Development of food grade media for the preparation of Lactobacillus plantarum starter culture

Sawatari, Yuki; Hirano, Tomomi; Yokota, Atsushi

doi:10.2323/jgam.52.349

Cited by 20 publications

(16 citation statements)

References 15 publications

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“…Sodium acetate and ammonium citrate play important buffering roles by preventing acidification of the culture medium. 29) The omission of sodium acetate and/or ammonium citrate might lower the growth of Lactobacillus strains due to fast reduction in the medium pH. A high BC at the optimal pH for microbial growth appears to be favorable to bio-transformation.…”

Section: Resultsmentioning

confidence: 99%

Sweet Potatoes as a Basic Component in Developing a Medium for the Cultivation of Lactobacilli

Hayek

Shahbazi

Awaisheh

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Section: Resultsmentioning

confidence: 99%

Sweet Potatoes as a Basic Component in Developing a Medium for the Cultivation of Lactobacilli

Hayek

Shahbazi

Awaisheh

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…The strain was positive for its β-glucosidase activity and was responsible for the hydrolysis of PPD-type major ginsenosides (PPD-mix ginsenosides (Rb1 and Rd)) into minor ginsenosides Rg3(S). The PPD-mix-type major ginsenosides, which were extracted from Korean red ginseng, were dissolved in MRS and three different food-grade media (i.e., Basel Food-Grade (BFG), Food-Grade medium I (FG-I) and Food-Grade medium II (FG-II)) [21], with certain modifications, as presented in Table S2. The PPD-mix solutions were sterilized using 0.20 µm ADVANTEC filter paper and L. ginsenosidimutans was inoculated in the PPD-mix solutions (PPD-mix in MRS and three food-grade media).…”

Section: Strain Isolation and Fermentation Of Ppd-mix-type Major Ginsmentioning

confidence: 99%

Exploration and Characterization of Novel Glycoside Hydrolases from the Whole Genome of Lactobacillus ginsenosidimutans and Enriched Production of Minor Ginsenoside Rg3(S) by a Recombinant Enzymatic Process

et al. 2020

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Background: Several studies have reported that ginsenoside Rg3(S) is effective in treating metastatic diseases, obesity, and various cancers, however, its presence in white ginseng cannot be estimated, and only a limited amount is present in red ginseng. Therefore, the use of recombinant glycosidases from a Generally Recognized As Safe (GRAS) host strain is a promising approach to enhance production of Rg3(S), which may improve nutritional activity, human health, and quality of life. Method: Lactobacillus ginsenosidimutans EMML 3041T, which was isolated from Korean fermented pickle (kimchi), presents ginsenoside-converting abilities. The strain was used to enrich the production of Rg3(S) by fermenting protopanaxadiol (PPD)-mix-type major ginsenosides (Rb1, Rb2, Rc, and Rd) in four different types of food-grade media (1, MRS; 2, Basel Food-Grade medium; 3, Basel Food-Grade medium-I, and 4, Basel Food-Grade medium-II). Due to its tendency to produce Rg3(S), the presence of glycoside hydrolase in Lactobacillus ginsenosidimutans was proposed, the whole genome was sequenced, and the probable glycoside hydrolase gene for ginsenoside conversion was cloned. Results: The L. ginsenosidimutans EMML 3041T strain was whole genome sequenced to identify the target genes. After genome sequencing, 12 sets of glycoside hydrolases were identified, of which seven sets (α,β-glucosidase and α,β-galactosidase) were cloned in Escherichia coli BL21 (DE3) using the pGEX4T-1 vector system. Among the sets of clones, only one clone (BglL.gin-952) showed ginsenoside-transforming abilities. The recombinant BglL.gin-952 comprised 952 amino acid residues and belonged to glycoside hydrolase family 3. The enzyme exhibited optimal activity at 55 °C and a pH of 7.5 and showed a promising conversion ability of major ginsenoside Rb1→Rd→Rg3(S). The recombinant enzyme (GST-BglL.gin-952) was used to mass produce Rg3(S) from major ginsenoside Rb1. Scale-up of production using 50 g of Rb1 resulted in 30 g of Rg3(S) with 74.3% chromatography purity. Conclusion: Our preliminary data demonstrated that this enzyme would be beneficial in the preparation of pharmacologically active minor ginsenoside Rg3(S) in the functional food and pharmaceutical industries.

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“…lactis exhibit wider requirements than L. plantarum, L. pentosusand L. curvatus [34,48]. Strains belong to L. plantarum, will require nutritionally simpler medium [49]. Therefore, defects in amino acid biosynthetic capacity in L. plantarum, with big genome size, are less than those in L. johnsonii, with small genome size [50].…”

Section: Amino Acids and Peptidesmentioning

confidence: 99%

“…bulgaricus, and L. delbrueckii subsp. lactis could notgrew [49]. Thus replacing beef extract, yeast extract, and peptone with other nitrogen sources or even with each other may negatively affect the growth.…”

Section: Amino Acids and Peptidesmentioning

confidence: 99%

Current Limitations and Challenges with Lactic Acid Bacteria: A Review

Hayek¹,

Ibrahim²

2013

FNS

162

126

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Lactic acid bacteria (LAB) play a critical role in food, agricultural, and clinical applications. The fast growing characteristics of LAB and their metabolic activity have been the key in most applications including food production, agricultural industry, and probiotics. However, the biochemical and biophysical environments have significant effect on the growth and metabolic activity of LAB. While the biochemical conditions are most likely established, controlling and optimizing of biochemical conditions have many limitations and challenges. In addition to selecting the right strain, desirable metabolic processes required optimizing and controlling the available nutrients including sugars, peptides, free amino acids, minerals, and vitamins in addition to buffering agents. Thus, much of research was conducted to understand the impact of available nutrients on the growth and metabolic activities of LAB. However, only a few nutritional parameters could be controlled at a time while holding other parameters constant. The nutritional parameters may also interact with each other resulting in faulty results. Characteristics of LAB such as fastidiousness in their nutritional requirements, ability to produce acid and antimicrobial compounds, and variations in the nutritional requirements among strains have added additional limitations and challenges in this regard. Thus, chemically defined media (CDM) were suggested to deal with different limitations and challenges. However, due to differences in growth conditions, results obtained in CDM may face some obstacles when it comes to industrial applications. Thus, this paper aimed to review the recent data in regard to the role of the nutritional requirements of LAB in optimizing and controlling metabolic activities and to discuss the associated limitations and challenges.

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Development of food grade media for the preparation of Lactobacillus plantarum starter culture

Cited by 20 publications

References 15 publications

Sweet Potatoes as a Basic Component in Developing a Medium for the Cultivation of Lactobacilli

Sweet Potatoes as a Basic Component in Developing a Medium for the Cultivation of Lactobacilli

Exploration and Characterization of Novel Glycoside Hydrolases from the Whole Genome of Lactobacillus ginsenosidimutans and Enriched Production of Minor Ginsenoside Rg3(S) by a Recombinant Enzymatic Process

Current Limitations and Challenges with Lactic Acid Bacteria: A Review

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