Development of Bacterial Biofilms on Silastic Catheter Materials in Peritoneal Dialysis Fluid

Dasgupta, Mrinal; Ward, Kathy; Noble, Peter A.; Larabie, M; Costerton, J. William

doi:10.1016/s0272-6386(12)70281-0

Cited by 39 publications

(34 citation statements)

References 16 publications

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“…The initial objectives of these in vitro systems were to test whether bacterial strains associated with clinical peritonitis ( S. epidermis , S. aureus , E. coli , Pseudomonas ) can actually grow in biofilms on silastic catheter materials in an experimental PD environment. Experiments done in a MRD confirmed that S. epidermidis adheres to and grows easily, forming mature biofilm on silastic catheter implants in PD effluents, but not in fresh PD solution (42). Pseudomonas also formed confluent and mature biofilms on silastics, but in the same PD environment and time period, E. coli grew the least in biofilm (42).…”

Section: Common Bacteria Adheres To Silastic Catheters and Form A Biomentioning

confidence: 97%

Biofilms and Infection in Dialysis Patients

Dasgupta

2002

Seminars in Dialysis

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Biofilm bacterial infections are implicated in most human bacterial infections and are also common in patients undergoing treatment with hemodialysis and peritoneal dialysis. Skin bacteria, which grow into microcolonies with biofilm formation in dialysis environments, are implicated in most of these infections. Dissemination of bacterial biofilms in hemodialysis patients induces bacteremia and endotoxemia. In peritoneal dialysis patients, biofilm causes peritonitis and catheter-related infections with consequent loss of catheters and technique failure. Effective strategies for the diagnosis, intervention, and prevention of biofilm-related infections in dialysis patients are described in this review.

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Section: Common Bacteria Adheres To Silastic Catheters and Form A Biomentioning

confidence: 97%

Biofilms and Infection in Dialysis Patients

Dasgupta

2002

Seminars in Dialysis

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“…The study of biofilm eradication activity involved biofilm colonization of silicone coupon surfaces (diameter, 0.8 cm; thickness, 1 mm; NuSil 4970) in modified Robbins device flow cells. Detailed information on the Robbins device can be found elsewhere (6). Silicone coupons were sealed into coupon housings with sealant (Dow Corning 732 silicone; Dow Corning, Midland, Mich.).…”

Section: Biofilm Eradication Activity (I) Test Organismsmentioning

confidence: 99%

Antimicrobial Activity of a Novel Catheter Lock Solution

Shah

Mittelman²,

Costerton

et al. 2002

Antimicrob Agents Chemother

175

129

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Intravascular catheter-associated bloodstream infections significantly increase rates of morbidity and hospital costs. Microbial colonization and development of biofilms, which are known to be recalcitrant to antibiotic therapy, often lead to the loss of otherwise patent vascular access systems. We evaluated a new taurolidine-and citrate-based catheter lock solution (Neutrolin; Biolink Corporation, Norwell, Mass.) for its activity against planktonic microbes, antimicrobial activity in a catheter model, and biofilm eradication activity. In studies of planktonic microbes, after 24 h of contact, 675 mg of taurolidine-citrate solution per liter caused >99% reductions in the initial counts of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Entercoccus faecalis. A solution of 13,500 mg/liter was cidal for Candida albicans. Ports and attached catheters inoculated with 50 to 600 CFU of these bloodstream isolates per ml were locked with heparin or the taurolidine-citrate solution. After 72 h, there was no growth in the taurolidine-citrate-treated devices but the heparin-treated devices exhibited growth in the range of 6 ؋ 10 2 to 5 ؋ 10 6 CFU/ml. Biofilms were developed on silicone disks in modified Robbins devices with broth containing 6% serum (initial counts, 10 6 to 10 8 CFU/cm 2 ). The axenic biofilms were treated for 24 h with taurolidine-citrate or heparin. Taurolidine-citrate exposure resulted in a median reduction of 4.8 logs, whereas heparin treatment resulted in a median reduction of 1.7 logs (P < 0.01). No significant differences in the effects of the two treatments against P. aeruginosa and C. albicans were observed. These findings suggest that taurolidine-citrate is a promising combination agent for the prevention and treatment of intravascular catheter-related infections.

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“…Another reason could be the shorter recommended duration of antibiotic therapy for CNS peritonitis in some centers (2 weeks for CNS vs. 3 weeks for other organisms) and the lower catheter removal rate for CNS infections compared to S. aureus peritonitis, both due to the lesser severity and more prompt response to therapy of the former infection [23,34]. Additionally, CNS may have a propensity to colonize catheters with biofilms, with some limited data available to support the contention that CNS is different from other organisms in this regard [36]. Nodaira et al investigated risk factors and causes of PD catheter removal in 63 patients receiving CAPD and showed that all catheters removed because of repeat peritonitis had an accompanying biofilm detected upon electron microscopic examination, whereas catheters removed for other reasons (e.g.…”

Section: Discussionmentioning

confidence: 99%

Difficult peritonitis cases in children undergoing chronic peritoneal dialysis: relapsing, repeat, recurrent and zoonotic episodes

Bakkaloğlu

Warady

2014

Pediatr Nephrol

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Despite technological improvements in dialysis connectology and dialysis technique, peritonitis remains the most common and most significant complication of peritoneal dialysis (PD) in children. Most children undergoing chronic PD experience none or only one peritonitis episode, while others have multiple episodes or episodes secondary to unusual organisms. Knowledge of potential risk factors and likely patient outcome is imperative if treatment is to be optimized. In this review we will, in turn, describe episodes of peritonitis that are characterized as either relapsing, recurrent, repeat or zoonosis-related to highlight the clinical issues that are commonly encountered by clinicians treating these infections.

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Development of Bacterial Biofilms on Silastic Catheter Materials in Peritoneal Dialysis Fluid

Cited by 39 publications

References 16 publications

Biofilms and Infection in Dialysis Patients

Biofilms and Infection in Dialysis Patients

Antimicrobial Activity of a Novel Catheter Lock Solution

Difficult peritonitis cases in children undergoing chronic peritoneal dialysis: relapsing, repeat, recurrent and zoonotic episodes

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