Development of enzyme immunoassay for the detection of triazine herbicides

Dunbar, Bohn; Riggle, Bruce D.; Niswender, Gordon D.

doi:10.1021/jf00092a021

Cited by 77 publications

(23 citation statements)

References 14 publications

(22 reference statements)

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“…As observed in other reported atrazine ELISAs [16][17][18], the herbicide propazine was better recognized than atrazine, with a crossreactivity value of 313% in the microplate format. The herbicide terbutylazine, frequently used in Germany, alone or in combination with other triazine herbicides, is also recognized.…”

Section: Selectivity Of the Test Kitsupporting

confidence: 75%

Evaluation of a field-test kit for triazine herbicides (SensioScreen® TR500) as a fast assay to detect pesticide contamination in water samples

Ballesteros

Barceló

Dankwardt³

et al. 2003

Analytica Chimica Acta

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A field-test kit (SensioScreen ® TR500) for the determination of triazine herbicides in water samples has been evaluated. The test is based on an ELISA method performed on a membrane that allows the visual estimation of the presence of triazine herbicides in less than ten minutes. The kit contains all necessary reagents and equipment, and operates on a very simple protocol. The detection level of this semiquantitative kit has been set at 0.5 µg L -1 (for the sum of all triazines), although concentration levels of 0.1 µg L -1 for atrazine can be detected. This is the first time that a commercial field-test kit to control water contamination by herbicides performs in compliance with the EU and USA legislations regarding the limit of detection reached. Evaluation and validation studies have been performed using spiked, certified (AquaCheck) and real environmental samples comparing the results with chromatographic methods. In spite of the semiquantitative-qualitative character of the kit, the results demonstrate that the test SensioScreen TR500 provides a very good estimation of the atrazine concentration in the sample. This test format could be adapted to the detection of other contaminants of environmental relevance.

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Section: Selectivity Of the Test Kitsupporting

confidence: 75%

Evaluation of a field-test kit for triazine herbicides (SensioScreen® TR500) as a fast assay to detect pesticide contamination in water samples

Ballesteros

Barceló

Dankwardt³

et al. 2003

Analytica Chimica Acta

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“…Therefore, the CRSO% is not significant in this field, though it is widely used. 19,21,22 The new definition of cross-reactivity gives more information, because besides the center point it also examines the slope of the curve.…”

Section: Discussionmentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay for the Determination of 2,4,6-Trinitrotoluene and Related Nitroaromatic Compounds

Keuchel

Well

1992

ANAL. SCI.

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A sensitive enzyme-linked Immunosorbent assay (ELISA) for the determination of the explosive 2,4,6-trinitrotoluene (TNT) and other nitroaromatic compounds was developed. As tracer, a trinitrophenyl-derivative conjugated to horseradish peroxidase was used. With this competitive assay, performed in polystyrene microtitre plates, TNT can be detected within the range of 0.02 µg/1 and 20 µg/ 1. Cross-reactivities at the center points (50% values) of several nitroaromatic compounds were determined. Additionally, the cross-reactivities at different concentrations of the analytes were stated to yield a more realistic estimation of the interferences. Finally, the applicability of the immunoassay to the determination of TNT in water samples was assessed.

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“…Atrazine aminocaproic acid 2 was synthesized according to published procedures [34]. Branched polyethylenimine (bPEI, average molecular weight ca.…”

Section: Reagentsmentioning

confidence: 99%

Self‐Assembling of Redox‐Active Atrazine Poly(ethylenimine) Conjugates – Interfacial Electrochemical and Spectroscopic Characterization

et al. 2006

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International audienceThin films of polymers tethered with both atrazine haptens and ferrocenyl (Fc) probes were formed on polycrystalline gold electrodes by taking advantage of the facile formation of self-assembled monolayers (SAMs) of thiolates. These films were characterized by polarization modulation Fourier transform infrared reflection – absorption spectrometry (PM-IRRAS), X-ray photoelectron spectroscopy (XPS) and cyclic voltammetry. The combination of these techniques gave a full insight into the structure and the binding mode of the polymers and provided useful quantitative information about both Fc entity and atrazine hapten surface density. This may open the way to a new type of immunosensor for atrazine monitoring

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Development of enzyme immunoassay for the detection of triazine herbicides

Cited by 77 publications

References 14 publications

Evaluation of a field-test kit for triazine herbicides (SensioScreen® TR500) as a fast assay to detect pesticide contamination in water samples

Evaluation of a field-test kit for triazine herbicides (SensioScreen® TR500) as a fast assay to detect pesticide contamination in water samples

Enzyme-Linked Immunosorbent Assay for the Determination of 2,4,6-Trinitrotoluene and Related Nitroaromatic Compounds

Self‐Assembling of Redox‐Active Atrazine Poly(ethylenimine) Conjugates – Interfacial Electrochemical and Spectroscopic Characterization

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