2020
DOI: 10.1007/s11240-020-01770-0
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Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources

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Cited by 79 publications
(96 citation statements)
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“…This method can guarantee the biodiversity conservation of a high number of species in the long term and at a low cost. However, this technique is not feasible for those species with recalcitrant seeds (not tolerant to reduction of their water content and to low temperature), with asexual reproduction (e.g., hybrid species) or with high heterozygosity [ 6 ]. For these types of germplasm, in vitro conservation (slow growth) techniques and cryopreservation are successful options.…”
Section: Epigenetic Variation In Ex Situ Plant Conservation: the Rmentioning
confidence: 99%
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“…This method can guarantee the biodiversity conservation of a high number of species in the long term and at a low cost. However, this technique is not feasible for those species with recalcitrant seeds (not tolerant to reduction of their water content and to low temperature), with asexual reproduction (e.g., hybrid species) or with high heterozygosity [ 6 ]. For these types of germplasm, in vitro conservation (slow growth) techniques and cryopreservation are successful options.…”
Section: Epigenetic Variation In Ex Situ Plant Conservation: the Rmentioning
confidence: 99%
“…In order to avoid ice crystal formation and/or desiccation damage in cells, two main types of cryopreservation techniques have been developed [ 6 , 94 ]. Some are based on a controlled decrease of temperature and the use of cryoprotectants, forming extracellular ice crystals and causing the cells to dehydrate to the point where they would turn to a glass (vitrify).…”
Section: Cryopreservationmentioning
confidence: 99%
“…A more recent development in plant cryopreservation is the establishment of two cryo-plate vitrification methods: the vitrification cryo-plate (V Cryo-plate [106]) and dehydration cryo-plate (D Cryo-plate [81,107]). These methods follow the same principles as the droplet vitrification method; small volumes (droplets) cool down more rapidly to the temperature of liquid nitrogen compared to big volumes.…”
Section: Droplet Vitrificationmentioning
confidence: 99%
“…Some advantages of the V Cryo-plate and D Cryo-plate method are (1) simple procedure to store plates in liquid nitrogen, (2) processing large numbers at a time and (3) secured high cooling and heating speed [108]. Post-cryopreservation regeneration rates of these methods are not significantly different from the droplet vitrification method [81], therefore, the choice of method to use is personal preference.…”
Section: Droplet Vitrificationmentioning
confidence: 99%
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