Development of Cellulose Hydrogel Microspheres for Lipase Immobilization

Jo, Soyeon; Park, Saerom; Oh, Yong-Taek; Hong, Ji‐Yeon; Kim, Hyung Joo; Kim, Kwang Jin; Oh, Kyeong Keun; Lee, Sang Hyun

doi:10.1007/s12257-018-0335-0

Cited by 41 publications

(21 citation statements)

References 24 publications

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“…Therefore, it is of great importance to immobilize enzymes on solid carriers to improve their stabilities and achieve their facile recoveries during industrial applications ( Duan et al, 2019 ). To date, significant efforts have been devoted to immobilize enzymes onto various carriers and composite materials, such as hydrogels ( Jo et al, 2019 ), mesoporous silica ( Popat et al, 2011 ; Li et al, 2019 ), magnetic nanoparticles ( Desai and Pawar, 2020 ), polymers ( Kuiper et al, 2008 ), and proteins ( Kim et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Pickering Emulsion-Based Microreactors for Size-Selective Interfacial Enzymatic Catalysis

Lei

Liang

Luo

2020

Front. Bioeng. Biotechnol.

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In this study, we have developed a mild and effective method to prepare a metal-organic framework (MOF)-based microcapsule by the self-assembly of pre-synthesized zeolite imidazolate framework-8 (ZIF-8) nanoparticles at the oil-water interface combined with deposition of a dense ZIF-8 coating outside the capsule. By introducing the enzyme Candida antarctica lipase B (CalB) directly into the stabilizer ZIF-8 or the water phase of Pickering emulsion during the preparation process, we achieved that the enzyme was immobilized within the shell (CalB@ZIF-8@cap) or in the cavity (ZIF-8@cap-CalB) of the microcapsules, respectively. The resulting CalB-loaded microcapsules were robust and had a core-shell structure proved by scanning electron microscopy. Meanwhile, Fourier transform infrared spectroscopy was conducted to confirm the encapsulation of enzymes in the microcapsules and their position in the microcapsules was confirmed by fluorescence microscopy. Furthermore, through the comparison of transesterification reactions between a pair of small substrates and a pair of larger ones, the two types of CalB-loaded microcapsules showed great catalytic activity, stability and size selectivity, and the catalytic activity of CalB@ZIF-8@cap was slightly higher than that of ZIF-8@cap-CalB. Importantly, due to the large size of the microcapsules, the catalyst could be separated from the reaction system by sedimentation, thereby reducing the energy consumption for separation. These kinds of multifunctional MOF-enzyme composites may open up new opportunities for the biocatalysis and microreactor.

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Section: Introductionmentioning

confidence: 99%

Pickering Emulsion-Based Microreactors for Size-Selective Interfacial Enzymatic Catalysis

Lei

Liang

Luo

2020

Front. Bioeng. Biotechnol.

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“…Hydrogels are polymeric materials with three-dimensional network structures formed by hydrophilic polymers, which are swollen with a large volume of water [ 1 , 2 ]. Recently, because of their inherent biocompatibility, biodegradability, and non-toxicity, naturally derived biopolymer-based hydrogels have attracted tremendous attention in the biotechnological field for fabrication of protein supports, drug delivery carriers, tissue engineering scaffolds, biosensors, wound healing materials, etc., as well as for environmental applications such as fabrication of adsorbents for toxic compounds and supports for cell immobilization [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cellulose hydrogel microspheres are conventionally prepared through a sol-gel transition method using a solvent-in-oil emulsion. Previous studies have shown that the size and uniformity of the prepared cellulose hydrogel microspheres were affected by the ratio of solvent to oil, surfactant concentration, cellulose concentration, and stirring speed [ 2 , 22 , 23 ]. Peng et al prepared cellulose/chitosan/Fe 3 O 4 hydrogel microspheres for Cu(II) adsorption using an emulsion of 1-butyl-3-methylimidazolium chloride and vacuum oil [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we prepared cellulose hydrogel microspheres using 1-ethyl-3-methylimidazolium acetate ([Emim][Ac]) as enzyme supports [ 2 ]. The lipase immobilized on cellulose hydrogel microspheres showed a much higher loading efficiency, immobilization yield, and specificity constant than lipase immobilized on microcrystalline cellulose or millimeter-sized hydrogel beads, because of higher surface area and favorable interactions of cellulose microspheres.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe2O3 Hydrogel Microspheres as Enzyme Supports

