Fast detection of volatile organic compounds from Staphylococcal blood cultures by CDI-MS

Hang, Yaping; Chingin, Konstantin; Liang, Juchao; Wang, Xiaozhong; Hu, Longhua

doi:10.1039/c7ra01815k

Cited by 8 publications

(9 citation statements)

References 25 publications

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“…It is commonly found in human faeces as a product of E. coli activity in the human gut 58 , and its high abundance is likely responsible for the characteristic foul odour of the culture. The detection of 1-alcohols such as 1-butanol, 1-hexanol, 1-octanol, and 1-decanol was in agreement with existing literature 22 , 59 , 60 . Indole is the major VOC produced by E. coli 9 and has been documented as an intercellular signal molecule amongst diverse bacteria 61 .…”

Section: Discussionsupporting

confidence: 91%

Multi-strain volatile profiling of pathogenic and commensal cutaneous bacteria

Fitzgerald

Duffy

Holland

et al. 2020

Sci Rep

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The detection of volatile organic compounds (VOC) emitted by pathogenic bacteria has been proposed as a potential non-invasive approach for characterising various infectious diseases as well as wound infections. Studying microbial VOC profiles in vitro allows the mechanisms governing VOC production and the cellular origin of VOCs to be deduced. However, inter-study comparisons of microbial VOC data remains a challenge due to the variation in instrumental and growth parameters across studies. In this work, multiple strains of pathogenic and commensal cutaneous bacteria were analysed using headspace solid phase micro-extraction coupled with gas chromatography–mass spectrometry. A kinetic study was also carried out to assess the relationship between bacterial VOC profiles and the growth phase of cells. Comprehensive bacterial VOC profiles were successfully discriminated at the species-level, while strain-level variation was only observed in specific species and to a small degree. Temporal emission kinetics showed that the emission of particular compound groups were proportional to the respective growth phase for individual S. aureus and P. aeruginosa samples. Standardised experimental workflows are needed to improve comparability across studies and ultimately elevate the field of microbial VOC profiling. Our results build on and support previous literature and demonstrate that comprehensive discriminative results can be achieved using simple experimental and data analysis workflows.

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Section: Discussionsupporting

confidence: 91%

Multi-strain volatile profiling of pathogenic and commensal cutaneous bacteria

Fitzgerald

Duffy

Holland

et al. 2020

Sci Rep

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“…The headspace VOCs of cultures were continuously diverted into the ionization region with nitrogen gas flow (0.1 Mpa, 1 L/min). The device from the inlet of the culture to the inlet of the LTQ (ThermoFischer) capillary was previously introduced in detail 10 .…”

Section: Methodsmentioning

confidence: 99%

“…MALDI-TOF MS has been widely accepted and gradually applied in clinical laboratories and is based on the differences of bacterial protein spectra 8,9 . These three different methods exhibit their own shortcomings 10 . In our study, the evident potential advantages of homemade and new high-sensitivity ambient corona discharge ionization MS (CDI-MS) is ultimately rapid (several hours), accurate and with high-throughput without any pretreatment (ca.…”

Section: Introductionmentioning

confidence: 99%

“…10–20 s per sample). Therefore, the method is suitable for simple real-time analysis and the potential for direct bacteria screening 10,11 . Furthermore, this method could simultaneously detect both polar and non-polar analytes with high sensitivity, showing promising potential as an application for the rapid detection of various compounds present in complex matrices 12 .…”

Section: Introductionmentioning

confidence: 99%

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Profiles of volatile indole emitted by Escherichia coli based on CDI-MS

Zhong

Feng

Liang

et al. 2019

Sci Rep

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Escherichia coli is an important pathogen of nosocomial infection in clinical research, Thus, exploring new methods for the rapid detection of this pathogen is urgent. We reported the early release of molecular volatile indole vapour of E. coli cultures and blood cultures analyzed by direct atmospheric corona discharge ionization mass spectrometry (CDI-MS). The concentration of indole in E. coli cultures remarkably increases during the early log and lag phases of bacterial growth, thereby enabling early detection. Technical replicates were cultivated for 3 days for reference diagnosis using current conventional bacteraemia detection. A reference MS screen of common microbes from other genera confirmed that the peaks at m/z 116 signal corresponded to indole were specifically present in E. coli. Our results indicated that volatile indole based on CDI-MS without the need for any sample pretreatment is highly suitable for the reliable and cost-efficient differentiation of E. coli, especially for bacteraemia in humans.

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“…Staphylococcus aureus ( S. aureus ) is a ubiquitous pathogen that commonly colonizes in the nose, respiratory tract and also on the skin. Staphylococcus aureus usually survives in a commensal condition with other strains, and it often presents weak pathogenicity [ 1 , 2 ]. However, there were also identified pathogenic S. aureus strains, which produce virulence factors that cause a wide variety of diseases from skin infections (abscesses, folliculitis and scalded skin syndrome) to life-threatening disease (meningitis, osteomyelitis, endocarditis, sepsis and toxic shock syndrome) [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%