Development of Cryo-banking System of Lilium Species

Development, progress and future prospects in cryobiotechnology of Lilium spp.

“…1Major steps of Lilium cryopreservation for shoot tips ( A ), pollen ( B ) and seeds ( C ). Shoot tip cryopreservation by droplet-vitrification (1 in A [35]; 2 in A [34, 43]), vitrification (3 in A [30, 31]), encapsulation–vitrification (4 in A [30]), and encapsulation–dehydration (5 in A [30]). Pollen cryopreservation by dehydration (B [53]).…”

“…callosum and 53–88 for the four Oriental hybrids[34] Lilium spp, Asiatic hybrid and Oriental hybrid20 Lilium species, 1 Asiatic hybrid and 1 Oriental hybridDroplet-vitrification52 for L. amabile , 67 for L . auratum, 43 for L. bulbiferum, 67 for L. callosum, 62 for L. candidum, 42 for L. concolor, 85 for L. davidii, 41 for L. distichum, 48 for L. formolongi , 86 for L. hansonii , 38 for L. henryi ,41 for L. lancifolium, 40 for L. leichtlinii , 69 for L. leucanthum , 43 for L. longiflorum , 41 for L. pyrenaicum, 83 for L. regale , 43 for L. tsingtauense , 41 L. washingtonianum, 43 for Asiatic hybrid, 73 for Oriental hybrid[43] L. davidii var. unicolor , L. × formolongi, L. longiflorum × Oriental ‘Triumphator’, Oriental hybrids and Asiatic hybrids3 species, 2 Oriental hybrids and 2 Asiatic hybridsDroplet-vitrification80 for L. davidii var.…”

“…Droplet-vitrification combines the advantages of droplet freezing with vitrification, thus allowing samples to obtain rapid cooling and warming rates, increasing the survival of cryopreserved samples [41, 42]. Droplet-vitrification is considered to be a robust means for the cryopreservation of Lilium shoot tips [32, 34–36, 38, 43].…”

“…Yi et al [34, 43] described a droplet-vitrification method for shoot tip cryopreservation of four Oriental hybrids and L. callosum . In their study, 2-week old in vitro stock cultures were cold-hardened for 7 days at 4 °C in a 16-h photoperiod of 35 µmol m −2 s −1 light intensity.…”

“…PVS3 has been found to be less toxic to the cells than PVS2 and appeared to better protect plant tissues against osmotic stress, thus resulting in better survival of shoot tips after cryopreservation [47]. Droplet-vitrification has been applied to cryopreservation of 160 Lilium accessions at the National Academy of Agricultural Sciences in South Korea, including 20 species, one Asiatic hybrid and one Oriental hybrid [43]. The highest and lowest levels of shoot regrowth in cryopreserved shoot tips were 86% for L. hansonii and 38% for L. henryi , averaging at 56% for the total Lilium germplasm tested [43].…”