Development of biosensor based on imaging ellipsometry and biomedical applications

Jin, Gang; Meng, Y.H.; Liu, L.; Niu, Yu; Chen, S.; Cai, Qiaoling; Jiang, Taijiao

doi:10.1016/j.tsf.2010.12.175

Cited by 44 publications

(28 citation statements)

References 28 publications

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“…As previously described [41,42], the TIRIE system, which is based on an imaging ellipsometer under the total internal reflection mode, uses the evanescent wave to detect several physical, chemical and biochemical processes at the interfaces, as a real-time and label-free tool operating in a null and off null mode. A SF10 glass slide coated with a thin gold film (30 nm) is used as a substrate for TIRIE biosensing.…”

Section: Total Internal Reflection Imaging Ellipsometrymentioning

confidence: 99%

Nanostructured interfaces with site-specific bioreceptors for immunosensing

Paiva

Almeida

Marquês

et al. 2017

Applied Surface Science

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a b s t r a c tIn this work, we propose a simple and effective approach to build nanostructured immunosensor platforms. The one-step strategy relies on i) the in situ formation of dithiocarbamates from the reaction between carbon disulfide and amine groups, present on protein A, ii) their attachment to gold nanoparticles (AuNPs), and iii) the linkage of the modified AuNPs to the electrode surface, which depends on the strong interaction between gold substrates and sulfur moieties. AuNPs and protein A are used to increase the surface coverage of Immunoglobulin G (IgG) and promote the oriented immobilization of the antibodies on the immunosensing interface. The modified gold surfaces with biomolecules were thoroughly characterized by a combination of techniques: UV-vis spectrophotometry, conventional ellipsometry and atomic force microscopy. The immunosensor performance was assessed in real-time, by surface plasmon resonance and by the highly sensitive total internal reflection imaging ellipsometry, through the specific biorecognition between anti-IgG and the immobilized IgG molecules. We demonstrate that the presence of AuNPs improves the sensitivity of the anti-IgG specific detection, whereas the presence of co-adsorbed CS 2 is responsible for blocking the undesired protein nonspecific adsorption to the gold substrate. Overall, we report a simple and innovative one-step method, to chemically modify gold surfaces with protein A and AuNPs, able to specifically detect antigen/antibody interactions with capability of preventing protein nonspecific adsorption.

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Section: Total Internal Reflection Imaging Ellipsometrymentioning

confidence: 99%

Nanostructured interfaces with site-specific bioreceptors for immunosensing

Paiva

Almeida

Marquês

et al. 2017

Applied Surface Science

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“…The area under the curve with no previous data rejection is 0.842. The ROC analysis has also been practical for other label free biosensors [48][49] . It is important to note that ROC plots are usually applied for the assessment of well-established techniques such as ELISA in the clinical practice.…”

Section: Reliability Of the Immunodetectionmentioning

confidence: 99%

Tackling reproducibility in microcantilever biosensors: a statistical approach for sensitive and specific end-point detection of immunoreactions

Kosaka¹,

Tamayo²,

Ruz³

et al. 2013

Analyst

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aIn the biomedical field, end-point detection bioassays such as enzyme-linked immunosorbent assays (ELISAs) are essential tools because of their simplicity, high-throughput, and suitability for their use at the point-of-care. End-point bioassays are significantly constrained by the need of sample labeling with fluorescent or colorimetric tags for subsequent detection. A promising strategy to overcome these limitations is to harness recent advances in label-free biological nanosensors. Here we analyse the potential of nanomechanical biosensors based on surface stress for the label-free end-point detection of horseradish peroxidase. We address the variability of the sensor responsethrough the analysis of 1012 cantilevers with different antibody surface densities, two blocking strategies based on polyethylene-glycol (PEG) and bovine serum albumin (BSA) and stringent controls. The study reveals that the performance of the assay critically depends on both antibody surface density and blocking strategies. We find that the optimal conditions involve antibody surface densities near but below saturation and blocking with PEG. We find that the surface stress induced by the antibody-antigen binding is significantly correlated to the surface stress generated during the antibody attachment and blocking steps. The statistical correlation is harnessed to identify immobilization failure or success, and thus enhancing the specificity and sensitivity of the assay. This procedure enables achieving a rate of true positives and true negatives of 90% and 91% respectively. The detection limit is of 10 ng/mL (250 pM) that is similar to detection limit obtained in our ELISA

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“…Liu et al applied BTIRIE to visualize the non-specific absorption and release process of vesicles (made of 1,2-Dioleoyl-sn-glycero-3-phosphocholine) from a poly-L-lysine-modified gold surface ( Fig. 11; Jin et al, 2011). Region 6 of curves "a" and "b" in Fig.…”

Section: Btirie Applicationsmentioning

confidence: 99%