Development of electrochemical biosensor for virus dengue diagnostic using graphite electrode

Souza, E. Martins De; Nascimento, G.A.; Santana, N.A.; Bruneska, Danyelly; Filho, J. L. Lima

doi:10.1016/j.nbt.2009.06.932

Cited by 3 publications

(2 citation statements)

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“…The electrode that worked only with poly-l-lysine presented no current peaks, which showed that the polymer did not interfere with the result. At the same time, the electrode that worked with modified oligonucleotides demonstrated current peaks in the potential range characteristic of an oxi-reduction process between DNA guanines and methylene blue [74]. The polymer had no intervention effect on the result because only the working electrode, which was modified with oligonucleotides, showed current peaks, but no signal was obtained when using a poly-l-lysine-modified electrode.…”

Section: Genomic Detectionmentioning

confidence: 96%

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An introduction to dengue-disease diagnostics

Darwish

Alias

Khor

2015

TrAC Trends in Analytical Chemistry

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Section: Genomic Detectionmentioning

confidence: 96%

“…In another genosensor, Martins et al [74] presented pencillead graphite (PLG) as a low cost, renewable electrode. A DNA probe with a complementary sequence was electrostatically immobilized onto a PLG electrode that was fabricated with poly-l-lysine solution.…”

Section: Genomic Detectionmentioning

confidence: 99%

An introduction to dengue-disease diagnostics

Darwish

Alias

Khor

2015

TrAC Trends in Analytical Chemistry

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Electrochemical DNA biosensor for bovine papillomavirus detection using polymeric film on screen-printed electrode

Nascimento

Souza

Campos-Ferreira

et al. 2012

Biosensors and Bioelectronics

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Label-Free Electrochemical Detection of the Specific Oligonucleotide Sequence of Dengue Virus Type 1 on Pencil Graphite Electrodes

Souza

Nascimento

Santana

et al. 2011

Sensors

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A biosensor that relies on the adsorption immobilization of the 18-mer single-stranded nucleic acid related to dengue virus gene 1 on activated pencil graphite was developed. Hybridization between the probe and its complementary oligonucleotides (the target) was investigated by monitoring guanine oxidation by differential pulse voltammetry (DPV). The pencil graphite electrode was made of ordinary pencil lead (type 4B). The polished surface of the working electrode was activated by applying a potential of 1.8 V for 5 min. Afterward, the dengue oligonucleotides probe was immobilized on the activated electrode by applying 0.5 V to the electrode in 0.5 M acetate buffer (pH 5.0) for 5 min. The hybridization process was carried out by incubating at the annealing temperature of the oligonucleotides. A time of five minutes and concentration of 1 μM were found to be the optimal conditions for probe immobilization. The electrochemical detection of annealing between the DNA probe (TS-1P) immobilized on the modified electrode, and the target (TS-1T) was achieved. The target could be quantified in a range from 1 to 40 nM with good linearity and a detection limit of 0.92 nM. The specificity of the electrochemical biosensor was tested using non-complementary sequences of dengue virus 2 and 3.

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Development of electrochemical biosensor for virus dengue diagnostic using graphite electrode

Cited by 3 publications

References 0 publications

An introduction to dengue-disease diagnostics

An introduction to dengue-disease diagnostics

Electrochemical DNA biosensor for bovine papillomavirus detection using polymeric film on screen-printed electrode

Label-Free Electrochemical Detection of the Specific Oligonucleotide Sequence of Dengue Virus Type 1 on Pencil Graphite Electrodes

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