Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources

Lambardi, Maurizio; Engelmann, Florent; Pathirana, Ranjith; Panis, Bart; Volk, Gayle M.; Wang, Qiao‐Chun

doi:10.1007/s11240-020-01770-0

Cited by 79 publications

(96 citation statements)

References 92 publications

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“…This method can guarantee the biodiversity conservation of a high number of species in the long term and at a low cost. However, this technique is not feasible for those species with recalcitrant seeds (not tolerant to reduction of their water content and to low temperature), with asexual reproduction (e.g., hybrid species) or with high heterozygosity [ 6 ]. For these types of germplasm, in vitro conservation (slow growth) techniques and cryopreservation are successful options.…”

Section: Epigenetic Variation In Ex Situ Plant Conservation: the Rmentioning

confidence: 99%

“…In order to avoid ice crystal formation and/or desiccation damage in cells, two main types of cryopreservation techniques have been developed [ 6 , 94 ]. Some are based on a controlled decrease of temperature and the use of cryoprotectants, forming extracellular ice crystals and causing the cells to dehydrate to the point where they would turn to a glass (vitrify).…”

Section: Cryopreservationmentioning

confidence: 99%

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Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers.

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Section: Epigenetic Variation In Ex Situ Plant Conservation: the Rmentioning

confidence: 99%

Section: Cryopreservationmentioning

confidence: 99%

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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“…A more recent development in plant cryopreservation is the establishment of two cryo-plate vitrification methods: the vitrification cryo-plate (V Cryo-plate [106]) and dehydration cryo-plate (D Cryo-plate [81,107]). These methods follow the same principles as the droplet vitrification method; small volumes (droplets) cool down more rapidly to the temperature of liquid nitrogen compared to big volumes.…”

Section: Droplet Vitrificationmentioning

confidence: 99%

“…Some advantages of the V Cryo-plate and D Cryo-plate method are (1) simple procedure to store plates in liquid nitrogen, (2) processing large numbers at a time and (3) secured high cooling and heating speed [108]. Post-cryopreservation regeneration rates of these methods are not significantly different from the droplet vitrification method [81], therefore, the choice of method to use is personal preference.…”

Section: Droplet Vitrificationmentioning

confidence: 99%

“…Routine cryopreservation of crop collections began only a few decades ago. Currently, about 18 genebanks have cryopreserved crop collections [13,67,81,98,109]. It is estimated that about 100,000 unique accessions of vegetatively propagated and recalcitrant seed crops potentially need long-term conservation through cryopreservation while currently only about 10,000 accessions are cryopreserved [13].…”

Section: Droplet Vitrificationmentioning

confidence: 99%

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Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Panis

Nagel

houwe

2020

Plants

Self Cite

104

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The conservation of crop genetic resources, including their wild relatives, is of utmost importance for the future of mankind. Most crops produce orthodox seeds and can, therefore, be stored in seed genebanks. However, this is not an option for crops and species that produce recalcitrant (non-storable) seeds such as cacao, coffee and avocado, for crops that do not produce seeds at all; therefore, they are inevitably vegetatively propagated such as bananas, or crops that are predominantly clonally propagated as their seeds are not true to type, such as potato, cassava and many fruit trees. Field, in vitro and cryopreserved collections provide an alternative in such cases. In this paper, an overview is given on how to manage and setup a field, in vitro and cryopreserved collections, as well as advantages and associated problems taking into account the practical, financial and safety issues in the long-term. In addition, the need for identification of unique accessions and elimination of duplicates is discussed. The different conservation methods are illustrated with practical examples and experiences from national and international genebanks. Finally, the importance of establishing safe and long-term conservation methods and associated backup possibilities is highlighted in the frame of the global COVID-19 pandemic.

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Building capacity in the conservation of exceptional plant species

Philpott

Pence

Coffey³

2022

Appl Plant Sci

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Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources

Cited by 79 publications

References 92 publications

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Challenges and Prospects for the Conservation of Crop Genetic Resources in Field Genebanks, in In Vitro Collections and/or in Liquid Nitrogen

Building capacity in the conservation of exceptional plant species

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