Development of cryopreservation procedures for semen of Pacific bluefin tuna Thunnus orientalis

Gwo, H.-H.; Weng, Ting-Sheng; Fan, Lee-Shing; Lee, Yan‐Horn

doi:10.1016/j.aquaculture.2005.03.034

Cited by 21 publications

(8 citation statements)

References 26 publications

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“…2). Esto está reportado también por otros autores, los que muestran que a pesar de la mayor toxicidad de crioprotectores a mayores concentraciones, éstas tienen mejor efecto protector contra el congelamiento (Gwo 1993, Gwo et al 2005, Liu et al 2006, Tian et al 2008.…”

Section: Discussionunclassified

Criopreservación de espermatozoides de anchoveta peruana (Engraulis ringens)

Catcoparco

Dupré

Espinoza

2010

Rev. biol. mar. oceanogr.

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The present work shows a practical and of cheap methodology for Engraulis ringens sperm cryopreservation, with the purpose to optimize its reproduction techniques in captivity. The investigation was divided in three stages. The first one consisted to evaluate toxicity of four cryoprotectants; dimethylsulfoxide (ME 2 SO), ethanol (ET), propylene glycol (PG), glycerol (GL) to three molars concentrations (0.5 M; 1.0 M and 1.5 M) on spermatic motility. Motility percentages of sperms incubated in ME 2 SO were significantly bigger than that observed in others three cryoprotectants. In the second stage, the optimal freezing rates were determinate, using ME 2 SO only. In this case, the biggest motility percentages were obtained with 1.5 M ME 2 SO with freezing rates of-20 to-40 ºC min-1 (61.3 ± 7.6% and 53.8 ± 4.9% respectively). Finally, the effect of addition of not permeable cryoprotectant in cryoprotective solution (egg hen yolk, VHG) was evaluated to optimize the post-thawing spermatic motility; nevertheless, the results did not change significantly respect to control without VHG.

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Section: Discussionunclassified

Criopreservación de espermatozoides de anchoveta peruana (Engraulis ringens)

Catcoparco

Dupré

Espinoza

2010

Rev. biol. mar. oceanogr.

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“…Successful cryopreservation and later use of sperm has been reported in both aquatic and marine species (Bolla et al, 1987;Suquet et al, 2000;Billard et al, 2004;DeGraff and Berlinsky, 2004;Kang et al, 2004;Tiersch et al, 2004a,b;Riley et al, 2004;Gwo et al, 2005). Only Riley et al (2004) examined the long term, 2 years, effects of cryopreservation on post thaw viability of sperm.…”

Section: Cryopreservationmentioning

confidence: 99%

Aquaculture in bio-regenerative life support systems (BLSS): Considerations

Gonzales

2009

Advances in Space Research

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“…Substances such as 1‐2 propylene glycol (PG) and methanol (MetOH) are commonly used as cryoprotectants to freeze marine organisms' gametes. These substances showed good results in the male gametes freezing of fish (Gwo, Weng, Fan & Lee ; Marco‐Jiménez, Garzón, Peñaranda, Pérez, Viudes‐de‐Castro, Vicente, Jover & Asturiano ; Tian, Chen, Ji, Zhai, Sun, Chen & Su ), oysters (Paniagua‐Chavez & Tiersch ; Dong, Huang, Eudeline & Tiersch ; Dong, Huang, Eudeline, Allen & Tiersch ; Kawamoto, Narita, Isowa, Aoki, Hayashi, Komaru & Ohta ; Adams, Smith, Taylor, Tervit & McGowan ) and mussels (Di Matteo, Langellotti, Masullo & Sansone ), but at this moment the effects of these substances on the physiological parameters of Nodipecten nodosus spermatozoa are still unknown.…”

Section: Introductionmentioning

confidence: 99%

Sperm motility and fertilization performance ofNodipecten nodosus(L., 1758) exposed at two different cryoprotectants

et al. 2014

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Cryopreservation is a valuable tool for aquaculture as it provides continuous seed production, regardless of the spawning season of the brood stock. The selection of a suitable cryoprotectant with low toxicity and high water solubility is important to avoid membrane injuries and intracellular ice crystallization. This study has been aimed at the assessment of the toxic effects of two usually applied cryoprotectants, 1-2 propylene glycol (PG) and methanol (MetOH), on spermatozoa of the of lion-paw scallop Nodipecten nodosus, by evaluating the sperm motility and the development of D larvae after fertilization procedure. Sperm was exposed at room temperature (22°C) for 10, 20 and 30 min to different concentration ranges of two cryoprotectants. Regarding the sperm motility, PG5%, PG7%, MetOH4% and MetOH6% did not show differences compared to control (semen incubated in seawater) (P < 0.05). The development of D larvae was not affected by the exposition to PG5%, MetOH 4% and MetOH 6%. These results indicate the potential use of both cryoprotectants for cryopreservation procedures.

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Development of cryopreservation procedures for semen of Pacific bluefin tuna Thunnus orientalis

Cited by 21 publications

References 26 publications

Criopreservación de espermatozoides de anchoveta peruana (Engraulis ringens)

Criopreservación de espermatozoides de anchoveta peruana (Engraulis ringens)

Aquaculture in bio-regenerative life support systems (BLSS): Considerations

Sperm motility and fertilization performance ofNodipecten nodosus(L., 1758) exposed at two different cryoprotectants

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