An introduction to dengue-disease diagnostics

Darwish, Nadiya Taha; Alias, Yatimah; Khor, Sook Mei

doi:10.1016/j.trac.2015.01.005

Cited by 34 publications

(28 citation statements)

References 91 publications

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“…Teles argue that there is thus a role that microfabricated devices can play to facilitate dengue diagnosis (Teles, 2011). Readers can refer to more extensive review by Darwish et al on conventional means of dengue diagnostic (Darwish et al, 2015).…”

Section: Other Infectious Diseases (Sars Dengue Tuberculosis)mentioning

confidence: 99%

Advances in microfluidics in combating infectious diseases

Tay

Pavesi

Yazdi

et al. 2016

Biotechnology Advances

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One of the important pursuits in science and engineering research today is to develop low-cost and user-friendly technologies to improve the health of people. Over the past decade, research efforts in microfluidics have been made to develop methods that can facilitate low-cost diagnosis of infectious diseases, especially in resource-poor settings. Here, we provide an overview of the recent advances in microfluidic devices for point-of-care (POC) diagnostics for infectious diseases and emphasis is placed on malaria, sepsis and AIDS/HIV. Other infectious diseases such as SARS, tuberculosis, and dengue are also briefly discussed. These infectious diseases are chosen as they contribute the most to disability-adjusted life-years (DALYs) lost according to the World Health Organization (WHO). The current state of research in this area is evaluated and projection toward future applications and accompanying challenges are also discussed.

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Section: Other Infectious Diseases (Sars Dengue Tuberculosis)mentioning

confidence: 99%

Advances in microfluidics in combating infectious diseases

Tay

Pavesi

Yazdi

et al. 2016

Biotechnology Advances

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“…Dengue is an infection caused by the RNA virus DENV, a member of the Flavivirus genus (Parkash and Shueb, 2015). DENV virus has four different serotypes (DENV1, DENV2, DENV3 and DENV4) which can cause, depending on the incidence (first or secondary infection) (Darwish et al, 2015;Sinawang et al, 2016), dengue fever (DF), dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS) (Darwish et al, 2015). The symptoms vary from those which have little physical presentation to a severe disease status as characterized by bleeding and circulatory failure.…”

Section: Introductionmentioning

confidence: 99%

“…The symptoms vary from those which have little physical presentation to a severe disease status as characterized by bleeding and circulatory failure. Dengue is transmitted from one person to another by the bites (Darwish et al, 2015) of a female contaminated Aedes mosquito (Teles, 2011), which propagates in urban areas in subtropical and tropical regions (Darwish et al, 2015). Frequent outbreaks are reported in Asia-Pacific region, both central and south America and southeast of the Gulf of Mexico (Darwish et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Dengue is transmitted from one person to another by the bites (Darwish et al, 2015) of a female contaminated Aedes mosquito (Teles, 2011), which propagates in urban areas in subtropical and tropical regions (Darwish et al, 2015). Frequent outbreaks are reported in Asia-Pacific region, both central and south America and southeast of the Gulf of Mexico (Darwish et al, 2015). In Brazil alone, over a million cases of dengue were registered from January to May of 2015 and, in 2016, an increase of about 50% was registered in the first two months of the year, over 2015 levels.…”

Section: Introductionmentioning

confidence: 99%

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A dual marker label free electrochemical assay for Flavivirus dengue diagnosis

Santos

Bueno

Davis

2018

Biosensors and Bioelectronics

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Dengue is a RNA viral illness of the genus Flavivirus which can cause, depending on the pervasiveness of the infection, hemorrhagic dengue fever or dengue shock syndrome. Herein we present an electrochemical label free approach enabling the rapid sensitive quantification of NS1 and IgG (supporting an ability to distinguish primary and secondary infections). Using a bifunctional SAM containing PEG moieties and a tethered redox thiol, both markers are detectable across clinically relevant levels by label free impedance derived redox capacitance. A subsequent frequency specific immittance function approach enables assaying (within seconds) with no impairment of analytical quality (linearity, sensitivity and variance).

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“…The former of these, virus isolation, although a reliable confirmation method, takes time as well as requiring specialized equipment and a high level of technical expertise [6]. A range of serological tests based on detection of virus-specific antibodies are commercially available and have been extensively evaluated [7]. The most common of these methods is enzyme-linked immunosorbent assay (ELISA), detection based on immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies to DENV [8].…”

Section: Introductionmentioning

confidence: 99%

Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor

Rahman

Yusof

Hashim

et al. 2016

Analytica Chimica Acta

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Dengue Virus (DENV) has become one of the most serious arthropod-borne viral diseases, causing death globally. The existing methods for DENV detection suffer from the late stage treatment due to antibodies-based detection which is feasible only after five days following the onset of the illness. Here, we demonstrated the highly effective molecular electronic based detection utilizing silicon nanowire (SiNW) integrated with standard complementary metal-oxide-semiconductor (CMOS) process as a sensing device for detecting deoxyribonucleic acid (DNA) related to DENV in an early stage diagnosis. To transform the fabricated devices as a functional sensing element, three-step procedure consist of SiNW surface modification, DNA immobilization and DNA hybridization were employed. The detection principle works by detecting the changes in current of SiNW which bridge the source and drain terminal to sense the immobilization of probe DNA and their hybridization with target DNA. The oxygen (O) plasma was proposed as an effective strategy for increasing the binding amounts of target DNA by modified the SiNW surface. It was found that the detection limit of the optimized O plasma treated-SiNW device could be reduced to 1.985 × 10 M with a linear detection range of the sequence-specific DNA from 1.0 × 10 M to 1.0 × 10 M. In addition, the developed biosensor device was able to discriminate between complementary, single mismatch and non-complementary DNA sequences. This highly sensitive assay was then applied to the detection of reverse transcription-polymerase chain reaction (RT-PCR) product of DENV-DNA, making it as a potential method for disease diagnosis through electrical biosensor.

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An introduction to dengue-disease diagnostics

Cited by 34 publications

References 91 publications

Advances in microfluidics in combating infectious diseases

Advances in microfluidics in combating infectious diseases

A dual marker label free electrochemical assay for Flavivirus dengue diagnosis

Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor

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