Development of an in vitro tissue culture system for hammer coral (Fimbriaphyllia ancora) ovaries

Chiu, Yi‐Ling; Chang, Ching‐Fong; Shikina, Shinya

doi:10.1038/s41598-021-03810-x

Cited by 3 publications

(3 citation statements)

References 60 publications

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“…In the present study, 18 of 26 types of coral cell (e.g., granular gland cells, pigment cells, supporting cells, bipolar neurons, calicoblasts, desmocytes, epitheliomuscular cells, neurons, interstitial cells, interstitial stem cells, absorptive cells, and nutritive–muscular cells) were not identified. These coral cell types can be identified through single-cell RNA sequencing, scanning electron microscopy, transmission electron microscopy, histology, and the use of cell markers [ 37 , 45 , 46 , 48 , 84 , 92 , 93 , 94 , 95 ]. Rosental et al (2017) [ 95 ] and Synder et al (2020) [ 96 ] have used fluorescence-activated cell sorting to separate symbiotic and asymbiotic populations in in vitro cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Coral cell culture began in 1994, when Frank et al obtained cell cultures for multiple coral species; they demonstrated that primary cells could be differentiated [ 35 ]. Coral cell culture was then expanded to other coral species (e.g., [ 24 , 29 , 30 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 ]. Culture media (e.g., Dulbecco’s Modified Eagle’s Medium) and antibiotic-antimycotics were applied to different coral species in well plates or petri dishes with a 12/12-h light cycle and temperature controlled at 23 °C to 26 °C.…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation and Cryobanking of Cells from 100 Coral Species

Toh

Liu

Tsai

et al. 2022

Cells

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When coral species become extinct, their genetic resources cannot be recovered. Coral cryobanks can be employed to preserve coral samples and thereby maintain the availability of the samples and increase their potential to be restocked. In this study, we developed a procedure to determine coral species-specific requirements for cryobank freezing through determining suitable cryoprotective agents (CPAs), CPA concentrations, equilibration times, holding durations, viability rates, and cell amounts for banked coral cells, and we established the first ever coral cell cryobank. Coral cells, including supporting and gland cells, epidermal nematocysts, Symbiodiniaceae and symbiotic endoderm cells (SEC) were found from the extracted protocol. Approximately half of the corals from the experimental corals consisted of spindle and cluster cells. Gastrodermal nematocysts were the least common. The overall concentration of Symbiodiniaceae in the coral cells was 8.6%. Freezing using DMSO as a CPA was suitable for approximately half of the corals, and for the other half of species, successful cell cryopreservation was achieved using MeOH and EG. EG and DMSO had similar suitabilities for Acanthastrea, Euphyllia, Favites, Lobophyllia, Pavona, Seriatopora, and Turbinaria, as did EG and MeOH for Acropora, Echinopyllia, and Sinularia and MeOH and DMSO for Platygyra after freezing. At least 14 straws from each species of coral were cryobanked in this study, totaling more than 1884 straws (0.5 mL) with an average concentration of 6.4 × 106 per mL. The results of this study may serve as a framework for cryobanks worldwide and contribute to the long-term conservation of coral reefs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryopreservation and Cryobanking of Cells from 100 Coral Species

Toh

Liu

Tsai

et al. 2022

Cells

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“…damicornis tissue protein content [105], A. muricata branchiness [98] and Fimbria-phyllia paradivisa gene expression [104]. One study however found that antibiotics decreased mRNA expression of the yolk protein vitellogenin in Fimbriaphyllia ancora cultured ovaries but the study has a high risk of bias due to the presence of a confounding factor [107].…”

