Development of canine oocytes matured and fertilised in vitro

Otoi, Takeshige; Murakami, M.; Fujii, Motoyuki; Tanaka, M.; Ooka, Andreas; Une, Satoshi; Suzuki, Takahiro

doi:10.1136/vr.146.2.52

Cited by 61 publications

(70 citation statements)

References 10 publications

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“…The difference in the relationship between the incubation period and maturation rates of oocytes is unclear, but our results indicate that the maximum rate of meiotic maturation of the oocytes collected from ovaries at the follicular phase occurs after 72 h of in vitro culture. Songsasen et al [12] obtained canine embryos at the cleavage stage after IVM/ IVF, in which the oocytes were matured for 48 h. England et al [10] demonstrated a single pregnancy after transferring 1-and 2-cell zygotes/embryos from IVF of canine oocytes matured between 24 and 72 h. Only one study reported the production of a single blastocyst from oocytes matured for 72 h followed by IVF [11]. However, an optimum maturation period for in vitro development of canine oocytes after IVF remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…In the IVM of canine oocytes, some studies have demonstrated that full meiotic maturation occurs after 24-48 h of in vitro culture [4,5], while other studies indicate that the oocytes cultured for 72-96 h achieved the highest maturation rates [6][7][8]. Yamada et al [9] suggested that oocytes from superovulated bitches achieve the highest maturation rates at 72 h of maturation culture, while oocytes from non-superovulated bitches show no tendency for increased maturation rates on culturing for up to 144 h. On the contrary, only four reported studies have shown the development of in vitro-matured and fertilized oocytes to the early embryo stage [9][10][11][12]. In the four studies, the oocytes were collected from bitches at various oestrous stages, matured for various incubation times (24-72 h) and fertilized with spermatozoa.…”

Section: Introductionmentioning

confidence: 99%

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Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization

et al. 2004

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-The objective of this study was to determine an optimum maturation period of canine oocytes for the development in vitro after in vitro fertilization (IVF). Canine oocytes larger than 110 micrometers in diameter, which were collected from ovaries at the follicular phase of the reproductive cycle, were cultured for each time (48, 72 and 96 h) in TCM 199 medium supplemented with 10% canine serum, fertilized, and then cultured in vitro for 8 days. Significantly more oocytes reached metaphase II (MII) in the 72-h culture group than in the 48-h culture group (25.6% vs. 41.0%). The percentages of oocytes that reached MII or beyond after maturation culture did not differ significantly between the 72-and 96-h culture groups, but the percentage of parthenogenetically activated oocytes in the 96-h culture group was significantly higher than that in the 72-h culture group. The percentages of cleaved embryos after IVF were significantly higher in the 48-and 72-h culture groups than in the 96-h culture group. In the 48-h culture group, 3.9% of fertilized oocytes developed to the 16-cell stage or beyond, but none of the cleaved embryos in the 72-and 96-h culture groups developed to the same stage. These results indicate that full nuclear maturation of oocytes collected from ovaries at the follicular phase occurs after 72 h of in vitro culture. However, an optimum maturation period (48 h) for the in vitro development of canine oocytes after IVF may be different from the period necessary to reach the maximal oocyte maturation rate, when based on the developmental stage of the cleaved embryos.developmental competence / incubation duration / meiotic maturation / oestrous stage

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization

et al. 2004

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“…Furthermore, the formation of canine 8-cell embryos was obtained after 96 h of IVF and culture, representing 2.4% of the total oocytes fertilized in vitro. Otoi et al (2000) reported 13% in vitro fertilized canine oocytes, but only 0.5% oocytes used in the experiment reached the phase of initial blastocyst. According to these authors, canine oocytes could be developed to the blastocyst stage after IVM, IVF and culture, although the competence of the oocyte developing is significantly reduced after these procedures.…”

Section: Recovery Of Oocytes Included In Antral Folliclesmentioning

confidence: 99%

The potential for gamete recovery from non-domestic canids and felids

Silva

Morato

Silva

2004

Animal Reproduction Science

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“…It was also suggested that COC maturation is correlated with the ability of oocytes and embryos to reach developmental competence or potential Kempisty et al, 2011b). Since in canine species only 20% of ovarian oocytes reach the MII stage, several well recognized media supplements are available which may increase the low number of oocytes that reach full maturity (Otoi et al, 2000;England et al, 2001;Mrazek and Fulka, 2003).…”

Section: Mammalian Coc Maturationmentioning

confidence: 99%

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

Kempisty¹,

Bukowska²,

Piotrowska³

et al. 2011

Vet. Med. - Czech

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ABSTRACT:Maturation of oocytes is the most important stage, which determines the subsequent successful fertilization, zygote formation, attainment of blastocyst stage, normal embryo growth and development, as well as appropriate implantation. Since in some species of mammals the efficiency of IVM maturation is still very low, many studies have focused on new combinations of media supplements. In some species of mammals, including pigs, mice, bovines, goats and dogs, EGF, BSA, and progesterone are successfully used as an enhancer of the IVC system. Application of the Lab-on-Chip system in reproductive biology opens new possibilities for the development of techniques available for the assessment of the developmental competency or potency manifested by mammalian oocytes and embryos. In most cases, the Lab-on-Chip system was used in mice. However, an increasing number of examples are available in which the system has been applied to evaluate porcine and bovine gametes and embryos. In this article, the role of EGF, BSA and progesterone is described in relation to maturation efficiency of mammalian oocytes. Moreover, the possibilities of using microfluidics (Lab-on-Chip) for the detection of oocyte developmental competency are also shown.

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Development of canine oocytes matured and fertilised in vitro

Cited by 61 publications

References 10 publications

Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization

Influence of maturation culture period on the development of canine oocytes after in vitro maturation and fertilization

The potential for gamete recovery from non-domestic canids and felids

Selected molecular and microfluidic aspects of mammalian oocyte maturation-perspectives: a review

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