Development of Diagnostic Reagents To Differentiate between<i>Mycobacterium bovis</i>BCG Vaccination and<i>M. bovis</i>Infection in Cattle

Vordermeier, H. M.; Cockle, P; Whelan, Adam O.; Rhodes, Shelley; Palmer, N.; Bakker, Douwe; Hewinson, R. Glyn

doi:10.1128/cdli.6.5.675-682.1999

Cited by 135 publications

(50 citation statements)

References 37 publications

(43 reference statements)

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“…Mammalian and avian tuberculin preparations (purified protein derivative [PPD]‐M and PPD‐A), and recombinant proteins ESAT‐6, MPB70 and MPB83 were produced as described by Vordermeier et al . 17 at the Veterinary Laboratories Agency (Weybridge, UK). Recombinant MPB64 was kindly provided by D. Bakker (Animal Health Science, Boxtel, the Netherlands).…”

Section: Methodsmentioning

confidence: 99%

Bovine tuberculosis: immune responses in the peripheral blood and at the site of active disease

et al. 2000

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Summary This report describes a comparison of immune responses in the peripheral blood and at the site of active disease in cattle 20 weeks after experimental infection with Mycobacterium bovis. Lymphocyte proliferation, and the production of interferon‐γ (IFN‐γ) and interleukin (IL)‐2 were measured in response to tuberculin and a number of mycobacterial antigens, including ESAT‐6, MPB64, MPB70, MPB83, hsp 16.1, hsp 65, hsp 70 and the 38 000 MW lipoprotein antigen. The level of transforming growth factor‐β (TGF‐β) was measured following stimulation of cells with tuberculin. Our results suggest little difference in the responses of peripheral blood and lymph node cells to most of the antigens used. However, tuberculin purified protein derivative (PPD) and ESAT‐6 elicited stronger responses in the peripheral blood compared with lymph node cells. Investigation of the responding T‐cell subpopulations in the peripheral blood showed that both CD4+ and, to a lesser extent, γδ T‐cell receptor‐positive (TCR+) T cells contributed to these responses. This is the first report to compare peripheral and local immune responses in bovine tuberculosis. Unlike cases of human tuberculosis where immune activity at the site of disease and anergy in the peripheral blood have been reported, our results suggest that for bovine tuberculosis immune responses occurring in the peripheral blood reflect those at the site of disease.

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Section: Methodsmentioning

confidence: 99%

Bovine tuberculosis: immune responses in the peripheral blood and at the site of active disease

et al. 2000

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“…The mainstay of bovine tuberculosis control is the application of tuberculin skin testing and the removal of test-positive animals as part of so-called test and slaughter control policies. However, as is the case for BCG vaccination of humans, BCG vaccination of animals compromises the use of tuberculin-based assays such as skin testing or blood-based IFN-γ release assays due to the antigens shared between BCG and M. tuberculosis or M. bovis (12,(90)(91)(92). Thus, the development of so-called DIVA (differentiating infected from vaccinated animals) tests will be required for countries which intend to use vaccination of cattle alongside conventional test and slaughter control strategies.…”

Section: Antigen Mining and Diva Testsmentioning

confidence: 99%

Experimental Infection Models of Tuberculosis in Domestic Livestock

2016

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In this article we present experimental Mycobacterium bovis infection models in domestic livestock species and how these models were applied to vaccine development, biomarker discovery, and the definition of specific antigens for the differential diagnosis of infected and vaccinated animals. In particular, we highlight synergies between human and bovine tuberculosis (TB) research approaches and data and propose that the application of bovine TB models could make a valuable contribution to human TB vaccine research and that close alignment of both research programs in a one health philosophy will lead to mutual and substantial benefits.

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“…Interferon (IFN)‐γ responses to MPB70 have been explored for the diagnosis of tuberculosis together with several other antigens, but in general ESAT‐6 has been shown to induce stronger responses than MPB70 [48, 55–57]. On the other hand, MPB83 appear to induce stronger T‐cell responses in M. bovis infected cattle than MPB70 [58].…”

Section: Cellular Immune Responses To Mpb70mentioning

confidence: 99%

MPB70 and MPB83 – Major Antigens of Mycobacterium bovis

Wiker

2009

Scand J Immunol

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Early works on MPB70 and MPB83 (Mycobacterium bovis) or MPT70 and MPT83 (Mycobacterium tuberculosis)The first observations on MPB70 were likely made already in 1960 by Lind [1,2]. MPB70 was originally identified as a protein highly expressed by M. bovis Bacille CalmetteGuérin (BCG) substrain Tokyo by Nagai [3]. MPB70 has a relative mobility of 0.70 by native polyacrylamide gel electrophoresis at pH 9.4, and the term 'MPB' refers to a protein from M. bovis, hence its name while 'MPT' refers to proteins from M. tuberculosis. The protein was purified from BCG culture filtrate using conventional purification methods and was found to induce delayed type hypersensitivity in guinea pigs immunized with some BCG strains, but not by certain other BCG strains [4,5]. Antibody responses followed a similar pattern [5]. Two-dimensional electrophoresis showed the presence of MPB70 in the strains that induced delayed type hypersensitivity [4]. This was soon confirmed using rabbit antibodies raised against MPB70. These antibodies reacted with MPB70 and formed a distinct precipitation line in crossed immunoelectrophoresis [6]. By radioimmunoassay inhibition tests, it was possible to quantify MPB70 [6], and MPB70 was clearly demonstrated, but in very low amounts in the strains that were negative in the previous investigations [6]. The observations formed the basis for dividing BCG strains into two main groups, those that express high levels of MPB70 and those that express minor amounts of MPB70. Those that express high levels of MPB70 are BCG Tokyo, Moreau, Russia, Sweden, Birkhaug (Bergen), Romania (Cantacuzinho strain), and those that express low levels are BCG Pasteur, Danish 1331, Glaxo, Tice and Beijing. This subdivison of BCG strains has been correlated to how BCG was distributed from the Pasteur institute during the 1920s. The comparatively low levels of MPB70 in sonicated extracts of whole washed bacterial cells strongly indicated that MPB70 was actively secreted Abstract MPB70 and MPB83 are among the most studied mycobacterial antigens. They are highly homologous proteins within Mycobacterium tuberculosis complex members, and the orthologs in different members of the complex are virtually identical. They are major antigens highly expressed by Mycobacterium bovis and considerably less abundantly expressed by M. tuberculosis. There are two genes encoding these proteins within an operon of six genes. MPB70 and MPB83 are encoded as precursor proteins with typical signal peptides for export through the general secretory pathway. MPB70 is a soluble secreted protein cleaved by signal peptidase I, while MPB83 is a glycosylated lipoprotein processed by signal peptidase II and located at the surface, possibly with the lipid tail coupled to the N-terminal cystein embedded in the mycobacterial outer membrane. The expression of these genes is controlled by the transcriptional regulator SigK, and a point mutation in SigK explains why some Bacille Calmette-Guérin (BCG) strains express only minute amounts of MPB70 and MPB83. BCG strains t...

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Development of Diagnostic Reagents To Differentiate betweenMycobacterium bovisBCG Vaccination andM. bovisInfection in Cattle

Cited by 135 publications

References 37 publications

Bovine tuberculosis: immune responses in the peripheral blood and at the site of active disease

Bovine tuberculosis: immune responses in the peripheral blood and at the site of active disease

Experimental Infection Models of Tuberculosis in Domestic Livestock

MPB70 and MPB83 – Major Antigens of Mycobacterium bovis

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