Development of enzyme-cartridge flow-injection analysis for industrial process monitoring

Purification by Immunoaffinity Chromatography, Characterization, and Structural Analysis of a Thermostable Pyranose Oxidase from the White Rot Fungus Phlebiopsis gigantea

“…At 50ЊC, the half-life of the Phlebiopsis gigantea PROD is 100 days, and even at 70ЊC, the Pleurotus ostreatus enzyme is stable for 2 h. However, there are significant differences among PRODs with respect to operational stability, a most important criterion for biotechnological use of an enzyme. While both products of D-glucose oxidation, 2-keto-D-glucose and the byproduct H 2 O 2 , caused rapid inactivation of the PROD from Polyporus obtusus (31), this was not the case for the Peniophora gigantea PROD, which has an economically useful half-life of 9 days under the operating conditions found in an enzyme reactor (12) and in an enzyme cartridge for process monitoring (16). Other differences among PRODs may be attributable to their primary structures and are reflected by the differences in their isoelectric points, activation energies, and immunologic specificities (13).…”

“…Because of their selective mode of action, PRODs were used as highly efficient biocatalysts in synthetic carbohydrate chemistry for the production of industrially significant compounds (9,10,12,17,20,27,38). In addition, analytical applications of PROD have been reported in clinical chemistry (35,39) as well as in industrial-process monitoring (16). Altogether, the biotechnical importance of PRODs is reflected by the existence of ample patent literature on these enzymes (27).…”

Ethanol biosensors based on alcohol oxidase

“…A wide range of substrates has been used, including chromogenic, fluorigenic and luminogenic substrates. One of the most popular substrate used is 2,2 -azino-di(3-ethylbenzthiazoline-6-sulfonate), ABTS (Majkic-Singh and Berkes, 1980;Jürgens et al, 1994;Rhee and Schügerl, 1997;Ukeda et al, 1999). The oxidised form of ABTS has a bluish green colour and is usually detected around 415 nm.…”

Pyranose oxidase biosensor based on carbon nanotube (CNT)-modified carbon paste electrodes

“…Since its first characterization by Janssen and Ruelius, POx has received attention as the key biocatalysts in various biotechnological applications; especially analytical applications in clinical chemistry, in monitoring industrial processes, in food preservation and in synthetic carbohydrate chemistry [25][26][27][28][29]. POx has also been used as a biological component in biosensor [18,19,24,27,[30][31][32][33][34][35]. Despite the large-scale industrial use of GOx, there are two main potential advantages in using POx instead of GOx.…”