Development of an innovative maceration technique to optimize extraction and phase partition of natural products

Gori, Anthonin; Boucherle, Benjamin; Rey, Aurélien; Rome, Maxime; Fuzzati, Nicola; Peuchmaur, Marine

doi:10.1016/j.fitote.2020.104798

Cited by 19 publications

(10 citation statements)

References 11 publications

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“…The triphasic extraction had the advantage of mixing five solvents with different polarities for a single extraction step, with a consequent reduction in process time and solvent consumption. The solvents used for the TE were chosen on the bases of the results reported in the paper by Gori et al [ 27 ]. The yields of extraction of the apolar phase, intermediate phase and polar phase were 13%, 5% and 8%, respectively, and were composed largely of fatty materials, secondary metabolites and sugars, respectively, as showed by NMR spectra.…”

Section: Resultsmentioning

confidence: 99%

“…In the acetone/ethyl acetate extraction (AAE), grapefruit seed powder (3 g) was extracted with a mixture 1:1 (10 mL × 2) at rt in the dark for 48 h. The extracts were filtered and evaporated under reduced pressure. In the triphasic extraction (TE), grapefruit seed powder (3 g) was extracted with a system of five solvents with different polarities (n-heptane/EtOAc/ACN/ButOH/H 2 O with proportion 22:14:29:8:27) [ 27 ]. The mixture was stirred for 14 h at 35 °C and decanted.…”

Section: Methodsmentioning

confidence: 99%

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Antioxidant Activity of Citrus Limonoids and Investigation of Their Virucidal Potential against SARS-CoV-2 in Cellular Models

et al. 2021

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The COVID-19 pandemic represents an unprecedented global emergency. Despite all efforts, COVID-19 remains a threat to public health, due to the complexity of mass vaccination programs, the lack of effective drugs, and the emergence of new variants. A link has recently been found between the risk of developing a severe COVID-19 infection and a high level of oxidative stress. In this context, we have focused our attention on natural compounds with the aim of finding molecules capable of acting through a dual virucidal–antioxidant mechanism. In particular, we studied the potential of grapefruit seed extracts (GSE) and their main components, belonging to the class of limonoids. Using chemical and biological approaches including isolation and purification of GSE, antioxidant and virucidal assays, we have shown that grapefruit seed constituents, belonging to the class of limonoids, are endowed with remarkable virucidal, antioxidant and mitoprotective activity.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Antioxidant Activity of Citrus Limonoids and Investigation of Their Virucidal Potential against SARS-CoV-2 in Cellular Models

et al. 2021

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“…Extractions of increasing polarities were performed by three sequential maceration following an experimental procedure adapted from Gori et al [ 18 ] using three solvent mixtures: heptane/ethyl acetate/acetonitrile/butan-1-ol (87:6:5:2) (% volume) for the apolar phase, heptane/ethyl acetate/acetonitrile/butan-1-ol/water (4:21:43:13:19) for the intermediate phase and ethyl acetate/acetonitrile/butan-1-ol/water (6:28:6:60) for the polar phase. The powdered aerial plant material was placed in a 2 L round bottom flask.…”

Section: Methodsmentioning

confidence: 99%

Investigation of Chemical Composition and Biological Activities of Ajuga pyramidalis—Isolation of Iridoids and Phenylethanoid Glycosides

Gori

Boucherle

Rey³

et al. 2023

Metabolites

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Despite several studies on the Ajuga L. genus, the chemical composition of Ajuga pyramidalis, an alpine endemic species, is still largely unknown. The purpose of this study was to therefore deeper describe it, particularly from the phytochemistry and bioactivity perspectives. In that respect, A. pyramidalis was investigated and 95% of the extracted mass of the plant was characterized by chromatography and mass spectrometry. Apart from the already determined chemical compounds, namely, harpagide and 8-O-acetylharpagide, two iridoids, and neoajugapyrin A, a neo-clerodane diterpene, and three polyphenols (echinacoside, verbascoside and teupoloside) were identified for the first time in A. pyramidalis. Incidentally, the first RX structure of a harpagoside derivative is also described in this paper. The extracts and isolated compounds were then evaluated for various biochemical or biological activities; notably a targeted action on the renewal of the epidermis was highlighted with potential applications in the cosmetic field for anti-aging.

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“…First, 50 g of fruit was placed in a glass beaker, and 500 mL of water was poured. The mixture was stirred with a magnetic stirrer for 24 h at 500 rpm according to the procedure described by Gori et al [48] with modifications. Extraction was carried out at room temperature (about 22-24 • C).…”

Section: Maceration With Stirring (Me)mentioning

confidence: 99%

Evaluation of the Antioxidant, Cytoprotective and Antityrosinase Effects of Schisandra chinensis Extracts and Their Applicability in Skin Care Product

et al. 2022

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Plant extracts have been widely used for skin care for many centuries, and nowadays, they are commonly applied for the development and enrichment of new cosmetic preparations. The present study aimed the assessment of the biological activity of aqueous Schisandra chinensis extracts as a potential ingredient of skin care products. The aspects studied involved the ability to neutralize free radicals, impact on viability and metabolism of keratinocytes, as well as tyrosinase inhibitory potential. Our study showed that aqueous S. chinensis extracts have a positive effect on keratinocyte growth and have high antioxidant potential and strong tyrosinase inhibitory activity. UPLC-MS analysis revealed that three groups of phenolic compounds were predominant in the analyzed extract, including lignans, phenolic acids and flavonoids and protocatechiuc and p-coumaryl quinic acids were predominant. Moreover, microwave-assisted extraction, followed by heat reflux extraction, was the most effective for extracting polyphenols. Furthermore, a prototypical natural body washes gel formulation containing the previously prepared extracts was developed. The irritation potential and viscosity were assessed for each of the formulations. The study demonstrated that the addition of these extracts to body wash gel formulations has a positive effect on their quality and may contribute to a decrease in skin irritation. In summary, S. chinensis aqueous extracts can be seen as an innovative ingredient useful in the cosmetic and pharmaceutical industry.

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Development of an innovative maceration technique to optimize extraction and phase partition of natural products

Cited by 19 publications

References 11 publications

Antioxidant Activity of Citrus Limonoids and Investigation of Their Virucidal Potential against SARS-CoV-2 in Cellular Models

Antioxidant Activity of Citrus Limonoids and Investigation of Their Virucidal Potential against SARS-CoV-2 in Cellular Models

Investigation of Chemical Composition and Biological Activities of Ajuga pyramidalis—Isolation of Iridoids and Phenylethanoid Glycosides

Evaluation of the Antioxidant, Cytoprotective and Antityrosinase Effects of Schisandra chinensis Extracts and Their Applicability in Skin Care Product

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