Development of glucose biosensor based on ZnO nanoparticles film and glucose oxidase-immobilized eggshell membrane

Aini, Bohari Noor; Siddiquee, Shafiquzzaman; Ampon, Kamaruzaman Hj.; Rodrigues, Kenneth Francis; Saallah, Suryani

doi:10.1016/j.sbsr.2015.03.004

Cited by 54 publications

(38 citation statements)

References 37 publications

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“…To enhance the enzymatic activity, lifetime, and stability of GOx, various new nanoscale materials and technologies have been used to immobilize enzymes. Compared with free enzymes, the immobilized GOx performs much better in a variety of batch or continuous processes, such as rapid termination of reactions, controlled product formation, ease of enzyme removal from the reaction mixture and adaptability to various engineering designs [2][3][4][5]. Immobilized GOx on various nanoparticles, including gold [2], ZnO [3,4], NiO and silica [5][6][7], has been reported in the development of glucose biosensor.…”

Section: Introductionmentioning

confidence: 99%

Rational surface silane modification for immobilizing glucose oxidase

Tian

Guo

Zhang

et al. 2016

International Journal of Biological Macromolecules

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Section: Introductionmentioning

confidence: 99%

Rational surface silane modification for immobilizing glucose oxidase

Tian

Guo

Zhang

et al. 2016

International Journal of Biological Macromolecules

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“…Glucose biosensor: A novel electrochemical glucose biosensor was developed by depositing an Ionic Liquid (IL) (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate; [EMIM] [Otf]), ZnO nanoparticles (ZnO NPs) and Eggshell Membrane (ESM) on a modified Glassy Carbon Electrode (GCE) for determination of glucose [22]. Enzymatic glucose biosensor was fabricated with the aid of zinc oxide (ZnO) porous nanostructure on FTO glass substrate by simple drop casting technique.…”

Section: Uv-sensorsmentioning

confidence: 99%

A Review on Zinc Sulphide Nanoparticles: From Synthesis, Properties to Applications

2016

J Bioelectron Nanotechnol

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In this paper the synthesis of zinc sulfide nanoparticles by different routes has been discussed. The emergence of nanotechnology and the synthesis of nanomaterials have revolutionized the field of science and technology. ZnS tends to show the most promising nanoscale morphologies among the inorganic semiconductors thus possessing versatile novel properties and applications. These applications include their use in Field Emitting Diodes (FET), electroluminescence, sensors (UV-light sensors, gas sensors, biosensors), flat panel displays. This article begins with the brief introduction to ZnS its structure, description of physical, chemical and electronic properties. Further followed by the recent experiments conducted in order to improve its nanoscale properties and thus idealizing its applications.

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“…This can lead to the ineffective performance of enzymebased glucose biosensors. To overcome these obstacles, creative approaches have been applied to maintain and improve the biological activity of GOx [30]. One attractive strategy is the use of nanomaterials, a conducting polymer and a polymer membrane which can efficiently enhance electron transfer and enzyme loading.…”

Section: Introductionmentioning

confidence: 99%

Enhancing a Novel Robust Multicomposite Materials Platform for Glucose Biosensors

et al. 2019

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An effective, stable enzymatic glucose biosensor was fabricated on a glassy carbon electrode (GCE) surface using simple multicomposite materials (MCM): a solution of prepared poly(diallyldimethylammonium chloride)‐capped gold nanoparticles‐nickel ferrite particles‐carbon nanotubes‐chitosan (PDDA‐AuNPs‐NiFe2O4‐CNTs‐CHIT), electropolymerization of poly(o‐phenylenediamine) (PoPD) and immobilization of glucose oxidase (GOx). Biocompatibility and synergy of the MCM enhanced the immobilization and the reaction of GOx and as well as the electron transfer from an oxidation reaction of hydrogen peroxide in the system. The NiFe2O4 was synthesized by co‐precipitation and calcined at 700 °C. Characterization was carried out by field emission scanning electron microscopy (FE‐SEM), energy‐dispersive X‐ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR) and X‐ray diffraction (XRD) which presented both tetrahedral and octahedral metal stretching with a cubic NiFe2O4 crystal phase. The GOx/PoPD/MCM/GCE yielded a 0.77 s−1 charge transfer rate constant (Ks), a 2.28×10−6 cm2 s−1 diffusion coefficient value (D), a 0.21 mm2 electroactive surface area (Ae) and a 1.93×10−8 mol cm−2 surface concentration (γ ) as determined by cyclic voltammetry. The modified electrode showed a durable operation time (n=97, more than 50 % I), repeatability (%RSD=0.38, n=10), reproducibility (%RSD=1.60, n=10), high sensitivity (853.07 μA mM−1 cm−2), selectivity without effects of electroactive species (aspirin, uric acid, caffeine, cholesterol, ascorbic acid and dopamine) and two linear ranges from 0.5 to 10 μM (R2=0.998) and 10 to 15,000 μM (R2=0.991) with a low detection limit (0.35 μM, S/N=3). Its Michaelis‐Menten constant (Km) was calculated as 93.51 μM with 46.30 μA maximum current (Imax). This proposed simple method was successfully applied for glucose determination in human blood samples.

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Development of glucose biosensor based on ZnO nanoparticles film and glucose oxidase-immobilized eggshell membrane

Cited by 54 publications

References 37 publications

Rational surface silane modification for immobilizing glucose oxidase

Rational surface silane modification for immobilizing glucose oxidase

A Review on Zinc Sulphide Nanoparticles: From Synthesis, Properties to Applications

Enhancing a Novel Robust Multicomposite Materials Platform for Glucose Biosensors

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