Development of biocompatible and VEGF-targeted paclitaxel nanodrugs on albumin and graphene oxide dual-carrier for photothermal-triggered drug delivery in vitro and in vivo

Abstract

In this study, we performed the characterization and synthesis of biocompatible and targeted albumin and graphene oxide (GO) dual-carrier paclitaxel (PTX) nanoparticles for photothermal-triggered tumor therapy. PTX absorbed on GO nanosheets as cores were coated with human serum albumin (HSA), following surface conjugation with monoclonal antibodies (mAb) against vascular endothelial growth factor (VEGF; denoted as mAbVEGF) via polyethylene glycol linker to form targeted nanoparticles (PTX-GHP-VEGF). The spherical nanoparticles were 191±5 nm in size with good stability and biocompatibility. GO functioned as the first carrier and a near infrared absorber that can generate photothermal effects under 5-minute 808-nm laser irradiation to thermal trigger the release of PTX from the second carrier HSA nanoparticles. The mechanism of thermal-triggered drug release was also investigated preliminarily, in which the heat generated by GO induced swelling of PTX-GHP-VEGF nanoparticles which released the drugs. In vitro studies found that PTX-GHP-VEGF can efficiently target human SW-13 adrenocortical carcinoma cells as evaluated by confocal fluorescence microscopy as well as transmission electron microscopy, and showed an obvious thermal-triggered antitumor effect, mediated by apoptosis. Moreover, PTX-GHP-VEGF combined with near infrared irradiation showed specific tumor suppression effects with high survival rate after 100 days of treatment. PTX-GHP-VEGF also demonstrated high biosafety with no adverse effects on normal tissues and organs. These results highlight the remarkable potential of PTX-GHP-VEGF in photothermal controllable tumor treatment.

<p>Promoting tendon to bone integration using graphene oxide-doped electrospun poly(lactic-co-glycolic acid) nanofibrous membrane</p>

“…Previous studies reported that GO exhibits the ability to allow for the migration and growth of osteoblasts and mesenchymal stem cells and had a greater osteoconductivity ability compared with traditional calcium phosphate ceramics. [47][48][49] Therefore, in this study we fabricated the combined nanofibrous scaffold (GO-PLGA) by the electrospinning technique. GO has received enormous attention in the bone tissue engineering field due to its unique sp 2 carbon domains, large surface area, and hydrophilic functional group structure.…”

<p>Paclitaxel/IR1061-Co-Loaded Protein Nanoparticle for Tumor-Targeted and pH/NIR-II-Triggered Synergistic Photothermal-Chemotherapy</p>

“…According to literature reports, 36,44 FITC was used to label IR-AFN@PTX and IR-AFN@PTX-FA by physical adsorption. Treat 4T1 cells with FITC-labeled IR-AFN @PTX and IR-AFN@PTX-FA for 5 hrs, then stain with DAPI and lysosomal-specific dye LysoTracker, and observe IR-AFN@PTX and IR-AFN@PTX-FA in cells using commercial LSM.…”

“…34,35 Paclitaxel (PTX) is an FDA-approved chemotherapy with fewer side effects and has a high inhibitory effect on a variety of malignant tumors. 36,37 However, its solubility in water is poor and requires delivery in a solution of Cremophor EL and ethanol for clinical use. 38 Cremophor EL has been reported to have serious side effects such as hypersensitivity reactions and acute renal impairment.…”

Graphene and Graphene‐Based Materials in Biomedical Science

“…Super‐paramagnetic iron oxide‐reduced graphene oxide nanohybrid has been used as a vehicle for targeted drug delivery and hyperthermia treatment of cancer . VEGF‐targeted paclitaxel nanodrugs on albumin and graphene oxide have been used as a dual‐carrier for photothermal‐triggered drug delivery in vitro and in vivo in human SW‐13 adrenocortical carcinoma cells http://Chitosan. functionalized graphene nanobiocomposite has been fabricated for the codelivery of two anticancer drugs called 3,3′‐diindolylmethane and CPT.…”