Acute ethanol exposure disrupts VEGF receptor cell signaling in endothelial cells

Radek, Katherine A.; Kovacs, Elizabeth J.; Gallo, Richard L.; DiPietro, Luisa A.

doi:10.1152/ajpheart.00699.2007

Cited by 58 publications

(64 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, higher dose at 10% EtOH inhibit angiogenesis. Inhibition of angiogenesis has also been reported in studies carried out on chick embryo and on wound healing (Radek, Matthies et al 2005;Radek, Kovacs et al 2008). This suggests that EtOH inhibit the angiogenesis at higher doses.…”

Section: Discussionmentioning

confidence: 74%

Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo

Laghari¹

2015

AVS

View full text Add to dashboard Cite

Abstract:The developing Chick has been widely used as a model organism for research studies in developmental biology.Various aspects of ethanol (EtOH) toxicity during embryonic development have been documented in experimental studies. The studies on chick embryo address the effects of EtOH on craniofacial abnormalities and angiogenesis. The purpose of this study was to assess the effects of a single dose of ethanol on survival rate and angiogenesis of chick embryos during early stages of development. Chick eggs were incubated at 37ºC in the humidified incubator, after 72 hours of incubation these eggs were removed from incubator, and treated with a single doses of 1%, 5%, 10%, 13% and 15% EtOH. The effects on survival rate and angiogenesis were recorded on ethanol treated chick embryos. The survival rate was slightly reduced with 1% and 5% EtOH, however with increasing dose of 10% the survival rate was reduced to 64% and at 15% EtOH complete death was observed. Our results also indicate that treatment of EtOH with 1% and 5% did not have any obvious effects on vessels formation in comparison with the BSS treated embryos. However, treatment of chick embryo with 10%, 13% and 15% EtOH severely inhibited the blood vessels formation.

show abstract

Section: Discussionmentioning

confidence: 74%

Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo

Laghari¹

2015

AVS

View full text Add to dashboard Cite

show abstract

“…Relevant murine models demonstrate that acute alcohol consumption decreases the expression of VEGF receptors and reduces nuclear expression of HIF-1α in endothelial cells, thereby affecting angiogenesis and the proliferative phase of wound healing [69,70]. There are also studies showing that inappropriate alcohol consumption has an adverse effect on the physiologic reepithelialisation and collagen production [69][70][71][72].…”

Section: Inappropriate Alcohol Consumption Is Linked To Non-physiologmentioning

confidence: 99%

Impaired wound healing: facts and hypotheses for multi-professional considerations in predictive, preventive and personalised medicine

Avishai¹,

2017

View full text Add to dashboard Cite

Whereas the physiologic wound healing (WH) successfully proceeds through the clearly defined sequence of the individual phases of wound healing, chronic non-healing wounds/ulcers fail to complete the individual stages and the entire healing process. There are many risk factors both modifiable (such as stress, smoking, inappropriate alcohol consumption, malnutrition, obesity, diabetes, cardio-vascular disease, etc.) and non-modifiable (such as genetic diseases and ageing) strongly contributing to the impaired WH. Current statistics demonstrate that both categories are increasingly presented in the populations, which causes dramatic socio-economic burden to the healthcare sector and society at large. Consequently, innovative concepts by predictive, preventive and personalised medicine are crucial to be implemented in the area. Individual risk factors, causality, functional interrelationships, molecular signature, predictive diagnosis, and primary and secondary prevention are thoroughly analysed followed by the expert recommendations in this paper.

show abstract

“…AAA leads to insufficient immune system responses to infections; such deficiency was observed both in organ-specific [67][68][69] and systemic infections [70][71][72] . Acute alcohol intoxication impairs the ability of the host to counteract hemorrhagic shock [73] , augments corticosteroid release [74] and delays wound healing [75][76][77][78] , thus contributing to higher morbidity and mortality [79] and prolonged recovery from trauma [80] . The pathogenesis of AAA effects on human health is not fully understood.…”

Section: Aaa: Biomedical Impactmentioning

confidence: 99%

“…For example, if the desired alcohol concentration in the cell culture is 25 mmol/L, a Petri dish with 2 × the alcohol amount (50 mmol/L) was placed on the bottom of the chamber to ensure the saturation of the gas in the chamber); such conditions maintain the initial alcohol concentration ± 15% over a 24 h period [139] . However, depending on the scientific question of the study, the declining alcohol levels in vitro may be desired to mimic the alcohol elimination in vivo; in these situations the in vitro experiments are disadvantaged by the absence/limitation of alcohol metabolism [76,134] . The in vitro AAA model offers the possibility of primary in vitro exposure of alcohol-naive cells to alcohol alone or its combinations with diverse pharmacological or naturally-derived substances [24, but also the investigation of the effects of in vivo exposure to alcohol followed by ex vivo exposure to other stimulants [110,113,115] or vice versa.…”

Section: In Vitro Aaa Modelmentioning

confidence: 99%

In vitroandin vivomodels of acute alcohol exposure

Dolganiuc

Szabó

2009

WJG

View full text Add to dashboard Cite

Alcohol abuse is a global problem due to the financial burden on society and the healthcare system. While the harmful health effects of chronic alcohol abuse are well established, more recent data suggest that acute alcohol consumption also affects human wellbeing. Thus, there is a need for research models in order to fully understand the effect of acute alcohol abuse on different body systems and organs. The present manuscript summarizes the interdisciplinary advantages and disadvantages of currently available human and non-human models of acute alcohol abuse, and identifies their suitability for biomedical research.

show abstract

Acute ethanol exposure disrupts VEGF receptor cell signaling in endothelial cells

Abstract: Radek KA, Kovacs EJ, Gallo RL, DiPietro LA. Acute ethanol exposure disrupts VEGF receptor cell signaling in endothelial cells.

Cited by 58 publications

References 54 publications

Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo

Effects of a Single Dose of Ethanol on Survival Rate and Angiogenesis of Chick Embryo

Impaired wound healing: facts and hypotheses for multi-professional considerations in predictive, preventive and personalised medicine

In vitroandin vivomodels of acute alcohol exposure

Contact Info

Product

Resources

About