Development of biotin molecule targeted cancer cell drug delivery of doxorubicin loaded κ-carrageenan grafted graphene oxide nanocarrier

Vinothini, Kandasamy; Rajendran, Naresh Kumar; Munusamy, Murugan A.; Alarfaj, Abdulla A.; Rajan, Mariappan

doi:10.1016/j.msec.2019.03.011

Cited by 77 publications

(32 citation statements)

References 39 publications

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“…This is also a good evidence that PEGylation occurred regioselectively at both GO surfaces.The functionalization of GO sheets with PEG-Fol chains was also established by means of AFM and SEM microscopies (Figure 5).Figure 5ashows a typical dimensional distribution analysis of GO-PEG-Fol, where the presence of GO islands with a thickness of about 3.5 nm and a diameter of about 40 ± 4 nm can be observed. The increase of the nano-flakes thickness of about 2.5 nm can be explained with a homogeneous functionalization of the GO surface with Fol-PEG-CC chains of 2.5 kDa, corroborating UV and FTIR data [5,42]. It might be noticed that the small size and the narrow size distribution observed are suitable for biomedical applications, especially as carrier for anticancer drugs, since nanocarriers with dimensions between 20-50 nm allow enhanced in vivo bioavailability and tumor accumulation, thereby improving the effectiveness of anticancer treatments.…”

supporting

confidence: 78%

“…To the best of our knowledge, prior works have reported IC 50 from 3.4 to 18.25 g mL -1 , confirming that GO-PEG-Fol/Doxo nanoflakes have high pharmacological potency. [5,10,50] The same consideration can be made for MDA-MB-231 cells. [50][51][52] It is noteworthy that, comparable to the effect caused by free Doxo, GO-PEG-Fol/Doxo showed a selective cytotoxicity towards cancer cells, especially for MDA-MB-231.…”

Section: Physicochemical Stability Of Go-peg-fol/doxo In Aqueous Mediamentioning

confidence: 99%

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Folic acid-functionalized graphene oxide nanosheets via plasma etching as a platform to combine NIR anticancer phototherapy and targeted drug delivery

Mauro

Scialabba

Agnello

et al. 2020

Materials Science and Engineering: C

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PEGylated graphene oxide (GO) has shown potential as NIR converting agent to produce local heat useful in breast cancer therapy, since its suitable photothermal conversion, high stability in physiological fluids, biocompatibility and huge specific surface. GO is an appealing nanomaterial for potential clinical applications combining drug delivery and photothermal therapy in a single nanodevice capable of specifically targeting breast cancer cells. However, native GO sheets have large dimensions (0.5-5 m) such that tumor accumulation after a systemic administration is usually precluded. Herein, we report a step-by-step synthesis of folic acid-functionalized PEGylated GO, henceforth named GO-PEG-Fol, with small size and narrow size distribution (~30±5 nm), and the ability of efficiently converting NIR light into heat. GO-PEG-Fol consists of a nano-GO sheet, obtained by fragmentation of GO by means of non-equilibrium plasma etching, fully functionalized with folic acid-terminated PEG 2000 chains through amidic coupling and azide-alkyne click cycloaddition, which we showed as active targeting agents to selectively recognize breast cancer cells such as MCF7 and MDA-MB-231. The GO-PEG-Fol incorporated a high amount of doxorubicin hydrochloride (Doxo) (> 33%) and behaves as NIR-light-activated heater capable of triggering sudden Doxo delivery inside cancer cells and localized hyperthermia, thus provoking efficient breast cancer death. The cytotoxic effect was found to be selective for breast cancer cells, being the IC 50 up to 12 times lower than that observed for healthy fibroblasts. This work established plasma etching as a cost-effective strategy to get functionalized nano-GO with a smart combination of properties such as small size, good photothermal efficiency and targeted cytotoxic effect, which make it a promising candidate as photothermal agent for the treatment of breast cancer.

