Protein microarray biosensors based on imaging ellipsometry techniques and their applications

Abstract

After years of development, biosensors based on imaging ellipsometry and biosensors based on total internal reflection imaging ellipsometry have been successfully implemented in various engineering systems. Their experimental setups, detection principles, and biological and clinical applications are briefly reviewed.

Joint detection of tumor markers with imaging ellipsometry biosensor

“…During the last decade, IEB has been updated and improved in our laboratory [11,12,14]. Now, it is mainly composed of a microfluidic reactor and an imaging ellipsometer.…”

“…With the developments these years, it has become automatic equipment [11,12] for protein interaction analysis. IEB is primarily composed of a microfluidic reactor [13] and an imaging ellipsometer [10,14]. The microfluidic reactor is served to fabricate a patterned protein microarray; while the imaging ellipsometer is used to measure the surface mass concentration distribution of a protein microarray.…”

Binding affinity analysis of the interaction between Homer EVH domain and ryanodine receptor with biosensors based on imaging ellipsometry

“…Imaging ellipsometry has been proposed for inclusion in development of a biosensor to observe biomolecule interactions. 15,16 The biosensor based on imaging ellipsometry (BIE) comprises a micro-uidic reactor and an imaging ellipsometry reader. 17 The micro-uidic reactor injects different liquid solutions on the substrate to construct a patterned biomolecule microarray, while the imaging ellipsometry reader measures surface mass density distribution of the biomolecule microarray.…”

Surface modification methods to improve behavior of biosensor based on imaging ellipsometry

“…For detecting protein interactions with BIE, the general procedure requires the immobilization of ligand on the biosensing interface to form the sensing surface and then the addition of analyte solution to identify the existence of receptors by the specific recognition against ligands [4,17]. Since BIE sensitively responds to even tiny change of layer thickness in the order of 0.1 nm, not only the signal contributed by specific binding but also any undesirable adsorption on the biosensing surface affects BIE result obviously.…”