Development of capillary networks from rat microvascular fragments in vitro: The role of myofibroblastic cells

Satô, Noboru; Sawasaki, Yoshio; Senoo, Akira; Fuse, Yusuke; Hirano, Yoshiaki; Goto, Teppei

doi:10.1016/0026-2862(87)90017-3

Cited by 45 publications

(28 citation statements)

References 14 publications

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“…Both in vitro and in vivo, the tubular networks formed by monocultures of ECs such as HUVECs are not stable. Inclusion of mesenchymal-derived cells that can serve as pericytes such as the 10T1/2 cell line, embryonic and dermal fibroblasts, or hMSCs generates networks that are more robust and can anastomose to form functional vessels in vivo (5,7,9,11,28). Furthermore, it is suggested that 10T1/2 cells differentiate into SMCs or pericytes through heterotypic interactions with ECs (10,11,13).…”

Section: Discussionmentioning

confidence: 99%

“…Coculturing with an additional cell type to serve as mural cells and inclusion of ECM are necessary to maintain and mature the networks (5-11). The ability to precisely control in vitro experimental conditions directly led to identifying the mechanisms of many fundamental aspects of this process over the past several decades (5)(6)(7)(10)(11)(12)(13)(14). Several groups have recently shown that microvascular structures that are preformed in vitro by using endothelial and smooth muscle progenitor cells can anastomose with the host vasculature when implanted in a nude mouse model (15)(16)(17)(18)(19)(20).…”

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confidence: 99%

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Engineered vascularized bone grafts

Tsigkou

Pomerantseva²,

Spencer

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

206

185

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Clinical protocols utilize bone marrow to seed synthetic and decellularized allogeneic bone grafts for enhancement of scaffold remodeling and fusion. Marrow-derived cytokines induce host neovascularization at the graft surface, but hypoxic conditions cause cell death at the core. Addition of cellular components that generate an extensive primitive plexus-like vascular network that would perfuse the entire scaffold upon anastomosis could potentially yield significantly higher-quality grafts. We used a mouse model to develop a two-stage protocol for generating vascularized bone grafts using mesenchymal stem cells (hMSCs) from human bone marrow and umbilical cord-derived endothelial cells. The endothelial cells formed tube-like structures and subsequently networks throughout the bone scaffold 4–7 days after implantation. hMSCs were essential for stable vasculature both in vitro and in vivo; however, contrary to expectations, vasculature derived from hMSCs briefly cultured in medium designed to maintain a proliferative, nondifferentiated state was more extensive and stable than that with hMSCs with a TGF-β-induced smooth muscle cell phenotype. Anastomosis occurred by day 11, with most hMSCs associating closely with the network. Although initially immature and highly permeable, at 4 weeks the network was mature. Initiation of scaffold mineralization had also occurred by this period. Some human-derived vessels were still present at 5 months, but the majority of the graft vasculature had been functionally remodeled with host cells. In conclusion, clinically relevant progenitor sources for pericytes and endothelial cells can serve to generate highly functional microvascular networks for tissue engineered bone grafts.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Engineered vascularized bone grafts

Tsigkou

Pomerantseva²,

Spencer

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

206

185

View full text Add to dashboard Cite

show abstract

“…In the present experiments, we investigated whether acute ethanol exposure would cause a transient or stable inhibition of endothelial cell cord formation in vitro. The cord formation assay represents an in vitro system by which one can assess endothelial differentiation into capillary tubes, which is reminiscent of what occurs in vivo (24,30,43). Initially, we treated endothelial cells with increasing doses of ethanol, ranging from 50 to 300 mg/dl to identify the minimal inhibitory concentration for cord formation in the presence of VEGF.…”

Section: Acute Ethanol Exposure Induces a Stable Inhibition Of Endothmentioning

confidence: 99%

Acute ethanol exposure disrupts VEGF receptor cell signaling in endothelial cells

Radek

Kovacs

Gallo

et al. 2008

American Journal of Physiology-Heart and Circulatory Physiology

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“…Investigations into vascular endothelial cell growth factors, such as acid/basic fibroblast growth factor and platelet-derived endothelial cell growth factor (Sato et al 1987), also seem warranted.…”

Section: Results and Disussionmentioning

confidence: 99%

“…The capillary cord density per defined area was determined by superimposing a transparent rectangular grid (6.9 mm spacing) and then counting the total number of intersections between capillary cords and grids, as previously described (Yamashita et al 1993b). In this angiogenesis model, capillary growth could be conveniently and quantitatively estimated without the use of fetal bovine serum (including various vascular endothelial cell growth factors), namely, under serum-free conditions, unlike previous methods (Sato et al 1987). The protein content of each muscle was measured by the method of Lowry et al (1951).…”

Section: Methodsmentioning

confidence: 99%

Chronic Exposure to Simulated Altitude Does Not Increase Angiogenic Activity in Skeletal Muscle of Rats.

Yamashita

Nakanishi

Tajima

et al. 1994

Tohoku J. Exp. Med.

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In order to examine whether the hypoxia of high altitude increases angiogenic activity in skeletal muscle, six male Wistar rats were subjected to a simulated altitude of about 5,500 m (ambient pressure 380 mmHg) for 3 weeks, and the whole soleus, gastrocnemius, and extensor digitorum longus muscles were collected. As a result, any muscle extracts from high altitude rats did not significantly enhance the capillary growth in an in vitro angiogenesis model compared with those from sea-level rats. This appeared to confirm previous morphological studies that hypobaric-hypoxic environment did not cause the formation of new capillaries in skeletal muscles, simulated altitude; angiogenesis; skeletal muscle One obvious way to improve tissue diffusion under conditions of oxygen deprivation such as high altitude is to reduce the intra-capillary distance (Ward et al. 1989). For example, Cassin et al. (1971) found that the number of capillaries per unit surface area in skeletal muscle of rats exposed to 5,100 m for 5 weeks increased significantly. This result has been confirmed by other studies (Ward et al. 1989). However, Banchero (1975 has pointed out that the evidence of an increased capillarity in hypoxic conditions and its role in facilitating 02 delivery is not conclusive. Actually, Banchero (1975) has shown that although capillary density increases in skeletal muscle of dogs with a 3-week exposure to a simulated altitude of 4,880 m, this is not caused by the formation of new capillaries, but by

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Development of capillary networks from rat microvascular fragments in vitro: The role of myofibroblastic cells

Cited by 45 publications

References 14 publications

Engineered vascularized bone grafts

Engineered vascularized bone grafts

Acute ethanol exposure disrupts VEGF receptor cell signaling in endothelial cells

Chronic Exposure to Simulated Altitude Does Not Increase Angiogenic Activity in Skeletal Muscle of Rats.

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