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supporting

confidence: 78%

Section: Physicochemical Stability Of Go-peg-fol/doxo In Aqueous Mediamentioning

confidence: 99%

Folic acid-functionalized graphene oxide nanosheets via plasma etching as a platform to combine NIR anticancer phototherapy and targeted drug delivery

Mauro

Scialabba

Agnello

et al. 2020

Materials Science and Engineering: C

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“…A significant in vitro cytotoxic effect on breast cancer cells MDA-MB-231 was shown by GO-methyl acrylate (MA) NPs coated with FA and loaded with paclitaxel (PTX). Moreover, in vivo (in DMBA induced breast cancer rats) studies were performed and demonstrated that treatment with this nanosystem regulates the levels of mitochondrial citric acid enzymes to be near normal [159]. Two-dimensional CS polymerized GO and PVP polymerized GO NPs decorated with FA and loaded with camptothecin (CPT) were prepared by Deb and Vimala.…”

Section: Figure 11mentioning

confidence: 99%

Graphenic Materials for Biomedical Applications

2019

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Graphene-based nanomaterials have been intensively studied for their properties, modifications, and application potential. Biomedical applications are one of the main directions of research in this field. This review summarizes the research results which were obtained in the last two years (2017-2019), especially those related to drug/gene/protein delivery systems and materials with antimicrobial properties. Due to the large number of studies in the area of carbon nanomaterials, attention here is focused only on 2D structures, i.e. graphene, graphene oxide, and reduced graphene oxide.

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“…Graphene has also been reported as a promising material for various applications ranging from quantum physics, catalysis, and engineering of nanocomposites nanoelectronics to energy research and biomaterials (14)(15)(16)(17). In a nanomedicine realm, graphene and its composites can be employed in different applications including a new generation of biosensors, nanocarriers for drug delivery, and probes for cell and biological imaging (15,(18)(19)(20). During the last two decades, different nanomaterials of various shapes and chemical compositions, including metal and metal oxide nanoparticles, polymeric micelles, liposomes, dendrimers, and carbon nanotubes, have been studied as nanocarriers for drug delivery (21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

Engineering the pH-Sensitivity of the Graphene and Carbon Nanotube Based Nanomedicines in Smart Cancer Therapy by Grafting Trimetyl Chitosan

et al. 2020

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Purpose The aim of this study was to introduce a smart and responsive drug carrier for Doxorubicin (DOX) and Paclitaxel (PAX) for desirable therapeutic application. Method Loading and releasing of DOX and PAX from smart and pH-sensitive functionalized single-walled carbon nanotube (SWCNTs) and graphene carriers have been simulated by molecular dynamics. The influences of chitosan polymer on proposed carriers have been studied, and both carriers were functionalized with carboxyl groups to improve the loading and releasing properties of the drugs. Results The results showed that DOX could be well adsorbed on both functionalized SWCNTs and graphene. In contrast, there was a weak electrostatic and Van der Waals interaction between both these drugs and carriers at cancerous tissues, which is highly favorable for cancer therapy. Adding trimethyl chitosan (TMC) polymer to carriers facilitated DOX release at acidic tissues. Furthermore, at blood pH, the PAX loaded on the functionalized SWCNTs carrier represented the highest dispersion of the drug while the DOX-graphene showed the highest concentration of the drug at a point. In addition, the mean-square displacement (MSD) results of PAX-graphene indicated that the PAX could be adsorbed quickly and be released slowly. Finally, functionalized graphene-TMC-PAX is a smart drug system with responsive behavior and controllable drug release, which are essential in cancer therapy. Conclusion Simultaneous application of the carboxyl group and TMC can optimize the pH sensitivity of the SWCNTs and graphene to prepare a novel and smart drug carrier for cancer therapy.

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Development of biotin molecule targeted cancer cell drug delivery of doxorubicin loaded κ-carrageenan grafted graphene oxide nanocarrier

Cited by 77 publications

References 39 publications

Folic acid-functionalized graphene oxide nanosheets via plasma etching as a platform to combine NIR anticancer phototherapy and targeted drug delivery

Folic acid-functionalized graphene oxide nanosheets via plasma etching as a platform to combine NIR anticancer phototherapy and targeted drug delivery

Graphenic Materials for Biomedical Applications

Engineering the pH-Sensitivity of the Graphene and Carbon Nanotube Based Nanomedicines in Smart Cancer Therapy by Grafting Trimetyl Chitosan

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