Development of biodegradable membranes for the delivery of a bioactive chitosan‐derivative on cartilage defects: A preliminary investigation

Abstract

Biodegradable membranes for cartilage applications were manufactured starting from polymeric networks of a lactose-modified chitosan (CTL), previously proposed for chondrocytes stimulation. This implantable biomaterial was conceived as a reservoir of a bioactive polymer that could promote the activity of chondrocytes and the

A hydrogel system based on a lactose-modified chitosan for viscosupplementation in osteoarthritis

“…Chondrocyte cells were obtained from knee-articular cartilage harvested from adult pigs kindly provided by a slaughterhouse, following a procedure previously described (Scognamiglio, Travan, Borgogna, Donati, & Marsich, 2020). Thin slices of cartilage tissue were cut and incubated with a solution containing hyaluronidase (270 U/mL), penicillin (500 U/mL) and streptomycin (500 U/mL).…”

“…Lactate dehydrogenase (LDH) assay was performed on primary chondrocytes isolated from pig's articular cartilage and on an osteoblast cell line (Osteosarcoma MG-63 cell line, ATCC number: CRL-1427) to evaluate in vitro the biocompatibility of C3-B2 hydrogels after autoclave (C3-B2 AC), following a protocol reported in the literature with slight modifications (Scognamiglio et al, 2020). The cells were cultured in cell medium (DMEM) supplemented with FBS 10 % and penicillin/ streptomycin 0.25 %, at 37 °C and 5% CO 2 .…”

Bioactive Lactose-Modified Chitosan Acts as a Temporary Extracellular Matrix for the Formation of Chondro-Aggregates

“…In this way, the expression of Gal-1-regulated genes is reduced, leading to a decrease in inflammation . Since CTL has been demonstrated to be able to inhibit the expression of galectin proteins both in vivo and in vitro , ,,, there is a growing interest in the use of this polymer for the production of materials to be used in the field of cartilage regeneration. , …”

Antibacterial Electrospun Polycaprolactone Membranes Coated with Polysaccharides and Silver Nanoparticles for Guided Bone and Tissue Regeneration

“…In these devices, PCL is usually the main component because of the abovementioned advantages and is often combined with gelatin or other polymers in order to increase its bioactivity and modulate its degradation rate. ,− In the context of bioactive polymer adsorption, lactose-modified chitosan (CTL) is a promising polysaccharide, which can be used to functionalize biomaterials. CTL is derived from the addition of lactose moieties to the chitosan backbone; the structure thus obtained is highly hydrophilic and can be used for the preparation of coatings, , viscous solutions and hydrogels, − membranes, , and nanoparticles . The bioactivity of CTL is mediated by galectins, which are proteins present in the intracellular and extracellular environments that trigger molecular signaling when they bind specific residues as galactose moieties. , The first evidence of CTL bioactivity was shown for chondrocytes: CTL induces their aggregation and stimulates the synthesis of the extracellular matrix and glycosaminoglycan .…”

“…5,25−27 In the context of bioactive polymer adsorption, lactose-modified chitosan (CTL) is a promising polysaccharide, which can be used to functionalize biomaterials. CTL is derived from the addition of lactose moieties to the chitosan backbone; the structure thus obtained is highly hydrophilic and can be used for the preparation of coatings, 28,29 viscous solutions and hydrogels, 30−32 membranes, 33,34 and nanoparticles. 35 The bioactivity of CTL is mediated by galectins, which are proteins present in the intracellular and extracellular environments that trigger molecular signaling when they bind specific residues as galactose moieties.…”