Development of an indirect competitive ELISA for the determination of papaverine

Jin, Yan; Mi, Jian-Qiu; He, Jiantao; Guo, Zhiqiang; Zhao, Meiping; Chang, Wen‐Bao

doi:10.1016/j.talanta.2005.01.001

Cited by 27 publications

(19 citation statements)

References 9 publications

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“…The specificity of the anti-PAP pAb has been demonstrated in our previously reported indirect competitive enzyme-linked immunosorbent assay (ELISA) [14]. The obtained anti-PAP pAb showed high affinity for papaverine with an affinity constant (K aff ) of 7.3610 7 L/mol.…”

Section: Characterization Of the Anti-pap Pabmentioning

confidence: 80%

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An efficient immunoaffinity chromatographic method for extraction and purification of papaverine from samples of pericarpium papaveris and food products

Jin

Zhao²,

2005

J. Sep. Science

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A highly selective immunoaffinity column was obtained by coupling anti-papaverine polyclonal antibodies to CNBr-activated Sepharose 4B. It was found that the coupling efficiency and performance of the immunoaffinity column were greatly improved by prolonging the coupling reaction time from 3 h at 20 degrees C with shaking to incubation overnight at 4 degrees C after the 3 h shaking reaction. The pH and ionic strength were observed to be the most important factors that influence the binding of papaverine to the immunoaffinity column. Using 0.01 mol/L phosphate buffered saline (PBS, pH 8.3) and methanol-water (80:20, v/v) as the loading and eluting solutions, respectively, papaverine was first retained on the column and then quantitatively eluted out with a mean recovery of 86% at a loading concentration of 1 microg/mL. When applied to real samples of pericarpium papaveris and food products, the established immunoaffinity column showed high efficiency in removing the matrix interferences in the samples and satisfactory recovery results were obtained. The method was useful for extraction and purification of papaverine from related samples.

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Section: Characterization Of the Anti-pap Pabmentioning

confidence: 80%

“…The polyclonal antibodies against papaverine were produced and purified according to a previously reported method [14]. In brief, papaverine was first mono-demethylated on the benzyl ring [15], and then activated using bromoacetic acid to obtain a carboxy group.…”

Section: Production and Purification Of Anti-papaverine Polyclonal Anmentioning

confidence: 99%

An efficient immunoaffinity chromatographic method for extraction and purification of papaverine from samples of pericarpium papaveris and food products

Jin

Zhao²,

2005

J. Sep. Science

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“…[13][14][15][16][17][18][19][20][21][22][23] Indirect competitive ELISA was done for the monitoring of papvarine in serum and injection samples with the limit of detection upto 0.25 and 0.06 ng mL À1 . 24 6-Monoacetyl morphine (MAM) screening was done in urine samples by cloned enzyme donor immunoassay upto 410-2010 mg mL À1 .…”

Section: 3mentioning

confidence: 99%

An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples

et al. 2018

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Heroin use and addiction pose serious risks and side effects due to overdose. Quantification of heroin in biological samples is challenging due to rapid deacetylation of heroin to its active metabolites. In this study, we report the quantification of metabolic degradation of heroin by-products in biological urine samples. The presence of the drug was monitored after oral administration of heroin at different time intervals. Various biophysical techniques, such as high performance liquid chromatography (HPLC) and mass spectrometry (MS) were used to evaluate the presence of the drug. A competitive fluorescence based immunoassay was developed with a limit of detection (LOD) up to 0.01 ng mL À1 and the IC 50 value was 0.1 ng mL À1 , while the dipstick assay shows a LOD up to 5 ng mL À1. Rapid detection of narcotic drugs was carried out for biological urine samples collected at various time points. Validation of the developed dipstick was carried out for the standard as well as the spiked urine samples by fluorescence based immunoassay (FIA), using anti-morphine antibodies. A strong correlation (R ¼ 0 .94) was obtained between the developed dipstick and FIA assay for biological urine samples collected at various time points. The developed immunochromatographic dipstick is highly sensitive, field applicable and cost effective, and can serve as a first choice for the monitoring of narcotic drugs in blood, urine and saliva in drug addicts and athletes.

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“…[21][22][23][24] In this work, heterologous icELISA was established using different haptens for coating antigen and immunogen. An intensive investigation for a more The titer was defined as the serum dilution that gave an absobance of 1.0 in noncompetitive condition.…”

Section: Sensitivity and Specificity Of Anti-des Pabsmentioning

confidence: 99%

“…This would lead to significant weakening of the binding ability of antigen-antibody. 24 For the mentioned reason, specificity of the same antibody is always different using icELISA and dcELISA. 20,26 In our established icELISA, coating antigen DES-CM-OVA was immobilized onto a microplate, and limited quantities of free antibodies in liquid phase exposed completely its Fab section to coating antigen and DES leading to an improvement in sensitivity compared to dcELISA to DES establisted by .…”

Section: Sensitivity and Specificity Of Anti-des Pabsmentioning

confidence: 99%

Development of an Indirect Competitive ELISA Based on Polyclonal Antibody for the Detection of Diethylstilbestrol in Water Samples

Wang

Ling

et al. 2007

Chin. J. Chem.

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An indirect competitive enzyme-linked immunosorbent assay (icELISA) based on polyclonal antibody for the estrogen diethylstilbestrol (DES) was developed. With this aim, two different haptens mono-O-3-carboxypropyldiethylstilbestrol (DES-CP) and mono-O-carboxymethyldiethylstilbestrol (DES-CM) with carboxylic group that preserve the molecular structure character of diethylstilbestrol were synthesized. The haptens were conjugated with the carrier proteins bovine serum albumin (BSA) by mixed-anhydride method for immunogen and conjugated with ovalbumin (OVA) by active ester method for coating antigen. Polyclonal antibodies for diethylstilbestrol were raised by immunizing mice with immune antigen DES-CP-BSA. Under optimized system, the lowest limit of detection (LLD) of diethylstilbestrol was 0.01 ng/mL, and IC 50 ＝1.02 ng/mL. Its analogs were tested and no obvious cross-reactivity was found to anti-diethylstilbestrol antibody. DES-fortified water samples were determined by simple dilution to diminish the matrix effect. The comparison between the amount of DES estimated by ELISA and the amount added indicates good agreement for all water samples tested, with mean recovery values ranging from 86% to 120.2%.

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Development of an indirect competitive ELISA for the determination of papaverine

Cited by 27 publications

References 9 publications

An efficient immunoaffinity chromatographic method for extraction and purification of papaverine from samples of pericarpium papaveris and food products

An efficient immunoaffinity chromatographic method for extraction and purification of papaverine from samples of pericarpium papaveris and food products

An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples

Development of an Indirect Competitive ELISA Based on Polyclonal Antibody for the Detection of Diethylstilbestrol in Water Samples

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