor based flow injection analysis system for haemoglobin A1c monitoring using quasi-direct electron transfer type engineered fructosyl peptide oxidase

Hatada, Mika; Saito, Satomi; Yonehara, Satoshi; Tsugawa, Wakako; Asano, Ryutaro; Sode, Koji

doi:10.1016/j.bios.2021.112984

Cited by 16 publications

(4 citation statements)

References 36 publications

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“…The previous report was carried out using aCSF by adding human serum albumin to mimic real CSF, revealing that arPES-modified biosensing molecule works within a relevant biological fluid. Additionally, the detection of digested haemoglobin A1c using arPES-modified enzyme was also reported [15]. These reports indicate the feasibility of arPES application in the biological samples within a complex sample matrix for E-ABs.…”

Section: Discussionmentioning

confidence: 70%

“…The modification of redox enzymes by arPES can be achieved by a rapid and single-step conjugation, through the reaction of the N-hydroxysuccinimide ester moiety of arPES with solvent-accessible primary amine groups (e.g., Lysine residues). Thus, conjugation of arPES enabled the preparation of quasi-direct electron transfer type enzymes that do not require a mediator in solution [12][13][14][15]. Recently, we also reported the construction of a continuous glutamine sensor using arPES-modified glutamine binding protein, which undergoes a conformation change upon interaction with glutamine [16].…”

Section: Introductionmentioning

confidence: 99%

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An Amine-Reactive Phenazine Ethosulfate (arPES)—A Novel Redox Probe for Electrochemical Aptamer-Based Sensor

Nagata

Lee

Henley

et al. 2022

Sensors

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Electrochemical aptamer-based biosensors (E-ABs) are attractive candidates for use in biomarker detection systems due to their sensitivity, rapid response, and design flexibility. There are only several redox probes that were employed previously for this application, and a combination of redox probes affords some advantages in target detection. Thus, it would be advantageous to study new redox probes in an E-AB system. In this study, we report the use of amine-reactive phenazine ethosulfate (arPES) for E-AB through its conjugation to the terminus of thrombin-binding aptamer. The constructed E-AB can detect thrombin by square-wave voltammetry (SWV), showing peak current at −0.15 V vs. Ag/AgCl at pH 7, which differs from redox probes used previously for E-ABs. We also compared the characteristics of PES as a redox probe for E-AB to methylene blue (MB), which is widely used. arPES showed stable signal at physiological pH. Moreover, the pH profile of arPES modified thrombin-binding aptamer revealed the potential application of arPES for a simultaneous multianalyte detection system. This could be achieved using different aptamers with several redox probes in tandem that harbor various electrochemical peak potentials. Our findings present a great opportunity to improve the current standard of biological fluid monitoring using E-AB.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

An Amine-Reactive Phenazine Ethosulfate (arPES)—A Novel Redox Probe for Electrochemical Aptamer-Based Sensor

Nagata

Lee

Henley

et al. 2022

Sensors

Self Cite

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“…In addition, to prove that the FPOX-modified electrode can specifically measure FVH, significant changes in electron transfer resistance were observed after incubation of the FPOX-modified electrode with FVH, but there was no response in the control group, indicating the specific measurement of FVH. Hatada et al replaced Arg414 with Lys to form the PnFPOX (FPOX from Phaeosphaeria nodorum) N56A/R414K mutant and modified PnFPOX near FAD with amine-reactive phenazine ethosulfate (arPES), which showed quasi-direct electron transferability [106]. This electrode was combined with an enzyme flow injection analysis (FIA) system.…”

Section: Fpox Typementioning

confidence: 99%

A Review of Electrochemical Sensors for the Detection of Glycated Hemoglobin

Zhan

Zhao

et al. 2022

Biosensors

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Glycated hemoglobin (HbA1c) is the gold standard for measuring glucose levels in the diagnosis of diabetes due to the excellent stability and reliability of this biomarker. HbA1c is a stable glycated protein formed by the reaction of glucose with hemoglobin (Hb) in red blood cells, which reflects average glucose levels over a period of two to three months without suffering from the disturbance of the outside environment. A number of simple, high-efficiency, and sensitive electrochemical sensors have been developed for the detection of HbA1c. This review aims to highlight current methods and trends in electrochemistry for HbA1c monitoring. The target analytes of electrochemical HbA1c sensors are usually HbA1c or fructosyl valine/fructosyl valine histidine (FV/FVH, the hydrolyzed product of HbA1c). When HbA1c is the target analyte, a sensor works to selectively bind to specific HbA1c regions and then determines the concentration of HbA1c through the quantitative transformation of weak electrical signals such as current, potential, and impedance. When FV/FVH is the target analyte, a sensor is used to indirectly determine HbA1c by detecting FV/FVH when it is hydrolyzed by fructosyl amino acid oxidase (FAO), fructosyl peptide oxidase (FPOX), or a molecularly imprinted catalyst (MIC). Then, a current proportional to the concentration of HbA1c can be produced. In this paper, we review a variety of representative electrochemical HbA1c sensors developed in recent years and elaborate on their operational principles, performance, and promising future clinical applications.

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“…FV occurs as an N-terminal unit of human HbA1c inside β-subunits (fructosyl-Val-His-Leu-Thr…) 8,9 and typically serves as a target test molecular moiety and chemical model to mimic the diagnosis of type 2 diabetes mellitus. 1,10,11 To date, an overwhelming majority of FV and related fructosyl amino acid detection methods are based on chromatography separation-mass spectrometry, 10,12 mutant proteins, 13 electrochemical biosensors, 2,9,14–18 and Pt/Au-amperometric nanomaterials 5,9,19,20 that contain the fructosyl amino-acid oxidase. These enzymatic assay systems still suffer from some drawbacks such as multiple steps for their construction, long analysis times and the requirement for specialized labs.…”

Section: Introductionmentioning

confidence: 99%

Molecular two-point recognition of fructosyl valine and fructosyl glycyl histidine in water by fluorescent Zn(ii)-terpyridine complexes bearing boronic acids

Salomón-Flores,

Valdes-García,

Viviano-Posadas

et al. 2024

Dalton Trans.

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Development of glycated peptide enzyme sensor based flow injection analysis system for haemoglobin A1c monitoring using quasi-direct electron transfer type engineered fructosyl peptide oxidase

Cited by 16 publications

References 36 publications

An Amine-Reactive Phenazine Ethosulfate (arPES)—A Novel Redox Probe for Electrochemical Aptamer-Based Sensor

An Amine-Reactive Phenazine Ethosulfate (arPES)—A Novel Redox Probe for Electrochemical Aptamer-Based Sensor

A Review of Electrochemical Sensors for the Detection of Glycated Hemoglobin

Molecular two-point recognition of fructosyl valine and fructosyl glycyl histidine in water by fluorescent Zn(ii)-terpyridine complexes bearing boronic acids

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