Development of extracellular vesicle-based medicinal products: A position paper of the group “Extracellular Vesicle translatiOn to clinicaL perspectiVEs – EVOLVE France”

Silva, Amanda; Morille, Marie; Piffoux, Max; Arumugam, Surendar; Mauduit, Philippe; Larghero, Jérôme; Bianchi, Arnaud; Aubertin, Kelly; Blanc‐Brude, Olivier; Noël, Danièle; Velot, Émilie; Ravel, Célia; Élie-Caille, Céline; Sebbagh, Anna; Boulanger, Chantal M.; Wilhelm, Claire; Rahmi, Gabriel; Raymond‐Letron, Isabelle; Cherukula, Kondareddy; Montier, Tristan; Martinaud, Christophe; Bach, Jean‐Marie; Favre-Bulle, Olivier; Spadavecchia, Jolanda; Jørgensen, Christian; Menasché, Philippe; Aussel, Claude; Chopineau, Joël; Mosser, Mathilde; Ullah, Matti; Sailliet, Nicolas; Luciani, Nathalie; Mathieu, N.; Rautou, Pierre‐Emmanuel; Brouard, Sophie; Boireau, Wilfrid; Jauliac, Sébastien; Dedier, M.; Trouvin, Jean‐Hugues; Gazeau, Florence; Trouillas, Marina; Peltzer, Juliette; Monsel, Antoine; Banzet, Sébastien

doi:10.1016/j.addr.2021.114001

Cited by 56 publications

(58 citation statements)

References 83 publications

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“…EVs origin, isolation method, monitoring). Initiatives emerge to propose precise recommendations about EVs handling (144) and are taken to grow. As far as we know, the choice of the isolation methods depends from the attempted perspectives since different protocols may alter different EV cargo compartment.…”

Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles in Transplantation

et al. 2022

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Extracellular vesicles (EVs) have been extensively studied in the last two decades. It is now well documented that they can actively participate in the activation or regulation of immune system functions through different mechanisms, the most studied of which include protein–protein interactions and miRNA transfers. The functional diversity of EV-secreting cells makes EVs potential targets for immunotherapies through immune cell-derived EV functions. They are also a potential source of biomarkers of graft rejection through donor cells or graft environment-derived EV content modification. This review focuses on preclinical studies that describe the role of EVs from different cell types in immune suppression and graft tolerance and on the search for biomarkers of rejection.

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Section: Discussionmentioning

confidence: 99%

Extracellular Vesicles in Transplantation

et al. 2022

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“…Due to their intrinsic capability to vehicle biological materials and information, EVs have high potential as drug delivery systems ( Armstrong and Stevens, 2018 ; Kooijmans et al, 2021 ). Indeed, there is an increasing interest to exploit EVs as therapeutics ( Witwer et al, 2019 ; Silva et al, 2021 ; Limongi et al, 2021b ) and in a large variety of biotechnological applications ( Paganini et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation of Extracellular Vesicles From Microalgae: A Renewable and Scalable Bioprocess

Paterna

Rao

Adamo

et al. 2022

Front. Bioeng. Biotechnol.

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Extracellular vesicles (EVs) play a crucial role as potent signal transducers among cells, with the potential to operate cross-species and cross-kingdom communication. Nanoalgosomes are a subtype of EVs recently identified and isolated from microalgae. Microalgae represent a natural bioresource with the capacity to produce several secondary metabolites with a broad range of biological activities and commercial applications. The present study highlights the upstream and downstream processes required for the scalable production of nanoalgosomes from cultures of the marine microalgae Tetraselmis chuii. Different technical parameters, protocols, and conditions were assessed to improve EVs isolation by tangential flow filtration (TFF), aiming to enhance sample purity and yield. The optimization of the overall bioprocess was enhanced by quality control checks operated through robust biophysical and biochemical characterizations. Further, we showed the possibility of recycling by TFF microalgae cells post-EVs isolation for multiple EV production cycles. The present results highlight the potential of nanoalgosome production as a scalable, cost-effective bioprocess suitable for diverse scientific and industrial exploitations.

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“…In this regard, EVs-based therapies can be better standardized than cell-based therapies. Moreover, these therapies can be commercialized under a softer regulation pathway than cell-based therapies, being considered “biological medicines” [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Extracellular vesicles from hair follicle-derived mesenchymal stromal cells: isolation, characterization and therapeutic potential for chronic wound healing

Heras¹,

Royo

Garcia-Vallicrosa

et al. 2022

Stem Cell Res Ther

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Background Mesenchymal stromal cells (MSCs) and their extracellular vesicles (MSC-EVs) have demonstrated to elicit immunomodulatory and pro-regenerative properties that are beneficial for the treatment of chronic wounds. Thanks to different mediators, MSC-EVs have shown to play an important role in the proliferation, migration and cell survival of different skin cell populations. However, there is still a big bid to achieve the most effective, suitable and available source of MSC-EVs. Methods We isolated, characterized and compared medium-large EVs (m-lEVs) and small EVs (sEVs) obtained from hair follicle-derived MSCs (HF-MSCs) against the gold standard in regenerative medicine, EVs isolated from adipose tissue-derived MSCs (AT-MSCs). Results We demonstrated that HF-EVs, as well as AT-EVs, expressed typical MSC-EVs markers (CD9, CD44, CD63, CD81 and CD105) among other different functional markers. We showed that both cell types were able to increase human dermal fibroblasts (HDFs) proliferation and migration. Moreover, both MSC-EVs were able to increase angiogenesis in human umbilical vein endothelial cells (HUVECs) and protect HDFs exposed to a hyperglycemic environment from oxidative stress and cytotoxicity. Conclusions Taken together, HF-EVs demonstrated to exhibit comparable potential to that of AT-EVs as promising candidates in the treatment of chronic wounds.

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Development of extracellular vesicle-based medicinal products: A position paper of the group “Extracellular Vesicle translatiOn to clinicaL perspectiVEs – EVOLVE France”

Cited by 56 publications

References 83 publications

Extracellular Vesicles in Transplantation

Extracellular Vesicles in Transplantation

Isolation of Extracellular Vesicles From Microalgae: A Renewable and Scalable Bioprocess

Extracellular vesicles from hair follicle-derived mesenchymal stromal cells: isolation, characterization and therapeutic potential for chronic wound healing

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