Development of bioorthogonal SERS imaging probe in biological and biomedical applications

Qiu, Chonggui; Cheng, Ziyi; Lv, Chuanzhu; Wang, Rui; Yu, Fabiao

doi:10.1016/j.cclet.2021.03.016

Cited by 24 publications

(11 citation statements)

References 84 publications

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“…Gold nanoparticles (AuNPs) are at the forefront of optical biosensor development due to their high extinction coefficients, distance-dependent color, fluorescence quenching, localized surface plasmon, and Raman enhancement properties. − Single-stranded DNA oligonucleotides can be adsorbed by AuNPs quickly with a high affinity, while double-stranded or well-folded DNAs are adsorbed at a slower rate. By exploiting this kinetic difference, many detection methods have been achieved using DNA aptamers and DNAzymes. − AuNPs can serve as a quencher in molecular beacons, , and as a barcode for signal generation .…”

Section: Introductionmentioning

confidence: 99%

Stabilization of Gold Nanoparticles by Hairpin DNA and Implications for Label-Free Colorimetric Biosensors

et al. 2022

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With extremely high extinction coefficients and other unique optical properties, gold nanoparticles (AuNPs) have received growing interest in developing biosensors. DNA hairpin structures are very popular probes for the detection of not only complementary DNA or RNA but also aptamer targets. This work aims to understand the effect of the structure and sequence of hairpin DNA for the stabilization of AuNPs and its implications in AuNP-based label-free colorimetric biosensors. A series of hairpin DNA with various loop sizes from 4 to 26 bases and sequences (random sequences, poly-A and poly-T) were tested, but they showed similar abilities to protect AuNPs from aggregation. Using hairpin DNA with a tail under the same conditions, optimal protection was achieved with a six-base or longer tail. DNA hairpins are likely adsorbed via their tail regions or with their terminal bases if no tail is present. Molecular dynamics simulations showed that the rigidity of the hairpin loop region disfavored its adsorption to AuNPs, while the flexible tail region is favored. Finally, a DNA sensing assay was conducted using different structured DNA, where hairpin DNA with a tail doubled the sensitivity compared to the tail-free hairpin.

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Section: Introductionmentioning

confidence: 99%

Stabilization of Gold Nanoparticles by Hairpin DNA and Implications for Label-Free Colorimetric Biosensors

et al. 2022

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“…Surface enhanced Raman scattering spectroscopy is a nondestructive and non-destructive photon scattering technique that provides molecular specific information about the vibrational energy levels of molecules. It is a unique vibrational mode based on the target analyte to provide specific chemical information, and has been widely used in a variety of biomedical detection and imaging [9]. Electromagnetic enhancement mechanism and chemical enhancement mechanism are the two main mechanisms of SERS enhancement.…”

Section: Surface Enhanced Raman Scattering (Sers)mentioning

confidence: 99%

Recent Progress of SERS Nanoprobe for pH Detecting and its Application in Biological Imaging

Zhang¹,

Zhao²,

Jiang³

et al. 2021

Preprint

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pH value almost affects the function of cells and organisms in all aspects, so in biology, biochemical and many other research fields, it is necessary to apply simple, intuitive, sensitive, stable detection of pH and base characteristics inside and outside the cell. Therefore, many research groups have explored the design and application of pH probes based on surface enhanced Raman scattering（SERS）. In this review article, we discussed the basic theoretical background of explaining the working mechanism of pH SERS sensors, and also briefly described the significance of cell pH measurement, and simply classified and summarized the factors that affected the performance of pH SERS probes. Some applications of pH probes based on surface enhanced Raman scattering (SERS) in intracellular and extracellular pH imaging and the combination of other analytical detection techniques are described. And finally, the development prospect of this field is prospected.

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“…14 Furthermore, SERSbased immunoassay technology utilising functional metal nanoparticles has aroused tremendous interest in the early diagnosis of diseases owing to its excellent sensitivity and multiplex detection ability. 18 In our previous work, 19,20 we have successfully fabricated SERS probes based on antibody recognition to conduct sensitive detection of several representative protein biomarkers for the accurate diagnosis of prostate cancer and acute myocardial infarction. These ndings demonstrate that SERS detection technology for rapid and sensitive disease diagnosis tends to be a research focus via rapid, in situ, and nondestructive testing of biomarkers.…”

Section: Introductionmentioning

confidence: 99%

SERS based Y-shaped aptasensor for early diagnosis of acute kidney injury

et al. 2022

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Development of bioorthogonal SERS imaging probe in biological and biomedical applications

Cited by 24 publications

References 84 publications

Stabilization of Gold Nanoparticles by Hairpin DNA and Implications for Label-Free Colorimetric Biosensors

Stabilization of Gold Nanoparticles by Hairpin DNA and Implications for Label-Free Colorimetric Biosensors

Recent Progress of SERS Nanoprobe for pH Detecting and its Application in Biological Imaging

SERS based Y-shaped aptasensor for early diagnosis of acute kidney injury

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