Development of an odorant sensor with a cell-free synthesized olfactory receptor and a graphene field-effect transistor

Yoshii, Tomoya; Takayama, Ikumi; Fukutani, Yosuke; Ikuta, Takashi; Maehashi, Kenzo; Yohda, Masafumi

doi:10.1007/s44211-022-00073-y

Cited by 5 publications

(2 citation statements)

References 32 publications

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“…In addition, graphene can be modified with organic molecules on its surface via π-stacking, owing to its large π-conjugated system, which is not found in other inorganic semiconductor materials [ 20 , 21 , 22 , 23 ]. By taking advantage of these features, previous studies have presented graphene-based sensors optimized for various target molecules, such as antibodies, acid gases, and organic molecules [ 24 , 25 , 26 , 27 ]. In graphene-based sensors, the choice of the modified molecule on graphene is key to providing the ability to capture the target molecule.…”

Section: Introductionmentioning

confidence: 99%

Identification of Cadmium Compounds in a Solution Using Graphene-Based Sensor Array

Yoshii

Nishitsugu

Kikawada

et al. 2023

Sensors

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Rapid detection of heavy metals in solution is necessary to ensure human health and environmental protection. Some heavy-metal compounds are present in solution as compounds instead of as ions owing to their low ionization. Therefore, the development of sensor devices for the detection of heavy-metal compounds is important. In this study, as a proof of concept, we propose a sensor device using graphene and a chelating agent, which were used to develop an identification technique for three types of cadmium compounds. Pristine-graphene and two types of chelator-modified graphene-based sensors were successfully used to detect cadmium compounds at concentrations ranging from 50 to 1000 μM. The detection time was less than 5 min. The three type of graphene-based sensors responded differently to each cadmium compound, which indicates that they detected cadmium as a cadmium compound instead of as cadmium ions. Furthermore, we successfully identified cadmium compounds by operating these three types of sensors as a sensor array on the same substrate. The results indicate that sensors that focus on heavy-metal compounds instead of heavy-metal ions can be used for the detection of heavy metals in solution.

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Section: Introductionmentioning

confidence: 99%

Identification of Cadmium Compounds in a Solution Using Graphene-Based Sensor Array

Yoshii

Nishitsugu

Kikawada

et al. 2023

Sensors

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“… 1, Wetzel et al (1999) ; 2, Wanner et al (2007) ; 3, Hou et al (2020) ; 4, Parker et al (1994) ; 5, Anderson et al (2009) ; 6, Claudianos et al (2014) ; 7, Grosse-Wilde et al (2006) ; 8, Corcoran et al (2014) ; 9, Hamana et al (2010) ; 10, Gonzalez-Kristeller et al (2015) ; 11, Dobritsa et al (2003) ; 12, Hallem et al (2004) ; 13, Kurtovic et al (2007) ; 14, Montagné et al (2012) ; 15, de Fouchier et al (2017) ; 16, Chahda et al (2019) ; 17, Tegler et al (2015) ; 18, Misawa et al (2019) ; 19, Zhao et al (1998) ; 20, Shirokova et al (2005) ; 21, Krautwurst (2008) ; 22, Lundin et al (2007) ; 23, Sakurai et al (2011) ; 24, Tsitoura et al (2010) ; 25, Kaiser et al (2008) ; 26, Yoshii et al (2022) ; 27, Thach et al (2017) ; 28, Levasseur et al (2003) ; 29, Levasseur et al (2004) ; 30, Ritz et al (2013) . …”

Section: Introductionunclassified

Transcuticular calcium imaging as a tool for the functional study of insect odorant receptors

Mariette,

Noël,

Louis

et al. 2023

Front. Mol. Neurosci.

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The primary actors in the detection of olfactory information in insects are odorant receptors (ORs), transmembrane proteins expressed at the dendrites of olfactory sensory neurons (OSNs). In order to decode the insect olfactome, many studies focus on the deorphanization of ORs (i.e., identification of their ligand), using various approaches involving heterologous expression coupled to neurophysiological recordings. The “empty neuron system” of the fruit fly Drosophila melanogaster is an appreciable host for insect ORs, because it conserves the cellular environment of an OSN. Neural activity is usually recorded using labor-intensive electrophysiological approaches (single sensillum recordings, SSR). In this study, we establish a simple method for OR deorphanization using transcuticular calcium imaging (TCI) at the level of the fly antenna. As a proof of concept, we used two previously deorphanized ORs from the cotton leafworm Spodoptera littoralis, a specialist pheromone receptor and a generalist plant odor receptor. We demonstrate that by co-expressing the GCaMP6s/m calcium probes with the OR of interest, it is possible to measure robust odorant-induced responses under conventional microscopy conditions. The tuning breadth and sensitivity of ORs as revealed using TCI were similar to those measured using single sensillum recordings (SSR). We test and discuss the practical advantages of this method in terms of recording duration and the simultaneous testing of several insects.

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Recent development of electrochemical devices in analytical chemistry

Ino

2023

ANAL. SCI.

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Development of an odorant sensor with a cell-free synthesized olfactory receptor and a graphene field-effect transistor

Cited by 5 publications

References 32 publications

Identification of Cadmium Compounds in a Solution Using Graphene-Based Sensor Array

Identification of Cadmium Compounds in a Solution Using Graphene-Based Sensor Array

Transcuticular calcium imaging as a tool for the functional study of insect odorant receptors

Recent development of electrochemical devices in analytical chemistry

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