Development of carbon dot based microplate and microfluidic chip immunoassay for rapid and sensitive detection of HIV-1 p24 antigen

Chunduri, L. A. Avinash; Haleyurgirisetty, Mohan; Patnaik, Sandeep; Bulagonda, Eswarappa Pradeep; Kurdekar, Aditya; Liu, Jikun; Hewlett, Indira; Venkataramaniah, K.

doi:10.1007/s10404-016-1825-z

Cited by 18 publications

(12 citation statements)

References 33 publications

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“…We used a SpectraMax M5 plate reader to perform the measurements in the FP mode. The FP values of unconjugated and streptavidin-conjugated nanoclusters from the FP equation were obtained by measuring the P and S polarization values based on the formula ( 20 )

where ∥ is the parallel polarized emission, ⊥ is the perpendicularly polarized emission, and G is the correction factor that is evaluated for the sensitivity of the instrument.…”

Section: Resultsmentioning

confidence: 99%

“…This was followed by final washing five times with PBS with Tween 20 buffer to reduce the background noise and prevent nonspecific interactions. Last, the sandwiched immunocomplex was excited with the excitation signal, and the emitted fluorescent signals from the sandwich immunocomplexes were read directly from the microplate in the end point format with the SpectraMax microplate reader (excitation at 345 nm and emission at 615 nm) ( 20 ). All experiments were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

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Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

Kurdekar

Chunduri²,

Manohar

et al. 2018

Sci. Adv.

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where ∥ is the parallel polarized emission, ⊥ is the perpendicularly polarized emission, and G is the correction factor that is evaluated for the sensitivity of the instrument.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

Kurdekar

Chunduri²,

Manohar

et al. 2018

Sci. Adv.

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“… S. No. Method P24 Concentration detected in pg/mL Reference 1 Conventional Elisa 10–15 16 2 Inductively coupled plasma mass spectrometry based gold nanoparticle immunoassay 7.5 35 3 Europium nanoparticle based immunoassay 0.5 15 4 Capacitive Immunosensor 0.12 36 5 Microchip based Europium nanoparticle immunoassay 5 37 6 Single-molecule immunosorbent assay 0.1 38 7 ZnO nanoparticle based immunoassay 25 39 8 Fluorescent Silver nanoparticles immunoassay 10 40 9 P24 antigen detection assay with booster step 0.5 17 10 Immunofluorescent cytometric bead assay 0.4 41 11 Carbon Dots based immunoassay 20 42 12 Silica Nanoparticle based immunoassay 0.02 Current Study …”

Section: Discussionmentioning

confidence: 99%

Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection

Chunduri

Kurdekar

Haleyurgirisetty

et al. 2017

Sci Rep

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We have engineered streptavidin labelled Europium doped fluorescent silica nanoparticles which significantly increased sensitivity without compromising the specificity of the immunoassay. As a proof of concept, a time resolved fluorescence based sandwich immunoassay was developed to detect HIV-1 p24 antigen in clinical specimens. The detection range of the silica nanoparticle based immunoassay (SNIA) was found to be between 0.02 to 500 pg/mL in a linear dose dependent manner. SNIA offers 1000 fold enhancement over conventional colorimetric ELISA. Testing of plasma samples that were HIV negative showed no false positive results in the detection of HIV-1 p24 antigen. This highly sensitive p24 assay can help improve blood safety by reducing the antibody negative window period in blood donors in resource limited settings where nucleic acid testing is not practical or feasible. This technology can also be easily transferred to a lab-on-a-chip platform for use in resource limited settings and can also be easily adopted for the detection of other antigens.

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“…The platform provided rapid on-chip detection in �5 min with a small sample volume of �15 μL and life-time reusability of >50 � . 3D) (Chunduri et al, 2016). 3C) (Coarsey et al, 2019).…”

Section: On-chip Immunoassaymentioning

confidence: 99%

Recent advances in lab-on-a-chip technologies for viral diagnosis

Zhu

Fohlerová

Pekárek

et al. 2020

Biosensors and Bioelectronics

190

146

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A B S T R A C TThe global risk of viral disease outbreaks emphasizes the need for rapid, accurate, and sensitive detection techniques to speed up diagnostics allowing early intervention. An emerging field of microfluidics also known as the lab-on-a-chip (LOC) or micro total analysis system includes a wide range of diagnostic devices. This review briefly covers both conventional and microfluidics-based techniques for rapid viral detection. We first describe conventional detection methods such as cell culturing, immunofluorescence or enzyme-linked immunosorbent assay (ELISA), or reverse transcription polymerase chain reaction (RT-PCR). These methods often have limited speed, sensitivity, or specificity and are performed with typically bulky equipment. Here, we discuss some of the LOC technologies that can overcome these demerits, highlighting the latest advances in LOC devices for viral disease diagnosis. We also discuss the fabrication of LOC systems to produce devices for performing either individual steps or virus detection in samples with the sample to answer method. The complete system consists of sample preparation, and ELISA and RT-PCR for viral-antibody and nucleic acid detection, respectively. Finally, we formulate our opinions on these areas for the future development of LOC systems for viral diagnostics.

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Development of carbon dot based microplate and microfluidic chip immunoassay for rapid and sensitive detection of HIV-1 p24 antigen

Cited by 18 publications

References 33 publications

Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

Femtogram Level Sensitivity achieved by Surface Engineered Silica Nanoparticles in the Early Detection of HIV Infection

Recent advances in lab-on-a-chip technologies for viral diagnosis

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