Ethanol biosensors based on alcohol oxidase

Azevedo, Ana M.; Prazeres, D.M.F.; Cabral, Joaquim M. S.; Fonseca, Luís P.

doi:10.1016/j.bios.2004.09.030

Cited by 227 publications

(102 citation statements)

References 81 publications

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“…Although reports of SBP-based 163 biosensors are emerging, HRP biosensor research dominates and has continued to 164 develop through many forms, from the traditional voltammetric-and amperometric-165 based methods of detection, to nano-sized devices. Real time quantification of 166 hydrogen peroxide continues to be one of the main reasons for sensor development 167 [44], although other diverse applications include the detection of glucose [45], ethanol 168 [46] and tumour markers in vivo [47]. Enzyme-based biosensors require rapid and 169 uniform transfer of electrons generated at the enzyme active site to the transducer.…”

Section: Device That Brings Together An Immobilised Biological Sensinmentioning

confidence: 99%

Horseradish and soybean peroxidases: comparable tools for alternative niches?

Ryan

Carolan

Ó’Fágáin

2006

Trends in Biotechnology

139

102

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Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful 9 biotechnological tools. HRP is often termed the classical plant heme peroxidase, and 10 although it has been studied for decades our understanding has deepened since its 11 cloning and subsequent expression, which has enabled numerous mutational and 12 protein engineering studies. SBP, however, has been neglected until recently; despite 13 offering a real alternative to HRP that actually outperforms it in terms of stability. 14 SBP is now used in numerous biotechnological applications, including biosensors. 15Review of both is timely. This article summarises and discusses the main insights into 16 the structure and mechanism of HRP, with special emphasis on HRP mutagenesis, and 17 outlines its use in a variety of applications. It also reviews current knowledge and 18 applications to date of SBP, particularly biosensors. The final paragraphs speculate on 19 the future of plant heme-based peroxidases, with probable trends outlined and 20 explored. 21 22

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Section: Device That Brings Together An Immobilised Biological Sensinmentioning

confidence: 99%

Horseradish and soybean peroxidases: comparable tools for alternative niches?

Ryan

Carolan

Ó’Fágáin

2006

Trends in Biotechnology

139

102

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“…Â³í äîçâîëÿº îáèðàòè òà ï³äòðèìóâàòè ðîçì³ð, ôîðìó òà òîâùèíó ìàò-ðèö³ ³ çàáåçïå÷óº ÷³òêèé êîíòðîëü çà ïðîöåñîì îñàäaeåííÿ [11]. Êð³ì òîãî, îòðèìàí³ ³ç çàñòîñó-âàííÿì äàíîãî ìåòîäó íàï³âïðîíèêí³ ïîë³ìåðí³ ïë³âêè ìîaeóòü âèñòóïàòè ó ÿêîñò³ ñåëåêòèâíîãî áàð`ºðó äëÿ åëåêòðîõ³ì³÷íî àêòèâíèõ ³íòåðôå-ðóþ÷èõ ÷àñòîê [13].…”

Section: ²ììîá³ë³çàö³ÿ ëîä øëÿõîì åëåêòðîõ³ì³÷íî¿ ïîë³ìåðèçàö³¿ ó ïîëunclassified

Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

Paryzhak

Demkiv

Schuhmann³

et al. 2008

SEMST

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“…Alcohol oxidase is catalytically active for alcohols containing short alkyl chains, alcohol dehydrogenase is more selective for alcohols other than methanol as the corresponding reaction rate decreases rapidly with the size of the molecule [12]. As discussed in a recent review [13], the use of alcohol oxidase enzyme on the analysis of ethanol in complex samples allows a considerable enhancement in specificity. Examples of such a sensor developed for the analysis of beer have also been presented [14,15].…”

Section: Introductionmentioning

confidence: 99%

Simple and Rapid Determination of Ethanol Content in Beer Using an Amperometric Biosensor

2015

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An alcohol dehydrogenase-based biosensor was prepared and tested for its use to determine ethanol in beer. The biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide and immobilized with a biocatalytic layer containing the enzyme. Function of the enzyme biosensor was tested in model ethanol samples, in which it showed a linear range of 15-120 g•L −1 with a detection limit of 3.3 g•L −1 (established as 3σ) and response time of 19 s. In a potential window from -0.2 to +0.45 V, interferences of both ascorbic and uric acids were negligible. Several types of marketed beers of Czech provenance were selected and subjected to measurements under optimized conditions but without any pretreatment of real samples. When compared with the reference method (gas chromatography), the results were in quite good agreement for beers of the pale lager type but higher contents of ethanol were indicated in the samples of dark lager beers.

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Ethanol biosensors based on alcohol oxidase

Cited by 227 publications

References 81 publications

Horseradish and soybean peroxidases: comparable tools for alternative niches?

Horseradish and soybean peroxidases: comparable tools for alternative niches?

Intact Recombinant Cells of the Yeast Hansenula Polymorpha, Over-Producing Formaldehyde Dehydrogenase, as the Sensitive Bioelements for Amperometric Assay of Formaldehyde

Simple and Rapid Determination of Ethanol Content in Beer Using an Amperometric Biosensor

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