Development of gelatin methacrylate (GelMa) hydrogels for versatile intracavitary applications

Kulkarni, Nishant S.; Chauhan, Gautam; Goyal, Mimansa; Sarvepalli, Sruthi; Gupta, Vivek

doi:10.1039/d2bm00022a

Cited by 22 publications

(17 citation statements)

References 57 publications

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“…The methylene peak of lysine is at 2.9 ppm, and then, the substitution rate is calculated based on 7.2 ppm phenylalanine. These results indicate that gelatin can be modified with methacryloyl substitutes at specific amino acid residues, allowing for the formation of covalent crosslinks with the photoinitiator . The degree of modification is quantified as the substitution rate, which represents the percentage of amino acids in gelatin that have been successfully replaced with methacryloyl groups.…”

Section: Resultsmentioning

confidence: 95%

“…These results indicate that gelatin can be modified with methacryloyl substitutes at specific amino acid residues, allowing for the formation of covalent crosslinks with the photoinitiator. 27 The degree of modification is quantified as the substitution rate, which represents the percentage of amino acids in gelatin that have been successfully replaced with methacryloyl groups. The substitution rate is a crucial parameter as it directly influences the properties of GelMA, including its mechanical characteristics, 28 swelling behavior, and pore formation.…”

Section: Materials Characterizationmentioning

confidence: 99%

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Photocrosslinked Gelatin Methacryloyl (GelMA)/Hyaluronic Acid Methacryloyl (HAMA) Composite Scaffold Using Anthocyanidin as a Photoinitiator for Bone Tissue Regeneration

Lin

Nixon

Wang

et al. 2023

ACS Appl. Polym. Mater.

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Gelatin and hyaluronic acid are natural biopolymers that are important components of soft tissues in the human body, and both have been used in several biomedical applications. To achieve controllable mechanical and degradation properties of gelatin and hyaluronic acid, methacrylic acid has been used to modify photocrosslinkable gelatin methacryloyl (GelMA) and hyaluronic acid methacryloyl (HAMA). Irgacure 2959 (I2959) and anthocyanidin have been used as photoinitiators for comparison. Finally, the photocrosslinked GelMA/HAMA composite scaffold has been prepared for bone tissue regeneration. The composite scaffolds were prepared using mold casting and photocrosslinking techniques. Specific ratios of GelMA and HAMA reagents were mixed properly with I2959 or anthocyanidin photoinitiators. Samples were examined with chemical and material, in vitro, and in vivo evaluations. Furthermore, two photoinitiators, I2959 and anthocyanidin, can crosslink the GelMA/HAMA composite scaffold. A 1% HAMA incorporation can significantly decrease the degradation of the GelMA/HAMA scaffold compared with GelMA only. Also, HAMA participation can improve cell viability and promote bone-related cell differentiation and tissue regeneration (bone formation) without any apparent adverse reactions in vivo.

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Section: Resultsmentioning

confidence: 95%

Section: Materials Characterizationmentioning

confidence: 99%

Photocrosslinked Gelatin Methacryloyl (GelMA)/Hyaluronic Acid Methacryloyl (HAMA) Composite Scaffold Using Anthocyanidin as a Photoinitiator for Bone Tissue Regeneration

Lin

Nixon

Wang

et al. 2023

ACS Appl. Polym. Mater.

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“…They found that the cells remained viable after contact with the tested material. Kulkarni et al tested the biocompatibility of a GelMA hydrogel through an MTT assay, and the cell viability found was above 80% [ 34 , 35 ].…”

Section: Resultsmentioning

confidence: 99%

Hydrogel Based on Nanoclay and Gelatin Methacrylate Polymeric Matrix as a Potential Osteogenic Application