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confidence: 99%

What are the toxicity thresholds of chemical pollutants for tropical reef-building corals? A systematic review

et al. 2023

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Background Tropical coral reefs cover only ca. 0.1% of the Earth’s surface but harbour exceptional marine biodiversity and provide vital ecosystem services to millions of people living nearby. They are currently threatened by global (e.g. climate change) and local (e.g. chemical pollution) stressors that interact in multiple ways. While global stressors cannot be mitigated by local actions alone, local stressors can be reduced through ecosystem management. Here, we aimed to systematically review experimental studies assessing the toxicity of chemical pollutants to tropical reef-building corals to generate accessible and usable knowledge and data that can be used to calculate measurement endpoints in ecological risk assessment. From the quantitative estimates of effects, we determined toxicity thresholds as the highest exposures tested at which no statistically significant adverse effects were observed, and we compared them to regulatory predicted no effect concentrations for the protection of marine organisms, to assess whether these reference values are indeed protective of corals. Methods The evidence was taken from a systematic map of the impacts of chemicals arising from human activity on tropical reef-building corals published in 2021. All studies in the map database corresponding to the knowledge cluster “Evidence on the ecotoxicological effects of chemicals on corals” were selected. To identify subsequently published literature, the search was updated using a subset of the search string used for the systematic map. Titles, abstracts and full-texts were screened according to the criteria defining the selected cluster of the map. Because the eligibility criteria for the systematic review are narrower than the criteria used to define the cluster in the systematic map, additional screening was performed. Studies included were critically appraised and each study was rated as low, unclear, medium, or high risk of bias. Data were extracted from the studies and synthesised according to a strategy dependent on the type of exposure and outcome. Review findings The systematic review reports the known effects of chemical exposures on corals from 847 studies corresponding to 181 articles. A total of 697 studies (161 articles) were included in the quantitative synthesis and 150 studies (50 articles) in the narrative synthesis of the findings. The quantitative synthesis records the effects of 2706 exposure concentrations-durations of 164 chemicals or mixtures of chemicals, and identifies 105 toxicity thresholds corresponding to 56 chemicals or mixtures of chemicals. When toxicity thresholds were compared to reference values set for the protection of marine organisms by environmental agencies, the reference values appear to be protective of corals for all but three chemicals assessed: the metal copper and the pesticides diuron and irgarol 1051. Conclusions This open-access database of known ecotoxicological effects of chemical exposures on corals can assist managers in the ecological risk assessment of chemicals, by allowing easy determination of various ecotoxicological thresholds. Several limitations of the toxicity tests synthesised here were noted (in particular the lack of measurement of effective concentrations for more than half of the studies). Overall, most of the currently available data on coral toxicity should be replicated independently and extended to corals from less studied geographical regions and functional groups.

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In vitro detection of marine invertebrate stem cells: utilizing molecular and cellular biology techniques and exploring markers

Mohajer,

Khoradmehr,

Riazalhosseini

et al. 2024

Front. Cell Dev. Biol.

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Marine invertebrate stem cells (MISCs) represent a distinct category of pluripotent and totipotent cells with remarkable abilities for self-renewal and differentiation into multiple germ layers, akin to their vertebrate counterparts. These unique cells persist throughout an organism’s adult life and have been observed in various adult marine invertebrate phyla. MISCs play crucial roles in numerous biological processes, including developmental biology phenomena specific to marine invertebrates, such as senescence, delayed senescence, whole-body regeneration, and asexual reproduction. Furthermore, they serve as valuable models for studying stem cell biology. Despite their significance, information about MISCs remains scarce and scattered in the scientific literature. In this review, we have carefully collected and summarized valuable information about MISC detection by perusing the articles that study and detect MISCs in various marine invertebrate organisms. The review begins by defining MISCs and highlighting their unique features compared to vertebrates. It then discusses the common markers for MISC detection and in vitro techniques employed in invertebrate and vertebrates investigation. This comprehensive review provides researchers and scientists with a cohesive and succinct overview of MISC characteristics, detection methods, and associated biological phenomena in marine invertebrate organisms. We aim to offer a valuable resource to researchers and scientists interested in marine invertebrate stem cells, fostering a better understanding of their broader implications in biology. With ongoing advancements in scientific techniques and the continued exploration of marine invertebrate species, we anticipate that further discoveries will expand our knowledge of MISCs and their broader implications in biology.

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Development of an in vitro tissue culture system for hammer coral (Fimbriaphyllia ancora) ovaries

Cited by 3 publications

References 60 publications

Cryopreservation and Cryobanking of Cells from 100 Coral Species

Cryopreservation and Cryobanking of Cells from 100 Coral Species

What are the toxicity thresholds of chemical pollutants for tropical reef-building corals? A systematic review

In vitro detection of marine invertebrate stem cells: utilizing molecular and cellular biology techniques and exploring markers

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