Criopreservación de espermatozoides de anchoveta peruana (Engraulis ringens)

Abstract

The present work shows a practical and of cheap methodology for Engraulis ringens sperm cryopreservation, with the purpose to optimize its reproduction techniques in captivity. The investigation was divided in three stages. The first one consisted to evaluate toxicity of four cryoprotectants; dimethylsulfoxide (ME 2 SO), ethanol (ET), propylene glycol (PG), glycerol (GL) to three molars concentrations (0.5 M; 1.0 M and 1.5 M) on spermatic motility. Motility percentages of sperms incubated in ME 2 SO were significantly bigger than that observed in others three cryoprotectants. In the second stage, the optimal freezing rates were determinate, using ME 2 SO only. In this case, the biggest motility percentages were obtained with 1.5 M ME 2 SO with freezing rates of-20 to-40 ºC min-1 (61.3 ± 7.6% and 53.8 ± 4.9% respectively). Finally, the effect of addition of not permeable cryoprotectant in cryoprotective solution (egg hen yolk, VHG) was evaluated to optimize the post-thawing spermatic motility; nevertheless, the results did not change significantly respect to control without VHG.

Criopreservacion de espermatozoides del lenguado Paralichthys adspersus

“…Estos resultados concuerdan con aquellos obtenidos en diversos peces planos como lenguado de invierno Pseudopleuronectes americanus (Rideout et al, 2003), rodaballo Scophthalmus maximus ; lenguado de Argentina P. orbignyanus (Lanes et al, 2008) y róbalo manchado Verasper variegatus (Tian et al, 2008). Similares resultados fueron obtenidos por nuestro grupo de trabajo al criopreservar espermatozoides de anchoveta Engraulis ringens (Catcoparco et al, 2010). Todos estos estudios mostraron que el DMSO permite una mayor protección, respecto a otros crioprotectores, para los espermatozoides descongelados sometidos a congelación-descongelación.…”