Andrade

Soares

Cardoso

et al. 2023

JFB

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A nanocomposite hydrogel has potentially applicability in the induction of osteogenesis. The hydrogel was synthesized using 1% gelatin methacrylate (GelMA), a biodegradable and bioactive polymer containing the structure of gelatin, denatured collagen derived from the extracellular bone matrix, and 6% laponite (Lap), a synthetic phyllosilicate of nanosized particles. Initially, 0.6 g of Lap was added to deionized water, and then a solution of GelMA/Igarcure was added under stirring and UV light for crosslinking. The spectra in the Fourier-transform infrared region showed bands that indicate the interaction between gelatin and methacrylate anhydride. X-ray diffraction patterns confirmed the presence of Lap and GelMA in the hydrogel. The thermogravimetric analysis suggested an increase in the thermal stability of the hydrogel with the presence of clay mineral. Rheological analysis showed that the hydrogel had a viscosity that allowed its injectability. The hydrogel did not show acute toxicity at any of the concentrations tested according to the Artemia salina lethality test. It showed cell viability more significant than 80% in the MTT test, which makes it suitable for in vivo osteogenic induction tests. The cell differentiation test showed the differentiation of stem cells into osteogenic cells. It indicates a material with the potential for osteogenic induction and possible application in bone tissue engineering.

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“…The cells were then treated with the plain drug and the prepared nanoemulsion at a concentration ranging from 0.09 to 12.5 µM along with the no treatment media as control, followed by incubation over a period of 72 h at 37 °C/5% CO 2 . The cellular viability was then evaluated using an MTT assay using a Tecan Spark 10M Plate reader (Tecan, Männedorf, Switzerland), following protocols established earlier by our group [ 36 ]. All cytotoxicity studies were repeated at least three times with n = 6 each time.…”

Section: Methodsmentioning

confidence: 99%

Scalable Production and In Vitro Efficacy of Inhaled Erlotinib Nanoemulsion for Enhanced Efficacy in Non-Small Cell Lung Cancer (NSCLC)

et al. 2023

Self Cite

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Non-small cell lung cancer (NSCLC) is a global concern as one of the leading causes of cancer deaths. The treatment options for NSCLC are limited to systemic chemotherapy, administered either orally or intravenously, with no local chemotherapies to target NSCLC. In this study, we have prepared nanoemulsions of tyrosine kinase inhibitor (TKI), erlotinib, using the single step, continuous manufacturing, and easily scalable hot melt extrusion (HME) technique without additional size reduction step. The formulated nanoemulsions were optimized and evaluated for their physiochemical properties, in vitro aerosol deposition behavior, and therapeutic activity against NSCLC cell lines both in vitro and ex vivo. The optimized nanoemulsion showed suitable aerosolization characteristics for deep lung deposition. The in vitro anti-cancer activity was tested against the NSCLC A549 cell line which exhibited 2.8-fold lower IC50 for erlotinib-loaded nanoemulsion, as compared to erlotinib-free solution. Furthermore, ex vivo studies using a 3D spheroid model also revealed higher efficacy of erlotinib-loaded nanoemulsion against NSCLC. Hence, inhalable nanoemulsion can be considered as a potential therapeutic approach for the local lung delivery of erlotinib to NSCLC.

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Development of gelatin methacrylate (GelMa) hydrogels for versatile intracavitary applications

Abstract: Applicability of hydrogels as drug delivery systems is on the rise due to their highly tunable degree of polymeric crosslinking to attain varying rates of payload release. Sustaining the release...

Cited by 22 publications

References 57 publications

Photocrosslinked Gelatin Methacryloyl (GelMA)/Hyaluronic Acid Methacryloyl (HAMA) Composite Scaffold Using Anthocyanidin as a Photoinitiator for Bone Tissue Regeneration

Photocrosslinked Gelatin Methacryloyl (GelMA)/Hyaluronic Acid Methacryloyl (HAMA) Composite Scaffold Using Anthocyanidin as a Photoinitiator for Bone Tissue Regeneration

Hydrogel Based on Nanoclay and Gelatin Methacrylate Polymeric Matrix as a Potential Osteogenic Application

Scalable Production and In Vitro Efficacy of Inhaled Erlotinib Nanoemulsion for Enhanced Efficacy in Non-Small Cell Lung Cancer (NSCLC)

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