Development of an X-ray pixel detector with multi-port charge-coupled device for X-ray free-electron laser experiments

Kameshima, Takashi; Ono, S.; Kudo, Togo; Ozaki, Kyosuke; Kirihara, Yoichi; Kobayashi, Kazuo; Inubushi, Yuichi; Yabashi, Makina; Horigome, Toshio; Holland, Andrew D.; Holland, Karen; Burt, David; Murao, Hajime; Hatsui, Takaki

doi:10.1063/1.4867668

Cited by 241 publications

(189 citation statements)

References 43 publications

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“…RNAi microsponges randomly mounted on the membrane were exposed to XFEL pulses by scanning the focused X-ray beams across the membrane window. The resulting diffraction patterns were recorded on a multi-port CCD detector 46 . The multi-port CCD is assembled with eight sensors to give a 2,048 Â 2,048 array with 50 Â 50 mm 2 size pixels with a 3 Â 3-mm 2 central aperture passing the direct beam.…”

Section: Methodsmentioning

confidence: 99%

Macromolecular structures probed by combining single-shot free-electron laser diffraction with synchrotron coherent X-ray imaging

et al. 2014

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Nanostructures formed from biological macromolecular complexes utilizing the self-assembly properties of smaller building blocks such as DNA and RNA hold promise for many applications, including sensing and drug delivery. New tools are required for their structural characterization. Intense, femtosecond X-ray pulses from X-ray free-electron lasers enable single-shot imaging allowing for instantaneous views of nanostructures at ambient temperatures. When combined judiciously with synchrotron X-rays of a complimentary nature, suitable for observing steady-state features, it is possible to perform ab initio structural investigation. Here we demonstrate a successful combination of femtosecond X-ray single-shot diffraction with an X-ray free-electron laser and coherent diffraction imaging with synchrotron X-rays to provide an insight into the nanostructure formation of a biological macromolecular complex: RNA interference microsponges. This newly introduced multimodal analysis with coherent X-rays can be applied to unveil nano-scale structural motifs from functional nanomaterials or biological nanocomplexes, without requiring a priori knowledge.

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Section: Methodsmentioning

confidence: 99%

Macromolecular structures probed by combining single-shot free-electron laser diffraction with synchrotron coherent X-ray imaging

et al. 2014

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“…[43]. Figure 1 shows a standard setup of DAPHNIS, which consists of a sample chamber, microscopes for sample monitoring, and a multi-port charge-coupled-device (MPCCD) detector with eight sensor modules [44] . The chamber is filled with He gas, a partial pressure of which is kept higher than 0.9 atm during measurement.…”

Section: Daphnis For Sfxmentioning

confidence: 99%

Fluid sample injectors for x-ray free electron laser at SACLA

Tono

2017

High Pow Laser Sci Eng

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This paper provides a review on sample injectors which are provided at SPring-8 Angstrom Compact free electron LAser (SACLA) for conducting serial measurement in a 'diffract-before-destroy' scheme using an x-ray free electron laser (XFEL). Versatile experimental platforms at SACLA are able to accept various types of injectors, among which liquidjet, droplet and viscous carrier injectors are frequently utilized. These injectors produce different forms of fluid targets such as a liquid filament with a diameter in the order of micrometer, micro-droplet synchronized to XFEL pulses, and slowly flowing column of highly viscous fluid with a rate below 1 µL min −1 . Characteristics and applications of the injectors are described.

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“…1). Raw data from eight multi-port charge coupled device (MPCCD) sensor modules (Kameshima et al, 2014) are captured by frame grabbers and transferred to data handling servers. These servers temporarily buffer images and write all of them to the cache storage.…”

Section: Online and Offline Apimentioning

confidence: 99%

“…The sensor-specific gain is measured by the detector team [see the Calibration section in the paper by Kameshima et al (2014)] and is available from the SACLA API. This normalization makes the spot finding parameters less sensitive to the photon energy.…”

Section: Image Conversionmentioning

confidence: 99%

Data processing pipeline for serial femtosecond crystallography at SACLA

et al. 2016

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A data processing pipeline for serial femtosecond crystallography at SACLA was developed, based on Cheetah [Barty et al. (2014). J. Appl. Cryst. 47, 1118-1131] and CrystFEL [White et al. (2016). J. Appl. Cryst. 49, 680-689]. The original programs were adapted for data acquisition through the SACLA API, thread and inter-node parallelization, and efficient image handling. The pipeline consists of two stages: The first, online stage can analyse all images in real time, with a latency of less than a few seconds, to provide feedback on hit rate and detector saturation. The second, offline stage converts hit images into HDF5 files and runs CrystFEL for indexing and integration. The size of the filtered compressed output is comparable to that of a synchrotron data set. The pipeline enables real-time feedback and rapid structure solution during beamtime.

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Development of an X-ray pixel detector with multi-port charge-coupled device for X-ray free-electron laser experiments

Cited by 241 publications

References 43 publications

Macromolecular structures probed by combining single-shot free-electron laser diffraction with synchrotron coherent X-ray imaging

Macromolecular structures probed by combining single-shot free-electron laser diffraction with synchrotron coherent X-ray imaging

Fluid sample injectors for x-ray free electron laser at SACLA

Data processing pipeline for serial femtosecond crystallography at SACLA

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