Park

Jung

et al. 2020

Polymers

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Cellulose hydrogels are considered useful biocompatible and biodegradable materials. However, as few cellulose-dissolving solvents can be used to prepare cellulose hydrogel microspheres, the use of unmodified cellulose-based hydrogel microspheres for enzyme immobilization remains limited. Here, we prepared cellulose/Fe2O3 hydrogel microspheres as enzyme supports through sol-gel transition using a solvent-in-oil emulsion. Cellulose-dissolving solvents including 1-ethyl-3-methylimidazolium ([Emim][Ac]), an aqueous mixture of NaOH and thiourea, tetrabutylammonium hydroxide, and tetrabutylphosphonium hydroxide were used to prepare regular shaped cellulose/Fe2O3 microspheres. The solvent affected microsphere characteristics like crystallinity, hydrophobicity, surface morphology, size distribution, and swelling properties. The immobilization efficiency of the microspheres for lipase was also significantly influenced by the type of cellulose solvent used. In particular, the lipase immobilized on cellulose/Fe2O3 microspheres prepared using [Emim][Ac] showed the highest protein loading, and its specific activity was 3.1-fold higher than that of free lipase. The immobilized lipase could be simply recovered by a magnet and continuously reused.

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“…Microparticles are spherical particles with a diameter in the range of 1 µm to 1000 µm formed from synthetic or naturally occurring polymers [1,2]. Extracellular polymeric substances such as gellan gum [3,4], xanthan gum [5], alginate [6], hyaluronic acid [7], chitosan [8], and cellulose [9] or their mixtures have attracted the scientist's attention. Xanthan gum is a microbial polysaccharide produced by the bacterium Xanthomonas campestris through the fermentation process [10,11].…”

Section: Introductionmentioning

confidence: 99%

Modification of Collagen/Gelatin/Hydroxyethyl Cellulose-Based Materials by Addition of Herbal Extract-Loaded Microspheres Made from Gellan Gum and Xanthan Gum

et al. 2020

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Because consumers are nowadays focused on their health and appearance, natural ingredients and their novel delivery systems are one of the most developing fields of pharmacy, medicine, and cosmetics. The main goal of this study was to design, prepare, and characterize composite materials obtained by incorporation of microspheres into the porous polymer materials consisting of collagen, gelatin, and hydroxyethyl cellulose. Microspheres, based on gellan gum and xanthan gum with encapsulated Calendula officinalis flower extract, were produced by two methods: extrusion and emulsification. The release profile of the extract from both types of microspheres was compared. Then, obtained microparticles were incorporated into polymeric materials with a porous structure. This modification had an influence on porosity, density, swelling properties, mechanical properties, and stability of materials. Besides, in vitro tests were performed using mouse fibroblasts. Cell viability was assessed with the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The obtained materials, especially with microspheres prepared by emulsion method, can be potentially helpful when designing cosmetic forms because they were made from safely for skin ingredients used in this industry and the herbal extract was successfully encapsulated into microparticles.

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Development of Cellulose Hydrogel Microspheres for Lipase Immobilization

Cited by 41 publications

References 24 publications

Pickering Emulsion-Based Microreactors for Size-Selective Interfacial Enzymatic Catalysis

Pickering Emulsion-Based Microreactors for Size-Selective Interfacial Enzymatic Catalysis

Effect of Cellulose Solvents on the Characteristics of Cellulose/Fe2O3 Hydrogel Microspheres as Enzyme Supports

Modification of Collagen/Gelatin/Hydroxyethyl Cellulose-Based Materials by Addition of Herbal Extract-Loaded Microspheres Made from Gellan Gum and Xanthan Gum

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