Development of breed identification markers derived from AFLP in beef cattle

Sasazaki, Shinji; Itoh, Kenji; Arimitsu, S.; Imada, T.; Takasuga, Akiko; Nagaishi, H.; Takano, Shizuko; Mannen, Hideyuki; Tsuji, Soichi

doi:10.1016/j.meatsci.2003.10.016

Cited by 46 publications

(35 citation statements)

References 16 publications

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“…Food labeling regulations require that the species of meat in meat products to be accurately declared to the consumer [10]. It is vital for preventing possible commercial frauds and guaranteeing the quality and the safety of meat [11]. It is very important to assess that species of high commercial value are not sold, partially or entirely substituted with other species of lower commercial value [7].…”

Section: Species Identification Of Raw and Processed Fish And Meatmentioning

confidence: 99%

“…In livestock animals, DNA markers have been used for pedigree registration, individual identification, parentage test and removal of carrier individuals with genetic diseases [11]. Molecular DNA markers have become essential tools for the identification of marine species [30,31].…”

Section: Species Identification Of Raw and Processed Fish And Meatmentioning

confidence: 99%

“…In order to compete, Japan-based beef producers worked to create a first filial hybrid (F1) which is a cross of Japanese Black bulls with Holstein cows. FI beef was misbranded as Japanese Black beef, since the two breeds cannot be easily distinguished by appearance [11]. In China, there are many laws to protect the Chinese water deer (Hydropotes inermis Swinhoe), the Chinese muntjac (Muntiacus reevesi reevesi Ogilby), the black muntjac (Muntiacus crinifrons Sclater) and the muping tufted deer (Elaphodus cephalophus michianus) Swinhoe deer from illegal trade and poachers [42].…”

Section: Species Identification Of Raw and Processed Fish And Meatmentioning

confidence: 99%

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Forensics: Food Authentication Using MtDNA

Murugaiah¹

2015

JNHFS

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There is a clear trend in the food international market towards labelling products with information about their composition and quality. Due to the increase in international trade in seafood and seafood products, authentication has become a necessity. There is a need for suitable methods of identification to ensure compliance with the labelling regulations and thereby to prevent the substitution of fish species.

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Section: Species Identification Of Raw and Processed Fish And Meatmentioning

confidence: 99%

Section: Species Identification Of Raw and Processed Fish And Meatmentioning

confidence: 99%

Section: Species Identification Of Raw and Processed Fish And Meatmentioning

confidence: 99%

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Forensics: Food Authentication Using MtDNA

Murugaiah¹

2015

JNHFS

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“…In order to select the best marker combinations for discriminating Hanwoo and Hereford breeds, the identification and misjudgement probabilities were calculated based on the procedures described in Sasazaki et al (2004). This calculation was considered the marker allele frequencies between two populations and gave the possibilities of identification and misjudgement using these marker combinations.…”

Section: Calculation Of Identification Probabilitymentioning

confidence: 99%

Discrimination of Korean Cattle (Hanwoo) with Imported Beef from USA Based on the SNP Markers

Shim¹,

Seo²,

Seo³

et al. 2010

Korean Journal for Food Science of Animal Resources

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Due to the large amount of beef imported from the USA to Korea, Korean consumers have become increasingly interested in the country of origin since it can affect market prices. Previously, Bos indicus and Bos taurus-specific markers were developed for the purpose of cattle breed identification, specifically discrimination of Australian beef. In this study, six SNP markers derived from Illumina 50K bovine SNP chip data were used for the discrimination between Korean cattle (Hanwoo) and imported beef from USA. PCR-RFLP genotyping methods were also developed, which indicates that these markers can be applied relatively easily compared to other markers. Taking into account a discrimination rate of 55% based on MC1R marker between Hanwoo and imported beef from USA, two additional markers, SNPs 23803 and 34776, were ideal and resulted in probability of identification of 0.942 and probability of misjudgment of 0.03. Therefore, the markers developed in this study can greatly contribute to the correct discrimination between beef from USA and Hanwoo beef.

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“…RAPD, AFLP and RFLP have been applied to identification of animal components [13][14][15][16][17]. The problem and limitation with these wholegenome techniques is that there are always issues with reproducibility and when complex mixtures of two or more species are to be detected, interpretation of results may be difficult due to overlapping restriction patterns that may be generated [18].…”

Section: Introductionmentioning

confidence: 99%

A multiplex assay to identify 18 European mammal species from mixtures using the mitochondrial cytochrome b gene

Tobe

Linacre

2008

Electrophoresis

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A novel species-specific multiplex to identify 18 common European mammalian species (badger, cat, cow, dog, donkey, fox, goat, guinea pig, harvest mouse, hedgehog, horse, house mouse, human, pig, rabbit, rat, red deer and sheep), many of which are often associated with forensic investigations, has been developed. The assay is based on the mitochondrial cytochrome b gene, which is commonly used in species identification and phylogeny studies. Areas of homology and variation were identified and were used to create universal and species-specific primers. The species-specific primers were designed such that they will only react with the species for which they were designed. Two primer sets were designed for each species making the test self-confirmatory. All primer sets produced the expected results. The multiplex was balanced at template concentration of 40 000 copies (approximately 1.36 pg). Validation was accomplished by analysing the same sample ten times to determine run variation and several samples for each species to determine between-sample variation. Twenty-eight additional mammalian species were reacted with the multiplex. The multiplex provides, for the first time, a definitive method for identification of species in a forensic context.

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Development of breed identification markers derived from AFLP in beef cattle

Cited by 46 publications

References 16 publications

Forensics: Food Authentication Using MtDNA

Forensics: Food Authentication Using MtDNA

Discrimination of Korean Cattle (Hanwoo) with Imported Beef from USA Based on the SNP Markers

A multiplex assay to identify 18 European mammal species from mixtures using the mitochondrial cytochrome b gene

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