Development of Functional Cosmetic Material Using a Combination of Hippophae rhamnoides Fruit, Rubus fruticosus Leaf and Perillae folium Leaf Extracts

Abstract

Purpose: This study aimed to determine the optimal blending ratio of extracts of Hippophae rhamnoides (H. rhamnoides) fruit, Rubus fruticosus (R. fruticosus) leaf, and Perillae folium (P. folium) leaf as a potential functional material for antiwrinkle, antioxidant, anti-inflammatory, and whitening properties. Methods: We used the design of experiment (DOE) efficacy method to identify the optimal blend ratio for a natural extract complex. In addition, we tested the efficacy of the extract complex and the individual extracts by experiments, including elastase inhibition assay, NO inhibition assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay, tyrosinase inhibition assay, and ROS inhibition assay. Results: DOE methods using natural extracts showed that the most efficacious ratio of H. rhamnoides fruit: R. fruticosus leaf: P. folium leaf was 0.25:0.7:0.05. At this ratio, the elastase inhibitory effect, an indicator of wrinkle improvement, was 28% compared to the control. The antioxidant activity was 71.8% higher than the control. Additionally, NO formation was significantly suppressed by the extract complex. Tyrosinase inhibitory activity, a whitening index, showed 24.4% improvement over the control. Finally, hydrogen peroxide-induced cytotoxicity was significantly inhibited following treatment with the extract complex. Conclusion: The natural extract complex of H. rhamnoides, R. Fruticosus, and P. folium was found to have beneficial wrinkle improvement, antioxidant, anti-inflammatory, and whitening effects, and has potential as a functional cosmetic material.

Biological Effects of Yeast-fermented Plant Root Extract Mixture on Skin Cells

“…The synergic effect of a mixture of plant extract and the fermented mixture of plant extract have been reported (Kim et al, 2019;Um et al, 2017). Five roots were selected to be mixed and extracted (MC).…”

“…The benefits of mixed plant extracts and the mixed extracts have been reported to be better than those of individual extracts (Choi, 2022;Kim et al, 2019;Lee et al, 2021). By fermentation, certain common substrate components are degraded or transformed into compatible components.…”

Expression Changes of Genes Related to Germination Based on EST Database under Priming Treatment by Gibberellic Acid in Perilla frutescens (Korean Perilla)

“…Recently, consumption of perilla has increased significantly in Korea; more than 60% of the total unsaturated fatty acids (FAs) in perilla seeds comprises αlinolenic acid (Ichikawa 2006), an essential FA required for human growth and development, in addition to its known major role in preventing and treating blood vessel diseases (Shahidi and Miraliakbari 2005). Many flavonoids, sterols, terpenoids and phenolic acids have been extracted from seeds of Korean perilla and studied, with several studies reporting on the importance of flavonoids and phenolic compounds in relation to biological activity (Ozturk et al 2010;Kim et al 2019).…”