Development of effective cryopreservation protocols using aluminium cryo-plates for mulberry

Tanaka, Daisuke; Yamamoto, S.; Matsumoto, T.; Arizaga, Miriam Valle; Niino, Toshiki

doi:10.17660/actahortic.2019.1234.34

Cited by 6 publications

(1 citation statement)

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“…We therefore applied the alginate gel with 2 M of glycerol and 0.4 M of sucrose, lowered than the 2 M of glycerol and 0.8 M of sucrose of the subsequent preculture/loading step, for attaching shoot tips on foils. In developing the V cryo-plate protocol for cryopreservation of mint and mulberry, Yamamoto et al ( 2012) and Tanaka et al (2019) showed respectively that the regrowth level was not affected with the addition of sucrose (at 0.4 M) in alginate gel. The present study did not optimize the solutes of the alginate gel, as it may have quite limited in uence on the regrowth of cryopreserved shoot tips, as suggested by Yamamoto et al ( 2012) and Tanaka et al (2019).…”

Section: Discussionmentioning

confidence: 99%

Vitrification Cryo-Foil for Apple Cryopreservation and the Seesaw Effect Between Shoot Recovery and Virus Eradication

Wang

Jiang

et al. 2021

Preprint

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Improvements of existing cryopreservation protocols are necessary to facilitate long-term preservation of plant germplasm and the cryotherapy-effect of pathogen eradication. This study reported a vitrification (V) cryo-foil/plate methods for cryopreservation of shoot tips and cryotherapy effect in ‘Pink Lady’ apple. In V cryo-foil/plate protocols, shoot tips were first attached onto aluminum foils/plates using calcium alginate before other procedures. Shoot tips cryopreserved by V cryo-foil required 6.1 weeks to fully recover and 53% of shoot regrowth was obtained, comparable to the Dv cryopreservation. Similar regrowth levels were produced between applying V cryo-foil and Dv cryopreservation to another 4 Malus genotypes. Histological observations in shoot tips cryopreserved by Dv and V cryo-foil found only those with surviving apical dome and leaf primordia (LPs) could recover after cryopreservation. In apical meristem of shoot tips cryopreserved by Dv and V cryo-foil, higher surviving probability was detected from the V cryo-foil protocol, and the young LPs showed the highest level of surviving. Virus detection in cryo-derived plants showed apple stem grooving virus and apple chlorotic leaf spot virus were all preserved after cryopreservation, and higher eradication efficiency of apple stem pitting virus (70%) was produced by Dv than the 55% of V cryo-foil. These results supported applying V cryo-foil as an improvement to the widely applied Dv method in shoot tip cryopreservation, and also revealed a seesaw mode between shoot recovery and cryotherapy effect. Once the seesaw moves to increase the recovery after cryopreservation, the cryotherapy-effect on the other side would be decreased.

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Section: Discussionmentioning

confidence: 99%