Development of extractive electrospray ionization ion trap mass spectrometry for in vivo breath analysis

Ding, Jianhua; Yang, Shiliang; Liang, Dapeng; Chen, Huanwen; Wu, Zhuanzhang; Zhang, Lili; Ren, Yaxuan

doi:10.1039/b821497b

Cited by 55 publications

(51 citation statements)

References 65 publications

(127 reference statements)

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“…26,27 The replicates of S. aureus are clustered at the largest distance from the rest of the bacterial strains; S. haemolyticus are distant from S. aureus with highest distinction; S. hominis and S. epidermidis are close to each other but the boundaries are clear (detailed in Fig. 5).…”

Section: Resultsmentioning

confidence: 99%

Fast detection of volatile organic compounds from Staphylococcal blood cultures by CDI-MS

Hang

Chingin²,

Liang³

et al. 2017

RSC Adv.

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Rapid recognition of Staphylococcal bacteremia in humans is a serious challenge in clinical research. Here we report on direct analysis of molecular volatile organic compounds (VOCs) of blood cultures emitted by 35 patients with a suspicion of Staphylococcal bacteremia and 31 patients positively diagnosed with Staphylococcal bacteremia were fingerprinted by atmospheric corona discharge ionization mass spectrometry (CDI-MS) for the presence of four common Staphylococci (Staphylococcus aureus, S.hominis, S. haemolyticus, and S. epidermidis) which statistically account for ca. 90% of cases of Staphylococcal bacteremia in humans. All the infected blood cultures revealed characteristic and clearly distinct MS patterns specific to the presence of one of the four Staphylococci after an incubation time of 3-16 h. Technical replicates were incubated over 2-3 days for reference diagnosis using current conventional bacteremia detection. The results of our 6 month hospital study confirm peaks at m/z 87 (isobutyric acid) and m/z 101 (isovaleric acid) signals were specifically present in Staphylococci, and indicate that high suitability of CDI-MS for rapid, reliable and cost-efficient diagnosis of Staphylococcal bacteremia in humans.

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Section: Resultsmentioning

confidence: 99%

Fast detection of volatile organic compounds from Staphylococcal blood cultures by CDI-MS

Hang

Chingin²,

Liang³

et al. 2017

RSC Adv.

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“…Another advantage is that the solvents in the charged ESI spray can be tuned to selectively extract the analytes, which are needed for the MS analysis, in complex matrices [9]. These advantages render EESI an ideal secondary ionization method for the analysis of gaseous volatile, semi-volatile, and even nonvolatile substances in various complex matrices such as exhaled breath [10,11], milk [12,13], and with viscous liquids [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

On the mechanism of extractive electrospray ionization (EESI) in the dual-spray configuration

et al. 2011

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Dual-spray extractive electrospray ionization (EESI) mass spectrometry as a versatile analytical technique has attracted much interest due to its advantages over conventional electrospray ionization (ESI). The crucial difference between EESI and ESI is that in the EESI process, the analytes are introduced in nebulized form via a neutral spray and ionized by collisions with the charged droplets from an ESI source formed by spraying pure solvent. However, the mechanism of the droplet-droplet interactions in the EESI process is still not well understood. For example, it is unclear which type of droplet-droplet interaction is dominant: bounce, coalescence, disruption, or fragmentation? In this work, droplet-droplet interaction was investigated in detail based on a theoretical model. Phase Doppler anemometry (PDA) was employed to investigate the droplet behavior in the EESI plume and provide the experimental data (droplet size and velocity) necessary for theoretical analysis. Furthermore, numerical simulations were performed to clarify the influence of the sheath gas flow on the EESI process. No coalescence between the droplets in the ESI spray and the droplets in the sample spray was observed using various geometries and sample flow rates. Theoretical analysis, together with the PDA results, suggests that droplet fragmentation may be the dominant type of droplet-droplet interaction in the EESI. The interaction time between the ESI droplet and the sample droplet was estimated to be <5 μs. This work gives a clear picture of droplet-droplet interactions in the dual-spray EESI process and detailed information for the optimization of this method for future applications that require higher sensitivity.

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“…EESI-MS has been successfully used to study a number of diverse systems including gases and particles from olive oil, aerosolized drugs, human breath, and beer. [59][60][61][62][63][64][65][66][67][68] We report here the first analysis of SOA using EESI-MS. Of particular importance is the fact that the particles are directly sampled from air with no prior collection onto a substrate. Our studies focus on SOA generated from the reaction of a-pinene with ozone because we have extensive data on the SOA from this reaction using a variety of techniques.…”

Section: Introductionmentioning

confidence: 99%

Analysis of secondary organic aerosols in air using extractive electrospray ionization mass spectrometry (EESI-MS)

et al. 2012

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Extractive electrospray ionization mass spectrometry (EESI-MS) has been shown, in other laboratories, to be a useful technique for the analysis of aerosols from a variety of sources. EESI-MS is applied here, for the first time, to the analysis of secondary organic aerosol (SOA) formed from the reaction of ozone and a-pinene. The results are compared to those obtained using atmospheric pressure chemical ionization mass spectrometry (APCI-MS). The SOA was generated in the laboratory and merged with electrospray droplets. The recovered ions were directed towards the inlet of a triple quadrupole mass spectrometer. Through the use of a denuder to remove gas phase compounds, the EESI-MS technique was found to be effective for measuring the major ozonolysis products either in particles alone or in a combination of vapor phase and particulate products. Due to its relatively simple setup and the avoidance of sample collection and work-up, EESI-MS shows promise as an excellent tool for the characterization of atmospherically relevant particles.

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Development of extractive electrospray ionization ion trap mass spectrometry for in vivo breath analysis

Cited by 55 publications

References 65 publications

Fast detection of volatile organic compounds from Staphylococcal blood cultures by CDI-MS

Fast detection of volatile organic compounds from Staphylococcal blood cultures by CDI-MS

On the mechanism of extractive electrospray ionization (EESI) in the dual-spray configuration

Analysis of secondary organic aerosols in air using extractive electrospray ionization mass spectrometry (EESI-MS)

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