Development of aqueous mobile phase using chaotrope for the chromatographic determination of melamine in infant formula

Suh, Joon Hyuk; Jung, Juhee; Kim, Byeong-Hee; Cho, Hyun-Deok; Kim, Jung-Hyun; Eom, Taeyong; Park, Mi-Hee; Wang, Yu; Han, Sang Beom

doi:10.1016/j.chroma.2017.03.045

Cited by 4 publications

(1 citation statement)

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“…According to literature, a good number of compounds such as thiamine, urazamide, and melamine were successfully determined using HPLC with room‐temperature ionic liquid (RTIL)‐modified mobile phase . A multitude of HPLC methods have been developed for determining or screening β‐lactams in pharmaceuticals, food, or biological samples .…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an HPLC Method Using a Less Hazardous Mobile Phase for the Determination of some β‐Lactams

Pham

Mai

et al. 2019

Bulletin Korean Chem Soc

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A simple, cost-effective, and greener HPLC method was investigated to determine 14 β-lactams. To achieve this, various strategies were implemented such as the use of an aqueous mobile phase, ionic liquids, or an alternative organic solvent. Preliminary experiments were conducted using "one-factor-at-atime" to identify the significant factors affecting chromatographic performance. The experimental design (central composite design) was then applied to thoroughly examine the effects of the key factors and exploit them to achieve optimal HPLC condition. The developed method was less hazardous and improved the retention and peak shape, compared with other conventional methods. The optimized assay condition was validated according to the ICH guidelines and effectively applied for the quantification of some β-lactams in various formulations. These results imply that this novel HPLC method can be employed for routine analyses of some β-lactams in pure drug and commercial formulations.

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Section: Introductionmentioning

confidence: 99%