Development of analytical method for catechol compounds in mouse urine using hydrophilic interaction liquid chromatography with fluorescence detection

Kanamori, Takashi; Isokawa, Muneki; Funatsu, Takashi; Tsunoda, Makoto

doi:10.1016/j.jchromb.2015.01.038

Cited by 42 publications

(21 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The determination of PHPLA, PHPAA and PHPA were mainly carried out by HPLC methods, such as HPLC-UV [20,21], HPLC with fluorescence detection (FLD) [22][23][24] and HPLC-MS [25][26][27]. Among them, the sensitivity of UV method is low, therefore the UV method is not suitable for the determination of trace amount of tyrosine metabolites in urine; MS method is sensitive, but the MS equipment is expensive [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 4-hydroxyphenyl lactic acid, 4-hydroxyphenyl acetic acid, and 3,4-hydroxyphenyl propionic acid in human urine by ultra-high performance liquid chromatography with fluorescence detection

2017

View full text Add to dashboard Cite

A simple and reliable method was established for simultaneous determination of 4-hydroxyphenyl acetic acid, 4-hydroxyphenyl lactic acid, and 3,4-hydroxyphenyl propionic acid in human urine by high-performance liquid chromatography with fluorescence detection. Solid-phase extraction was used to eliminate the interferences in urine. The separation of three analytes was achieved using a C column and a mobile phase formed by a 95:5 v/v mixture of 50 mmol/L ammonium acetate buffer at pH 6.8 that contained 5 mmol/L tetrabutyl ammonium bromide and acetonitrile. Under the optimized conditions, the detection limits of 4-hydroxyphenyl acetic acid, 4-hydroxyphenyl lactic acid, and 3,4-hydroxyphenyl propionic acid were 4.8 × 10 , 8.80 × 10 , and 9.00 × 10 mg/L, respectively, and the recoveries were in the range of 85.0-120.0% with relative standard deviations of 1.5-3.1%. This method was used to analyze urine samples from breast cancer patients, healthy people and post-surgery breast cancer patients. Significant differences in urinary levels of 4-hydroxyphenyl acetic acid and 4-hydroxyphenyl lactic acid could be found between the breast cancer patients group and other two groups. No effect of age and sex was observed on the urinary levels of 4-hydroxyphenyl acetic acid and 4-hydroxyphenyl lactic acid. This method might be helpful for cancer biomarkers discovery in urine.

show abstract

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of 4-hydroxyphenyl lactic acid, 4-hydroxyphenyl acetic acid, and 3,4-hydroxyphenyl propionic acid in human urine by ultra-high performance liquid chromatography with fluorescence detection

2017

View full text Add to dashboard Cite

show abstract

“…In a previous study, we developed a method for analyzing catechol compounds in HILIC [11]. Catechol compounds (dopamine (DA), epinephrine (E), norepinephrine (NE), 3,4-dihydroxyphenylalanine (DOPA), 3,4-dihydroxyphenylacetic acid (DOPAC), 3,4-dihydroxyphenylglycol (DHPG), and 3,4-dihydroxymandelic acid (DHMA)) were successfully separated using a ZIC-cHILIC column containing a phosphorylcholine moiety on the stationary phase.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Effects of Sample Solutions and Injector Wash Solutions on Separation Efficiency in Hydrophilic Interaction Liquid Chromatography

2015

Self Cite

View full text Add to dashboard Cite

Hydrophilic interaction liquid chromatography (HILIC) is advantageous for separation of polar compounds and sensitive detection in mass spectrometry. Sample dilution can negatively affect separation. We previously reported that separation efficiency in HILIC is affected by sample water content and perhaps the wash solution used in the injector sample loop. In this study, the effects of sample composition, sample volume, and wash solutions, on separation in HILIC was investigated using a mixture of catechol compounds including basic, acidic, and zwitterionic analogs. A ZIC-cHILIC column with a phosphorylcholine stationary phase was used for analysis. In the investigation of the effect of sample composition and volume, it was shown that separation efficiency was dependent on the amount of water injected into the column. Furthermore, we found that the most distorted peaks had similar elution times to water. We also investigated the effect of the sample loop wash solution, with separation efficiency reduced by increasing water content in the wash solutions. These results indicated that the water content of both sample and washing solutions should be kept to a minimum in HILIC separation.

show abstract

“…[11][12][13][14] On this basis, we therefore supposed that HILIC may be suitable for the separation of hydrophilic catechol compounds and accordingly developed a HILIC method with fluorescence detection for determination of catechol compounds in mouse urine. 15 The new method allowed the separation of eight catechol compounds within 15 min. Furthermore, high sensitivity was realized without the use of fluorescence derivatization, probably because of an enhancement in the fluorescence intensity in the acetonitrile-rich mobile phase used for HILIC separation.…”

Section: Introductionmentioning

confidence: 99%

“…We have previously used selective SPE with a phenylboronic acid (PBA)modified spin column as a pretreatment for samples containing catechol compounds. 15,17,18 PBA forms a negatively charged complex with the cis-hydroxy groups of catechol compounds in basic conditions. The bound catechol compounds can be subsequently released by lowering the pH with an acidic media.…”

Section: Introductionmentioning

confidence: 99%

Determination of catecholamines and related compounds in mouse urine using column-switching HPLC

Kanamori

Funatsu

Tsunoda

2016

Analyst

Self Cite

View full text Add to dashboard Cite

We have developed an analytical method for the determination of catecholamines and related compounds in mouse urine by column-switching HPLC. Selective extraction of the catechol compounds was performed using a precolumn modified with phenylboronic acid, which has a pH dependent affinity for the catechol structures. The pretreatment buffer, which facilitated binding of the catechols to the precolumn, was optimized to ensure high analyte recoveries and good peak shapes. We found that using the same acetonitrile content in the pretreatment buffer and hydrophilic interaction liquid chromatography mobile phase was necessary to improve peak shapes. Eight catechol compounds were selectively extracted and separated using 100 mmol L(-1) ammonium formate/acetonitrile (20/80 v/v, pH 8.0) for the extraction step, and 20 mmol L(-1) ammonium formate (pH 2.5)/acetonitrile (20/80 v/v) for elution and separation. Native fluorescence of the separated catechol compounds was monitored, and the limits of detection, corresponding to a signal to noise ratio of 3, were 9-58 nmol L(-1). Five catechol compounds (dopamine, epinephrine, norepinephrine, 3,4-dihydroxyphenylglycol, and 3,4-dihydroxymandelic acid) were successfully quantified in mouse urine. Intra- and inter-day precisions were less than 10%, and performance was superior to that afforded by manual sample pretreatment.

show abstract

Development of analytical method for catechol compounds in mouse urine using hydrophilic interaction liquid chromatography with fluorescence detection

Cited by 42 publications

References 26 publications

Simultaneous determination of 4-hydroxyphenyl lactic acid, 4-hydroxyphenyl acetic acid, and 3,4-hydroxyphenyl propionic acid in human urine by ultra-high performance liquid chromatography with fluorescence detection

Simultaneous determination of 4-hydroxyphenyl lactic acid, 4-hydroxyphenyl acetic acid, and 3,4-hydroxyphenyl propionic acid in human urine by ultra-high performance liquid chromatography with fluorescence detection

Evaluation of the Effects of Sample Solutions and Injector Wash Solutions on Separation Efficiency in Hydrophilic Interaction Liquid Chromatography

Determination of catecholamines and related compounds in mouse urine using column-switching HPLC

Contact Info

Product

Resources

About