Development of an in vitro psoriatic skin model by tissue engineering

Psoriatic Skin Models: A Need for the Pharmaceutical Industry

“…After another 7 days of culture, the substitutes are raised to the air-liquid interface to favour cell differentiation and stratification. Finally, biopsies are taken after 21 days of culture at the air-liquid interface, and samples are analyzed using histological, immunohistochemical, physico-chemical or permeability techniques (Jean et al, 2009). showed that self-assembled skin substitutes partially maintained psoriasis-like features such as a thick epidermis, hyperproliferation as well as abnormal cell differentiation of epidermal cells (Jean et al, 2009).…”

“…Finally, biopsies are taken after 21 days of culture at the air-liquid interface, and samples are analyzed using histological, immunohistochemical, physico-chemical or permeability techniques (Jean et al, 2009). showed that self-assembled skin substitutes partially maintained psoriasis-like features such as a thick epidermis, hyperproliferation as well as abnormal cell differentiation of epidermal cells (Jean et al, 2009). In 2011, they demonstrated for the first time that pathological substitutes produced by the self-assembly approach can be treated with an anti-psoriatic molecule and react positively to the treatment such as observed in psoriatic skin in vivo.…”

In Vivo and In Vitro Models of Psoriasis

“…(Saiag, et al, 1985) Skin substitutes + + ? - (Barker, et al, 2004;Konstantinova, et al, 1996) Self-assembly Skin substitutes + + -- (Bernard, et al, 2007;Jean et al, 2009) Table 3. In vitro models of psoriasis…”

“…To be effective, a pathological substitute must mimic as closely as possible, morphological and ultrastructural characteristics of the pathology. At the present time, although some in vitro and in vivo models of psoriasis have been reported to replicate some aspects of the disease, research into psoriasis and the subsequent development of therapeutic strategies have been hindered by the absence of more relevant models (Jean, et al, 2009).…”

“…This work strives to develop and characterize a novel in vitro psoriatic skin model produced by tissue engineering, which could be used to investigate the mechanisms of abnormal keratinocyted growth and to study cell-cell interactions. In this section, previously published results will be discussed (Jean, et al, 2009). …”